Detection of Listeria Monocytogenes in Raw Milk Samples Retailed in Kayseri by Classical Cultural Technique

Abstract

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Kayseri’de Satışa Sunulan Çiğ Süt Numunelerinde Listeria Monocytogenes Varlığının Klasik Kültür Yöntemi ile Belirlenmesi

Abstract

Bu çalı mada Kayseride açıkta satılan çi süt örneklerinde Listeria monocytogenes varlı ını ortaya koymak amaçlanmı tır. Bu amaçla Mayıs-Eylül 2009 aylarında farklı sokak sütçüleri tarafından satı a sunulan toplam 50 adet çi süt örne i ISO 11290-1/A1-2004da önerilen L. monocytogenes izolasyon prosedürüne göre analiz edilmi tir. Elde edilen izolatlar Microbact test kitinde biyokimyasal özellikleri açısından de erlendirilerek tür tayini yapılmı tır. Örneklerden 11inin (%22) Listeria türleri ile kontamine oldu u, bunlardan 6sının (%12) sadece L monocytogenes ile, 3 tanesinin (%6) sadece L. ivanovii ile, 1 tanesinin(%2) sadece L. seeligeri ile, 1 tanesinin(%2) ise hem L. monocytogenes hem de L. grayi ile kontamine oldu u belirlenmi tir. Genel olarak 50 çi süt örne inden toplam 7 tanesinin (%14) L. monocytogenes ile kontamine oldu u, 39unun (%78) Listeria türlerini içermedi i ortaya konmu tur. Çalı mada elde edilen verilere göre, Kayseride sokakta satılan çi sütlerden kaynaklanan mikrobiyolojik riskin, çi sütün ne amaçla kullanılaca ına göre de erlendirilmesi gerekti i, ısı i lemi uygulanarak süt ürünü yapımında kullanılacaksa riskin minimal düzeylerde kalırken, ısı i lemi uygulanmadan direk tüketim söz konusu oldu unda çi sütlerde L. monocytogenes varlı ının halk sa lı ı açısından bir tehlike yaratabilece i sonucuna varılmı tır. Dolayısıyla sonuçlar Kayseride satı a sunulan çi süt örneklerinin L. monocytogenes açısından göz ardı edilmemesi gereken potansiyel bir halk sa lı ı risk unsuru oldu unu ortaya koymaktadır

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References

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