Bisfenol A’ya maruz kalan erkek Yeni Zelanda tavşanlarında punikalajinin potansiyel etkileri

Year 2022, , 259 - 268, 30.09.2022

Özlem Yildiz Gülay , Gülşah Candan , Mehmet Şükrü Gülay

https://doi.org/10.30607/kvj.1071761

Abstract

Çalışmada, BPA verilen Yeni Zelanda tavşanlarında punikalajininbazı oksidan-antioksidan enzimler ile bazı
biyokimyasal parametreler üzerine olası etkilerin incelendi. Bu amaçla 2 hafta boyunca laboratuvar koşullarına
alıştırılan tavşanlar, her grupta 6 tavşan olacak şekilde rastgele 4 gruba ayrıldı: Kontrol (C; mısır yağı ve distile su),
BPA (BPA; mısır yağı içerisinde 20 mg/kg BPA ve distile su), punikalajin (PUN; mısır yağı ve distile su içerisinde
2 mg/kg punikalajin), ve BPA-punikalajin grubu (B+P; mısır yağı içerisinde 20 mg/kg BPA ve distile su içerisinde
2 mg/kg PUN). Uygulamalar 9 hafta boyunca günlük olarak yapıldı ve haftada bir kez yapılan tartımlara göre
dozlar ayarlandı. Çalışma sonunda alınan kan ve doku örneklerinden hematolojik, biyokimyasal ve oksidanantioksidan parametrelerin ölçümleriyapıldı. Analizler neticesinde plazma bilirubin, albümin ve toplam plazma
protein düzeyleri ile Mg, P, Ca, Na, K, seviyelerinde herhangi bir istatistiki farka rastlanmazken, farklı gruplardaki
plazma, karaciğer ve böbrek glutatyon peroksidaz değerleri de önemsiz bulundu (p>0,1). Oral BPA uygulamaları
serum kolesterol, LDL, HDL, amilaz, lipaz, CRP, GGT seviyeleri ile karaciğer ve böbrek dokusundaki
malonildialdehit, katalaz ve süperoksit dismütaz seviyelerini olumsuz etkiledi (p<0,05). B+P grubunda ise bu
parametrelerde önemli ölçüde iyileşme gözlendi. Elde edilen sonuçlar, BPA'nın erkek tavşanlarda yol açtığı olası
toksik etkilerin punikalajin tedavisi ile önemli ölçüde düzeltilebileceğini gösterdi.

Keywords

Antioksidanlar, Kolesterol, Oksidan-Antioksidan enzimler, Polifenoller, Toksikasyon

Supporting Institution

TÜBİTAK

Project Number

116O027

Potential effects of punicalagin on New Zealand White rabbits exposed to bisphenol A

Abstract

The possible effects of punicalagin on some oxidant-antioxidant enzymes and biochemical parameters in
bisphenol A (BPA)-treated rabbits were investigated. Animals were randomly divided into 4 groups, each
containing 6 rabbits: Control (C; corn oil and distilled water), BPA (BPA; 20 mg/kg BPA in corn oil and distilled
water), the punicalagin (PUN; corn oil and 2 mg/kg punicalagin in distilled water), and BPA-punicalagin (B+P; 20
mg/kg BPA in corn oil and 2 mg/kg PUN in distilled water) groups. Daily treatments continued for 9 weeks and
doses were adjusted according to body weights measured for each week. At the end of the study, hematological,
biochemical, and oxidant-antioxidant parameters were measured from blood and tissue samples. The difference
in the levels of plasma bilirubin, albumin, total plasma protein, Mg, P, Ca, Na, K, and levels of glutathione
peroxidase in plasma, liver, and kidney were non-significant (p>0.1). However, oral BPA administration adversely
affected serum cholesterol, LDL, HDL, amylase, lipase, CRP, and GGT concentrations. Likewise,
malondialdehyde, catalase, and superoxide dismutase levels in the kidney and liver were also negatively altered by
BPA (p<0.05). Significant improvements in these parameters were apparent in the B+P group. The data
generated here showed that punicalagin possessed a beneficial impact on potentially reducing the possible toxic
effects of BPA in rabbits. 

Keywords

Antioxidants, Cholesterol, Oxidant-Antioxidant enzymes, Polyphenols, Toxication

Project Number

116O027

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