Determination of the effect of essential oil of rosemary plant against Alternaria brassicicola in in vitro conditions

Abstract

Alternaria brassicicola is one of the most important pathogens that cause disease in plants belonging to the cabbage family. Cultural measures, chemical and biological control methods are used against this pathogen. Many studies have been conducted on the effects of plant essential oils against fungal pathogens that cause disease in plants. This study was carried out to determine the antifungal effects of rosemary essential oil against A. brassicicola in in vitro conditions. The essential oil was obtained by using the hydrodistillation method in the Clevenger apparatus. The antifungal effects of essential oil against A. brassicicola were tested at concentrations of 10 µl/20ml PDA, 20 µl/20ml PDA, 40 µl/20ml PDA, 80 µl/20ml PDA, 160 µl/20ml PDA and 320 µl/20ml PDA. According to the results, rosemary essential oil showed a strong antifungal effect at doses of 80 µl/20ml PDA and above. However, no effect was obtained at all doses in the detached leaf trials.

Keywords

• antifungal
• plant pathogen
• medicinal and aromatic plant

Biberiye bitkisine ait uçucu yağın in vitro koşullarda Alternaria brassicicola etmenine karşı etkilerinin belirlenmesi

Abstract

Alternaria brassicicola lahanagiller familyasına ait bitkilerde hastalığa neden olan en önemli patojenlerden birisidir. Bu etmene karşı kültürel önlemler, kimyasal ve biyolojik mücadele yöntemleri kullanılmaktadır. Bitki uçucu yağlarının bitkilerde hastalık oluşturan fungal etmenlere karşı etkileri üzerine çeşitli araştırmalar yapılmaktadır. Bu çalışma biberiye bitkisine ait uçucu yağın A. brassicicola etmenine karşı antifungal etkilerini belirlemek amacıyla in vitro koşullarda yürütülmüştür. Clevenger cihazında hidrodistilasyon yöntemi kullanılarak uçucu yağ elde edilmiştir. Uçucu yağın A. brassicicola etmenine karşı antifungal etkileri 10 µl/20ml PDA, 20 µl/20ml PDA, 40 µl/20ml PDA, 80 µl/20ml PDA, 160 µl/20ml PDA ve 320 µl/20ml PDA kosantrasyonlarda denenmiştir. Elde edilen sonuçlara göre biberiye uçucu yağı 80 µl/20ml PDA ve üzerindeki dozlarda güçlü antifungal etki göstermiştir. Ancak koparılmış yaprak denemelerinde tüm dozlarda etki elde edilememiştir.

Keywords

• antifungal
• bitki patojeni
• medicinal and aromatic plant

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