LPS ile Indüklenen Septisemik Koyunlarda Bazı Eser Element Konsantrasyonlarının İncelenmesi

Year 2022, , 297 - 302, 30.09.2022

Fulya Altınok Yipel , Mustafa Yipel

https://doi.org/10.30607/kvj.1123763

Cited By: 1

Abstract

Enfeksiyona karşı düzensiz immünolojik konak yanıtına bağlı meydana gelen organ bozukluğu olarak tanımlanan endotoksemi, koyunlarda yüksek mortalite ve morbidite sonucu ciddi ekonomik kayıplara neden olan bir hastalıktır. Çalışmada septisemi oluşturulmayan sağlıklı (kontrol) koyunlar (n=6) ile septisemi oluşturulan koyunlardan (n=6) alınan plazma ve serum örneklerinde makro (Ca, Mg, Na, K, P) ve mikro (Fe, Cu, Se, Zn) esansiyel element konsantrasyonları belirlendi. Sonuçlara göre Ca, K ve Se konsantrasyonları açısından gruplar arasındaki farklar istatistiksel olarak anlamlıydı (p≤0.05). Septik hastalarda başarılı teşhis ve tedavi, yeni biyobelirteç belirlenebilmesi ve mekanizmalarının açıklanabilmesi açısından ICP-MS gibi hassas analiz yöntemlerinin kullanıldığı elementler konsantrasyonları ve değişimleri üzerine kapsamlı çalışmalara ihtiyaç duyulmaktadır.

Keywords

ICP-MS, Serum.

Investigation of the Concentrations of Some Essential Elements in LPS-Induced Septicemic Sheep

Abstract

Endotoxemia, which is defined as an organ disorder due to irregular immunological host response to infection, is a disease that causes serious economic losses as a result of high mortality and morbidity in sheep. In the study, macro (Ca, Mg, Na, K, P) and micro (Fe, Cu, Se, Zn) essential element concentrations of plasma and serum samples; taken from healthy (control) (n=6) and septicemic sheep (n=6) were determined. According to the results, the differences between the groups in terms of Ca, K, and Se concentrations were statistically significant (p≤0.05). Comprehensive studies on the concentrations and changes of elements using sensitive analysis methods such as ICP-MS are needed to successful diagnose and treat septic patients, identify new biomarkers, and explain their mechanisms.

Keywords

Septicemia, ICP-MS, Macro element, Micro element, Plasma, Serum.

References

• Ali H, Khan E. What are heavy metals? Long-standing controversy over the scientific use of the term ‘heavy metals’–proposal of a comprehensive definition. Toxıcol Envıron Chem. 2018; 100(1): 6-19.
• Beisel WR. Trace elements in infectious processes. Med. Clin. North Am. M. 1976; 60(4): 831-849.
• Cedeño Y, Miranda M, Orjales I, Herrero-Latorre C, Suárez M, Luna D, & López-Alonso M. Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs. Animals. 2020; 10(12): 2316.
• Erdoğan S, Erdoğan Z, Şahin N. Mevsimsel olarak merada yetiştirilen koyunlarda serum bakır, çinko ve seruloplazmin konsantrasyonları ile yün bakır ve çinko değerlerinin araştırılması. Ankara Üniv Vet Fak Derg. 2003; 5O: 7-11.
• Etzel KR, Swerdel MR, Swerdel JN, Cousins RJ. Endotoxin-induced changes in copper and zinc metabolism in the Syrian hamster. J. Nutr.1982; 112(12): 2363-2373.
• Fan Q, Wang Z, Chang S, Peng Z, Wanapat M, Bowatte S, Hou F. Relationship of mineral elements in sheep grazing in the highland agro-ecosystem. Asian-australas. J. Anim. Sci. 2020; 33(1): 44.
• Goyer R. Issue paper on the human health effects of metals. US Environmental Protection Agency.2004.
• Humann-Ziehank E, Ganter M, Hennig-Pauka I, Binder A. Trace mineral status and liver and blood parameters in sheep without mineral supply compared to local roe deer (Capreolus capreolus) populations. Small Ruminant Res. 2008; 75: 185-191.
• Huo B, Wu T, Song C, Shen X. Studies of selenium deficiency in the Wumeng semi-fine wool sheep. Biol. Trace Elem. Res. 2020; 194(1): 152-158.
• Kaneko JJ, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals. Academic press.2008.
• Konukoglu D, Ercan M, Ziylan E. Trace element levels in the experimental peritonitis. J. Trace Elem. Med. Biol. 2001; 15(2-3): 115-118.
• Mert H, Mert N, Dogan I, Cellat M, & Yasar S. Element status in different breeds of dogs. Biol. Trace Elem. Res. 2008; 125(2): 154-159
• Middleton JR, Luby CD, Viera L, Tyler JW, Casteel S. Influence of Staphylococcus aureus intramammary infection on serum copper, zinc, and iron concentrations. Int. J. Dairy Sci. 2004; 87(4): 976-979.
• Naghadeh DB, Bahrami Y, Rezaei AS, Anassori E, Janalipour A. et al. (2015). Platelet Indices of Selenium Status in Healthy and Selenium-Deficient Sheep: a Comparison with Selenium Indices in Plasma, Whole Blood, and Red Blood Cells. Biol Trace Elem Res. 2015; 168: 74-81.
• Nordberg GF, Fowler BA, Nordberg M. Handbook on the Toxicology of Metals. Academic press. USA. 2015.
• Pamukçu T, Sel T, Yarim G. Blood serum concentrations of selenium and glutathione peroxidase activity in Akkaraman sheep. Turkish J. Vet. Anim. Sci. 2001; 25(5): 731-734.
• Patrashkov SA, Petukhov VL, Korotkevich OS, Petukhov IV. Content of heavy metals in the hair. In: Journal de Physique IV (Proceedings). EDP sciences. 2003; 107: 1025-1027.
• Puls R. Normal levels summary sheets. In: Mineral levels in animal health: diagnostic data, 2nd ed., Sherpa International, Clearbrook, British Columbia, Canada. 1994; pp. 302-353.
• Radostits OM, Gay C, Hinchcliff KW, Constable PD. Veterinary Medicine E-Book: A textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences. 2006.
• Redl H, Bahrami S, Schlag G, & Traber DL. Clinical detection of LPS and animal models of endotoxemia. Immunobiology. 1993; 187(3-5): 330-345.
• Riquelme JB, Cazanga VM, Palma CJ, Castillo LK, & Pérez R. Clinical and hematological changes in sheep induced by Escherichia coli lipopolysaccharide. Arq. Ciênc. Vet. Zool. UNIPAR. 2018; 21(2): 47-53.
• Strachan S. Trace elements. Curr Anaesth Crit Care. 2010; 21(1): 44-48.
• Saner G, Uğur Baysal S, Ünüvar E, Özden T. Serum zinc, copper levels, and copper/zinc ratios in infants with sepsis syndrome. J. Trace Elem. Exp. Med. 2000; 13(3): 265-270.
• Tatara MR, Łuszczewska-Sierakowska I, Krupski W. Serum concentration of macro-, micro-, and trace elements in Silver fox (Vulpes vulpes) and their interrelationships with morphometric, densitometric, and mechanical properties of the mandible. Biol Trace Elem Res. 2018; 185(1): 98-105.
• Tuncer SS, Denizhan V, Kozat S. Investigation of Serum and Wool Levels of Cobalt, Manganese, Selenium and Zinc in Liver-Trematode-Infected Sheep. Kocatepe Vet. J. (2020); 13(2): 185-191.
• Türkmen Ü, A. Sepsis-Değişiklikler. Aydın Sağlık Dergisi, 2017; 3 (2): 9-17.
• Underwood EJ, Suttle NF. The mineral nutrition of livestock. London, UK: CABI Publishing; 1999.
• Vázquez-Armijo JF, Rojo R, López D, Tinoco JL, González A, Pescador N, Domínguez-Vara IA. Trace elements in sheep and goats reproduction: a review. Trop. Subtrop Agroecosystems. 2011; 14(1): 1-13.
• Yeltekin AÇ, Karapinar Z, Mis L. The changes in the levels of elements in sheep with Contagious Ecthyma. Indian J. Anim. Res. 2018; 52(1): 56-60.
• Zoroddu MA, Aaseth J, Crisponi G, Medici S, Peana M, Nurchi VM. The essential metals for humans: a brief overview. J. Inorg. Biochem. 2019; 195: 120-129.