Conducting Polymer Based Enzyme Electrodes Fabricated by Invertase and Polyphenol Oxidase

Year 2021, Volume: 4 Issue: 2, 113 - 119, 30.11.2021

Elif Doğan Ayşe Elif Böyükbayram

https://doi.org/10.34088/kojose.790262

Abstract

Novel carbon fiber enzyme electrodes were constructed and evaluated. Fabrication of electrodes was performed via electrochemical deposition of a conducting matrice composed of polythiophene and polypyrrole (PTh–PPy) onto carbon fiber substrates. The enzyme was entrapped into the matrix during electropolymerization. Resultant biosensors represented higher kinetic parameters, Vmax and Km, in comparison to PPy matrice, which are 2.471 ± 0.150 mol min1- electrode1- and 30.60 ± 5.30 mM for invertase, 0.056 ± 0.012 mol min1- electrode1- and 842.00 ± 37.50 mM for polyphenol oxidase respectively. Optimum pH and temperature of the immobilized enzyme within PTh–PPy composite indicates that this matrice provides a more protective environment. The detection limit (LOD) of polyphenols was obtained as 0.037 mg mL1-. Polyphenol oxidase enzyme electrodes were proved to be used for the determination of polyphenolic substances in real samples and the results were confirmed by the Folin-Ciocalteau method

Keywords

Carbon Fiber Electrode, Conducting Polymer, Enzyme Immobilization, Polyphenol Oxidase

Supporting Institution

Karabük University

Project Number

KBÜ-BAP-13/2-YL-034

Thanks

The authors gratefully thank Prof. Dr. Sadi Sen for his valuable contribution to this study and acknowledge Karabuk University Scientific Research Funds for the financial support with project number of KBUBAP-13/2-YL-034.

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