The functions of eicosanoids on nodulation reactions to two fungal isolates in adults of the German cockroach, Blatella germanica (L.)

Yıl 2017, Cilt: 20 Sayı: 2, 111 - 114, 04.01.2017

Hasan Tunaz , Ali Arda Işıkber , Hacı Ali Pur Mehmet Kubilay Er

Öz

One of the
cellular defense reactions to microbial infections in insects is nodulation reactions. In this study, the role of
eicosanoids on nodulation reactions to two fungal isolates (Beauveria bassiana 1512 and Metarhizium anisopliae S8-2) in adults of the German cockroach, Blatella germanica (L.)
(Dictyoptera: Blattellidae) was tested. Treating adult of German
cockroach with Beauveria 1512 and Metarhizium S8-2 induced nodulation reactions. Injecting adults of B. germanica with eicosanoid biosynthesis inhibitors, immediately
before intrahemocoelic injections of the fungal isolates reduced the
nodulation response to fungal challenges. Separate treatments with specific
inhibitors including dexamethasone (a phospholipase A₂ inhibitor),
indomethacin, naproxen, ibuprofen (cyclooxygenase inhibitors), esculetin (a
lipoxygenase inhibitor) and Phenidone (dual cyclooxgenase/lipoxygenase
inhibitor) also impaired the ability of B. germanica to form nodules in reaction to fungal
challenge. These findings support the hypothesis that eicosanoids has a role on
nodulation reactions to the fungal infections in adults of B. germanica.

Anahtar Kelimeler

Insect immunology; Nodulation; Beauveria bassiana; Metarhizium anisopliae; Blatella germanica

Alman hamamböceği Blatella germanica (L.)'nın erginlerinde iki fungal izolata karşı eikosanoidlerin nodülasyon reaksiyonları üzerine olan fonksiyonu

Öz

Böceklerde mikrobiyal enfeksiyonlara karşı
hücresel savunma reaksiyonlarından biri nodülasyon reaksiyonlarıdır. Bu
çalışmada, Alman hamamböceği, Blatella germanica
(L.) (Dictyoptera: Blattellidae)'nın erginlerinde eikosanoidlerin, iki fungal
izolata (Beauveria bassiana'nın 1512 ve Metarhizium anisopliae'nin S8-2) nodülasyon reaksiyonları üzerindeki rolü test edilmiştir.
Ergin böcekler 1512 ve S8-2 ile enfekte edildiğinde, böcekler bu enfeksiyona
karşı nodülasyon reaksiyonu göstermişlerdir. Ergin hamamböceklerine fungal izolatlarla enfekte edilmeden hemen önce eikosanoid
biyosentez inhibitörleri enjekte edilmesi, fungal izolatlara karşı nodülasyon
tepkisini azaltmıştır. Eikosanoid biyosentezindeki enzimlerin  inhibitörlerinden deksametazon (bir
fosfolipaz A2 inhibitörü), indometasin, naproksen, ibuprofen (siklooksijenaz
inhibitörleri), esculetin (bir lipoksijenaz inhibitörü) ve fenidon (hem
siklooksijenaz / hemde lipoksigenaz inhibitörü) de dahil olmak üzere spesifik
inhibitörlerle yapılan ayrı uygulamalar, B.
germenica'nın fungal enfeksiyona karşı nodül oluşturmasını azaltmıştır. Bu
bulgular, eikosanoidlerin B. germenica'nın
erginlerindeki fungal enfeksiyonlara karşı nodülasyon reaksiyonlarında rol
aldığı hipotezini desteklemektedir.

Anahtar Kelimeler

Böcek hücresel bağışıklığı; Nodülasyon; Beauveria bassiana; Metarhizium anisopliae; Blatella germanica

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