Tek tırnaklı hayvanlarda Kırım Kongo Hemorajik Ateşi Virusu'na spesifik antikorların tespiti

Abstract

Kırım Kongo kanamalı ateşi (KKKA) virus enfeksiyonu, dünyanın geniş bir bölgesinde endemik olan ve özellikle insan sağlığını tehdit eden, potansiyel olarak ölümcül bir hastalık olan kene kaynaklı zoonotik bir hastalıktır. Hastalığın bir bölgede endemik olduğunun ana göstergelerinden biri, hayvan popülasyonlarında KKKAV'ye özgü antikorların varlığıdır. Birçok hayvan türü KKKAV'yi asemptomatik olarak taşıyabilir ve bu nedenle hastalığın bulaşma döngüsüne katılabilir. Serolojik çalışmalar, doğada KKKAV' nin hayatta kalması için tek tırnaklıların önemli olduğunu göstermiştir. Ancak Türkiye’de bu konuda daha fazla çalışmaya ihtiyaç vardır. Araştırma için Afyonkarahisar ve Burdur illerinde çeşitli cinsiyet ve yaştaki 11 at ve 86 eşekten kan örnekleri alındı. Yetiştiriciler tarafından çeşitli amaçlarla tutulan bu hayvanların kan serumlarında KKKV'ye özgü antikorların varlığı araştırıldı. Spesifik antikorların tespiti için hızlı ve güvenilir bir yöntem olan çift antijenli ELISA test yöntemi kullanıldı. Sonuç olarak toplamda %51.54 (50/97) seropozitiflik tespit edildi. Bulgular, tek tırnaklı hayvanların KKKA'nın epidemiyolojisinde rezervuar olarak önemli bir rol oynayabileceğini göstermektedir.

Keywords

• ELISA
• equide
• eşek
• Kırım Kongo Kanamalı Ateşi Virusu
• seroprevalans

Crimean Congo hemorrhagic fever virus-specific antibody detection in Equids

Abstract

Crimean-Congo hemorrhagic fever (CCHF) virus infection is a tick-borne zoonotic disease that is endemic in a large region of the world and is a potentially fatal disease, especially threatening human health. One of the main indicators that the disease is endemic in a region is the presence of antibodies specific to CCHFV in animal populations. Many animal species may carry CCHFV asymptomatically and hence participate to the disease's transmission cycle. Serological studies have shown that equids are important to the survival of CCHFV in nature. Nevertheless, need for more studies on this subject in Türkiye. For this study, blood samples were taken from 11 horses and 86 donkeys of different sex and variable ages in the provinces of Afyonkarahisar and Burdur. The presence of CCHFV-specific antibodies in the blood serum of these animals, which are held by the breeders for a variety of purposes, was studied. The double-antigen ELISA test method, which is a fast and reliable method, was used for the detection of specific antibodies. As a result, a total of 51.54% (50/97) seropositivity was detected. Findings indicate that equidae may play an important role as a reservoir in the epidemiology of CCHFV.

Keywords

• Crimean-Congo hemorrhagic fever
• donkey
• ELISA
• equide
• seroprevalence

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