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Sipermetrinin Begonia semperflorens’de Polen Tüpü Oluşumu Üzerindeki Etkisi

Year 2020, Volume: 3 Issue: 2, 160 - 163, 31.12.2020

Abstract

Parazit öldürücü insektisit olan sipermetrin, düşük sulu çözünürlük ve ucucu özelliğe sahiptir. Evde ve açık havada kullanılan böcek öldürücü kimyasalların içerisinde bulunan sentetik bir kimyasaldır. Bu çalışmanın amacı, sipermetrinin ev ve bahçe dekorasyonlarında yaygın olarak kullanılan Begonia semperflorens’in polen tüpü gelişimi üzerinde etkisinin tespit edilmesidir. B.semperflorens’den alınan olgun anterler polen çimlendirme besiyerine (%10 sükroz, %0.01 H3BO3, %0.01 CaCI2, %0.02 MgSO4.7H2O, %0.01 KH2PO4) alındıktan sonra lam üzerine 50µl 0.12 M ve 0.24 M konsantrasyonlarında sipermetrin ile 50 µl polen çimlenme besiyeri konuldu. Kontrol grubunda ise distile su uygulanan örnekler 2., 4. ve 6. saatler sonunda polen çimlenmesi, polen tüpü uzunluğu ve polen tüpü anormalliklerini belirlemek için incelendi. 0.24 M sipermetrin uygulamasında, 4 saat sonunda polen çimlenmesinin %65 olduğu gözlenirken, kontrol grubunda bu değer %39.5 olarak saptandı. Polen tüpü uzunluğunun ise kontrol grubuna göre 0.12 M ve 0.24 M sipermetrin uygulamasında arttığı gözlendi. En fazla polen tüpü anormalliğinin 0.24 M sipermetrin uygulamasında 6. saatin sonunda olduğu tespit edilirken, anormallikteki artışın uygulanan sipermetrin konsantrasyonuna paralel olduğu görüldü. Elde edilen verilere göre, yüksek dozda sipermetrinin polen çimlenmesini arttırdığı ancak polen tüpü uzunluklarını inhibe ettiği ve polen tüpü anormalliklerinin artmasına neden olduğu tespit edildi.

References

  • [1] N. E. Programme, “Cypermethrin,” 1989.
  • [2] Farm chemicals handbook. Meister Pub. Co, 2001.
  • [3] Casida J.E., 1980. Pyrethrum flowers and pyrethroid insecticides, Environmental Health and Perspectives, 34, 189–202.
  • [4] Barlow S., Sullivan F., Lines J., 2001. Risk assessment of the use of deltamethrin on bednets for the prevention of malaria, Food Chemistry and Toxicolgy, 39(5), 407–422.
  • [5] He F., 2000. Neurotoxic effects of insecticides--current and future research: a review., Neurotoxicology, 21(5), 829–835,
  • [6] Sandhu H.S., Brar R.S., 2014. Textbook of veterinary toxicology, 1.th ed. Kalyani Publishers, New Delhi, Hindistan
  • [7] Oudou H.C., Alonso R.M, Jiménez R.M., 2001. Voltammetric Study of the Synthetic Pyrethroid Insecticides Cypermethrin and Deltamethrin and Their Determination in Environmental Samples, Electroanalysis, 13(1), 72–77
  • [8] Begum G., 2005. In vivo biochemical changes in liver and gill of Clarias batrachus during cypermethrin exposure and following cessation of exposure, Pestic. Biochem. Physiol., 82(3), 185–196
  • [9] Suvetha L., Ramesh M., Saravanan M., 2010. Influence of cypermethrin toxicity on ionic regulation and gill Na+/K+-ATPase activity of a freshwater teleost fish Cyprinus carpio, Environ. Toxicol. Pharmacol., 29(1), 44–49
  • [10] Adhikari S., Sarkar B., Chatterjee A., Mahapatra C.T., Ayyappan S., 2004. Effects of cypermethrin and carbofuran on certain hematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton), Ecotoxicol. Environ. Saf., 58(2), 220–226
  • [11] Kessler J.R., Armitage A.M., 1993. Effects of carbon dioxide, light and temperature on seedling growth of Begonia x semperflorens-cultorum, J. Hortic. Sci., 68(682), 281–287
  • [12] Bi H., Guo M., Wang J., Qu Y., Du W., Zhang K., 2018. Transcriptome analysis reveals anthocyanin acts as a protectant in Begonia semperflorens under low temperature, Acta Physiol. Plant., 40(1), 1–12
  • [13] Zhang K.M., Yu H.J., Shi K., Zhou Y.H.,Yu J.Q., Xia X.J., 2010. Photoprotective roles of anthocyanins in Begonia semperflorens, Plant Sci., 179(3), 202–208
  • [14] Jin H.J., Zhou Y.G., Liu H.C., Chen S.F., 2011. Paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Begonia semperflorens, Int. J. Syst. Evol. Microbiol., 61(6), 1350–1355
  • [15] Aksoy Ö., Deveci A., Gonca A., 2013. The Effects of Some Pesticides on Pollen Germination of Begonia semperflorens, J. Funct. Environ. Bot., 3(2), 114–119
  • [16] Wuu K.D., Grant W.F., 1967. Chromosomal Aberrations Induced by Pesticides in Meiotic Cells of Barley, Cytologia, 32(1), 31-41
  • [17] Gillespie S., Long R., Seitz N., Williams N., 2014. Insecticide Use in Hybrid Onion Seed Production Affects Pre- and Postpollination Processes Insecticide Use in Hybrid Onion Seed Production Affects Pre- and Postpollination Processes, 107(1), 29–37.
  • [18] Zambon C.R., Techio V.H., Fernando L., Oliveira D., De Oliveira A.F., Pio R., 2018. Abnormalities induced by agricultural pesticides in the microsporogenesis of olive tree (Olea europaea L.) cultivars, Plant Biosyst., 153(1), 1–9
  • [19] Searcy K.B., Mulcahy D.L., 1985. The parallel expression of metal tolerance in pollen and sporophytes of Silene dioica (L.) Clairv., S. alba (mill.) krause and Mimulus guttatus DC, Theor. Appl. Genet., 69(5–6), 597–602
  • [20] Sawidis T., Reiss H.D., 1995. Effects of heavy metals on pollen tube growth and ultrastructure, Protoplasma, 185(3–4), 113–122
  • [21] Sawidis T., 2008. Effect of cadmium on pollen germination and tube growth in Lilium longiflorum and Nicotiana tabacum, Protoplasma, 233(1–2), 95–106
  • [22] Stebbing A.R.D., 1998. A theory for growth hormesis, Mutat. Res. Mol. Mech. Mutagen., 403(1–2), 249–258
  • [23] Turner A.P., Dickinson N.M., Lepp N.W., Water, air, and soil pollution., 57–58, 1.th ed. Kluwer Academic Publishers
  • [24] Shukla Y., Yadav A., Arora A., 2002. Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin, 182, 33–41, 2002.
  • [25] Benova D.K., Rupova I.M., Iagova A.K., Bineva M.V., 1989. Mutagenic effect of pesticides fastac 10 EK and durs ban 4E studied in a micronucleus test in mouse bone marrow cells, Genetika, 25(12), 2266–2268,
  • [26] Rudek Z., Rozek M., 1992. Induction of micronuclei in tadpoles of Rana temporaria and Xenopus laevis by the pyrethroid Fastac 10 EC., Mutat. Res., 298(1), 25–29
  • [27] Lymphocytes B., 2009. The In Vitro Genotoxic Effects of a Commercial Formulation of a -Cypermethrin in Human Peripheral, 36
  • [28] A. A. Alkahtane et al., 2018. Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells : A Dose- and Time-Dependent Study, 1–9
  • [29] Nema S., Bhargava Y., 2018. Quantitative assessment of cypermethrin induced behavioural and biochemical anomalies in adult zebrafish, Neurotoxicol. Teratol., 68, 57–65
  • [30] Korkmaz V., Güngördü A., Ozmen M., 2018. Comparative evaluation of toxicological effects and recovery patterns in zebrafish (Danio rerio) after exposure to phosalone-based and cypermethrin-based pesticides, Ecotoxicol. Environ. Saf., 160, 265–272, 2018.
Year 2020, Volume: 3 Issue: 2, 160 - 163, 31.12.2020

Abstract

References

  • [1] N. E. Programme, “Cypermethrin,” 1989.
  • [2] Farm chemicals handbook. Meister Pub. Co, 2001.
  • [3] Casida J.E., 1980. Pyrethrum flowers and pyrethroid insecticides, Environmental Health and Perspectives, 34, 189–202.
  • [4] Barlow S., Sullivan F., Lines J., 2001. Risk assessment of the use of deltamethrin on bednets for the prevention of malaria, Food Chemistry and Toxicolgy, 39(5), 407–422.
  • [5] He F., 2000. Neurotoxic effects of insecticides--current and future research: a review., Neurotoxicology, 21(5), 829–835,
  • [6] Sandhu H.S., Brar R.S., 2014. Textbook of veterinary toxicology, 1.th ed. Kalyani Publishers, New Delhi, Hindistan
  • [7] Oudou H.C., Alonso R.M, Jiménez R.M., 2001. Voltammetric Study of the Synthetic Pyrethroid Insecticides Cypermethrin and Deltamethrin and Their Determination in Environmental Samples, Electroanalysis, 13(1), 72–77
  • [8] Begum G., 2005. In vivo biochemical changes in liver and gill of Clarias batrachus during cypermethrin exposure and following cessation of exposure, Pestic. Biochem. Physiol., 82(3), 185–196
  • [9] Suvetha L., Ramesh M., Saravanan M., 2010. Influence of cypermethrin toxicity on ionic regulation and gill Na+/K+-ATPase activity of a freshwater teleost fish Cyprinus carpio, Environ. Toxicol. Pharmacol., 29(1), 44–49
  • [10] Adhikari S., Sarkar B., Chatterjee A., Mahapatra C.T., Ayyappan S., 2004. Effects of cypermethrin and carbofuran on certain hematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton), Ecotoxicol. Environ. Saf., 58(2), 220–226
  • [11] Kessler J.R., Armitage A.M., 1993. Effects of carbon dioxide, light and temperature on seedling growth of Begonia x semperflorens-cultorum, J. Hortic. Sci., 68(682), 281–287
  • [12] Bi H., Guo M., Wang J., Qu Y., Du W., Zhang K., 2018. Transcriptome analysis reveals anthocyanin acts as a protectant in Begonia semperflorens under low temperature, Acta Physiol. Plant., 40(1), 1–12
  • [13] Zhang K.M., Yu H.J., Shi K., Zhou Y.H.,Yu J.Q., Xia X.J., 2010. Photoprotective roles of anthocyanins in Begonia semperflorens, Plant Sci., 179(3), 202–208
  • [14] Jin H.J., Zhou Y.G., Liu H.C., Chen S.F., 2011. Paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Begonia semperflorens, Int. J. Syst. Evol. Microbiol., 61(6), 1350–1355
  • [15] Aksoy Ö., Deveci A., Gonca A., 2013. The Effects of Some Pesticides on Pollen Germination of Begonia semperflorens, J. Funct. Environ. Bot., 3(2), 114–119
  • [16] Wuu K.D., Grant W.F., 1967. Chromosomal Aberrations Induced by Pesticides in Meiotic Cells of Barley, Cytologia, 32(1), 31-41
  • [17] Gillespie S., Long R., Seitz N., Williams N., 2014. Insecticide Use in Hybrid Onion Seed Production Affects Pre- and Postpollination Processes Insecticide Use in Hybrid Onion Seed Production Affects Pre- and Postpollination Processes, 107(1), 29–37.
  • [18] Zambon C.R., Techio V.H., Fernando L., Oliveira D., De Oliveira A.F., Pio R., 2018. Abnormalities induced by agricultural pesticides in the microsporogenesis of olive tree (Olea europaea L.) cultivars, Plant Biosyst., 153(1), 1–9
  • [19] Searcy K.B., Mulcahy D.L., 1985. The parallel expression of metal tolerance in pollen and sporophytes of Silene dioica (L.) Clairv., S. alba (mill.) krause and Mimulus guttatus DC, Theor. Appl. Genet., 69(5–6), 597–602
  • [20] Sawidis T., Reiss H.D., 1995. Effects of heavy metals on pollen tube growth and ultrastructure, Protoplasma, 185(3–4), 113–122
  • [21] Sawidis T., 2008. Effect of cadmium on pollen germination and tube growth in Lilium longiflorum and Nicotiana tabacum, Protoplasma, 233(1–2), 95–106
  • [22] Stebbing A.R.D., 1998. A theory for growth hormesis, Mutat. Res. Mol. Mech. Mutagen., 403(1–2), 249–258
  • [23] Turner A.P., Dickinson N.M., Lepp N.W., Water, air, and soil pollution., 57–58, 1.th ed. Kluwer Academic Publishers
  • [24] Shukla Y., Yadav A., Arora A., 2002. Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin, 182, 33–41, 2002.
  • [25] Benova D.K., Rupova I.M., Iagova A.K., Bineva M.V., 1989. Mutagenic effect of pesticides fastac 10 EK and durs ban 4E studied in a micronucleus test in mouse bone marrow cells, Genetika, 25(12), 2266–2268,
  • [26] Rudek Z., Rozek M., 1992. Induction of micronuclei in tadpoles of Rana temporaria and Xenopus laevis by the pyrethroid Fastac 10 EC., Mutat. Res., 298(1), 25–29
  • [27] Lymphocytes B., 2009. The In Vitro Genotoxic Effects of a Commercial Formulation of a -Cypermethrin in Human Peripheral, 36
  • [28] A. A. Alkahtane et al., 2018. Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells : A Dose- and Time-Dependent Study, 1–9
  • [29] Nema S., Bhargava Y., 2018. Quantitative assessment of cypermethrin induced behavioural and biochemical anomalies in adult zebrafish, Neurotoxicol. Teratol., 68, 57–65
  • [30] Korkmaz V., Güngördü A., Ozmen M., 2018. Comparative evaluation of toxicological effects and recovery patterns in zebrafish (Danio rerio) after exposure to phosalone-based and cypermethrin-based pesticides, Ecotoxicol. Environ. Saf., 160, 265–272, 2018.
There are 30 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Makaleler
Authors

Özlem Aksoy 0000-0003-0969-5171

Salih Dikilitaş This is me 0000-0002-4516-3407

Sibel Sütlüoğlu This is me 0000-0002-0381-1613

Duygu Aydın This is me 0000-0003-4909-3562

Publication Date December 31, 2020
Acceptance Date September 29, 2020
Published in Issue Year 2020 Volume: 3 Issue: 2

Cite

APA Aksoy, Ö., Dikilitaş, S., Sütlüoğlu, S., Aydın, D. (2020). Sipermetrinin Begonia semperflorens’de Polen Tüpü Oluşumu Üzerindeki Etkisi. Kocaeli Üniversitesi Fen Bilimleri Dergisi, 3(2), 160-163.