Sıçanlarda Poliklorlu Bifenil (Aroklor 1254) ile Oluşturulan Nefrotoksisite Üzerine Alfa Lipoik Asitin Etkisi

Year 2023, , 504 - 512, 31.12.2023

Mustafa Yıldız

https://doi.org/10.30607/kvj.1364111

Abstract

Sunulan çalışma, PCB (Aroklor 1254)’ye maruz bırakılan sıçanların böbreklerindeki morfolojik değişikliklerin belirlenmesini ve bu değişiklikler üzerine ALA'nın etkilerinin ortaya çıkarılmasını amaçladı. Sıçanlar kontrol, ALA, PCB ve ALA+PCB olmak üzere rastgele dört gruba ayrıldı. ALA ve PCB uygulamaları otuz gün boyunca devam etti. Deney sonunda hayvanlardan doku örnekleri alındı. Histopatolojik ve histomorfometrik incelemeler için böbrek kesitlerine Crossmon’un üçlü boyama yöntemi uygulandı. Ayrıca doku kesitlerinde periyodik asit-Schiff (PAS) reaksiyonu incelendi. Histopatolojik açıdan, böbrekte glomerüler dejenerasyon ile bağ doku yoğunluklarının kontrol grubuna göre PCB grubunda anlamlı bir şekilde arttığı tespit edildi. Bununla birlikte, glomerüler dejenerasyon yoğunluğunun PCB grubuna göre ALA+PCB grubunda anlamlı bir şekilde azaldığı saptandı. Böbrekte renal korpüskül, proksimal tübül, çıkan henle ve toplayıcı kanal çaplarının kontrol grubuna göre PCB grubunda anlamlı bir şekilde azaldığı belirlendi. Ancak bu parametreler PCB grubuna göre ALA+PCB grubunda anlamlı bir şekilde arttı. Böbrekte glomerüler mezengial matriks yoğunluğu ile proksimal tübül epitelindeki glikojen yoğunluğu kontrol grubuna göre PCB grubunda anlamlı bir şekilde artarken, bu parametreler PCB grubuna göre ALA+PCB grubunda anlamlı bir şekilde azaldı. Sonuç olarak, PCB böbrek histolojisinde bozukluklara neden olarak renal fonksiyonları olumsuz etkileyebilir. Bununla birlikte, PCB maruziyetine karşı ALA kullanımı böbrek dokusunun korunmasına katkı sağlayabilir.

Keywords

Alfa lipoik asit, Aroklor 1254, Böbrek, Histoloji, Sıçan

Effect of Alpha Lipoic Acid on Polychlorinated Biphenyl (Aroclor 1254)-Induced Nephrotoxicity in Rats

Abstract

The present study aimed to determine the morphological changes in rat kidneys exposed to PCB (Aroclor 1254) and to reveal the effects of ALA on those changes. Rats were randomly divided into four groups: Control, ALA, PCB and ALA+PCB. The applications of ALA and PCB continued for thirty days. At the end of the experiment, the tissue samples were taken from the animals. Crossmon’s triple staining method was applied to kidney sections for histopathological and histomorphometric examinations. In addition, the periodic acid-Schiff (PAS) reaction was examined in tissue sections. Histopathologically, it was determined that glomerular degeneration and connective tissue densities in the kidney significantly increased in PCB group compared to Control group. Besides, it was found that glomerular degeneration intensity significantly decreased in ALA+PCB group compared to PCB group. It was detected that the diameters of the renal corpuscle, proximal tubule, ascending limb of Henle’s loop and collecting duct in the kidney significantly decreased in PCB group compared to Control group. However, these parameters significantly increased in ALA+PCB group compared to PCB group. While the density of glomerular mesangial matrix and glycogen density in proximal tubule epithelium significantly increased in PCB group compared to Control group, these parameters significantly decreased in ALA+PCB group compared to PCB group. As a result, PCB may adversely affect renal functions by causing disorders in kidney histology. In addition, the use of ALA against PCB exposure may contribute to the preservation of kidney tissue.

Keywords

Alpha lipoic acid, Aroclor 1254, Histology, Kidney, Rat

Ethical Statement

This study was carried out at Aydin Adnan Menderes University Experiment Animals Application Center. The study was approved by Aydin Adnan Menderes University Animal Experiments Local Ethics Committee (ADUHADYEK Ref No: 6458310/2021/180).

Thanks

I would like to thank Prof. Dr. Ulker Eren and Associate Prof. Ozay Gules for their guidance and support.

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