Identification and Antimicrobial Susceptibility of Microorganisms Isolated from External Ear Canal of Healthy Dogs

Abstract

Keywords

• -

Sağlıklı Köpeklerin Dış Kulak Kanalından İzole Edilen Mikroorganizmalar ve Antibiyotik Duyarlılıkları

Abstract

Bu çalı ma sa lıklı 141 köpe in dı kulak kanalının mikrobiyolojik muayenesi ve antibiyotik duyarlılıklarının belirlenmesi amacıyla planlandı. Köpek kulaklarından en yüksek oranda Staphylococcus aureus (%31.5) identifiye edildi. Bu mikroorganizmayı sırasıyla Streptococcus spp. (%16.4), Bacillus spp.(%12.3), Staphylococcus intermedius (%9.5), Proteus spp. (%8.2), Pseudomonas aeruginosa (%8.2), Escherichia coli (%6.8), Candida spp. (%4.2) ve Aspergillus spp. (%3.5) izledi. statistiksel olarak sa lıklı köpeklerin kulak kanalından izole edilen mikroorganizmalar arasındaki farklılık önemli bulundu (p 0.05). zole edilen su lara antibiyotik duyarlılık trestleri yapıldı. Staphylococcus aureus, Streptococcus spp. ve Staphylococcus intermedius türlerinin büyük ço unlu u amoksisilin/klavulanik asit, ceftiofur ve gentamisine yüksek oranda duyarlı bulundu. Sonuç olarak otitis eksterna tedavisinde gentamisin, amoksisilin/klavulanik asit ve antifungal kombinasyonlarının kullanılmasının yararlı olabilece i kanısına varıldı

Keywords

• -

References

1. Lyskova P, Vydrzalova M, Mazurova J (2007) Identification and antimicrobial susceptibility of bacteria and yeasts isolated from healthy dogs and dogs with otitis externa. J Vet Med A, 54: 559-563.
2. Keskin O, Kökçü L, Akan M (1999) Otitis eksternalı köpeklerden izole edilen mikroorganizmalar ve antibiyotik duyarlılıkları. Ankara Üniv Vet Fak Derg, 46: 163-168.
3. Oliveira LC, Leite CAL, Brilhante RSN, Carvalho CBM (2008) Comparative study of the microbial profile from bilateral canine otitis. Can Vet J, 49: 785-788.
4. Fernandez G, Barboza G, Villalobos A, Para O, Finol G, Ramirez RA (2006) Isolation and identification of microorganisms present in 53 dogs suffering otitis externa. Rev Cient-Fac Cien V, 16:23-30.
5. Blanco JL, Guedeja-Marron J, Blanco I,Garcia ME (2000) Optimum incubation codition for the isolation of yeasts from canine otitis externa. J Vet Med B, 47:599-605.
6. Doyle RS, Skelly C, Bellenger CR (2004) Surgical management of 43 cases of chronic otitis externa in the dog. Ir Vet J, 57: 22-30.
7. Bensignor E, Legeay D, Medaille C (2000) Prospective study on otitis externa in the adult dog in France. Prat Med Chir Anim, 35: 405-414.
8. May ER, Hnilica KA, Frank LA, Jones RD, Bemis DA (2005) Isolation of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma, or both. J Am Vet Med Assoc, 227: 928-931.

9. Martin Barrasa JL, Lupiola Gomez P, Gonzales Lama Z, Tejedor Junco TM (2000) Antibacterial susceptibility pattern of Pseudomonas strains isolated from chronic canine otitis externa. J Vet Med B, 47:191-196.
10. Tejedor Junco TM, Martin Barrasa JL (2002) Identification of antimicrobial susceptibility of coagulase positive Staphylococci isolated from healthy dogs and dogs suffering from otitis externa. J Vet Med B, 49:419-423.
11. Akay Ö, Aslanbey D, Arda M, Canda A, Aydın N, zgür M, Diker S (1984) Otitis externalı köpeklerden izole edilen mikroorganizmalar ve bunların antibiyotiklere duyarlılıkları üzerine bir ara tırma. A Ü Vet Fak Derg, 31: 452-462.
12. Crespo MJ, Abarca ML, Cabanes FC (2000) Atypical lipid dependent Malassezia species isolated from dogs with otitis externa. J Clin Microbiol, 38: 2383-2385. Kumar A, Singh K, Sharma A (2002) Prevalence of Malassezia pachydermatitis and other organisms in healthy and infected dogs ears. Isr Vet Med Assoc, 57: 145-148. Guardabassi L, Schwarz S, Lloyd DH (2004). Pet animals as reservois of antimicrobial resistant bacteria. J Antimicrob Chemother. 54: 321-332.
13. Lilenbaum W, Veras M, Blum E, Souza GN (2000) Antimicrobial susceptibility of staphylococci isolated from otitis externa in dogs. Lett Appl Microbiol, 31(1): 42-45.
14. Quinn P J, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2002). Veterinary Microbiology and Microbial Disease. Blackwell Publishing Professional, Iowa.
15. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Facultatively Anaerobic Gram-negative Rods (in) Bergey’s Manual of Determinative Bacteriology, 9th Ed., 175-290, Williams and Wilkins, 428 East Preston Street, Baltimore, Maryland 21202, USA.
16. National Committee for Clinical Laboratory Standards (2001) Performance Standards for Antimicrobial Susceptibility Testing. Eleventh Informational Supplement, NCCLS document 100-S11, Weyne, PA, 21.
17. Kiss G, Radvanyi S, Szigeti G (1997) New combination for the therapy of canine otitis externa. I. Microbiology of otitis externa. J Small Anim Pract, 38-51-56.
18. Hariharan H, Coles M, Poole D, Lund L, Page R (2006) Update on antimicrobial susceptibilities of bacterial isolates from canine and feline otitis externa. Can Vet J, 47: 253-255.
19. Pena Bi Varges R, Medeiros L, Martins GM, Martins Rr, Lilenbaum W (2010) Species distribution and antimicrobial susceptibility of staphylococci isolated from canine otitis externa. Vet Dermatol, 21(3): 292-296.
20. Becker K, Keller B, Eiff C (2001) Enterotoxigenic potential of Staphylococcus intemedius. Appl Environ Microbiol, 67: 5551-5557.
21. Hendricks A, Schuberth H, Schueler K, Lloyd DH (2002) Frequency of superantigen-producing Staphylococcus intemedius isolated from canine proderma and proliferation-inducing potential of superantigen in dogs. Res Vet Sci, 73: 273-277.
22. Teraucki R, Sato H, Hasegawa T, Yamaguchi T, Aizawa C, Maehara N (2003) Isolation of exfoliative toxin from Staphylococcus intemedius and its local toxicity in dogs. Vet Microbiol, 94: 19-29.
23. Yoshida N, Naito F, Fukata T (2002) Studies of certain factors affecting the microenvironment and microflora of the external ear of the dog in health and disease. J Vet Med Sci, 64: 1145-1147. Graham –Mize CA, Rosser EJ, (2004) Comparison of microbial isolates and susceptibility from external ear canal of dogs with otitis externa. J Am Anim Hosp Assoc, 40: 102-108.