Hesperidin Mikroemülsiyonunun Staphylococcus aureus ve Listeria monocytogenes'e Karşı In Vitro Antibakteriyel Etkisi

Abstract

Gıda kaynaklı bakteriyel enfeksiyonlar ve zehirlenmeler dünya genelinde bakteriyel hastalıkların büyük bir bölümünü oluşturmaktadır. Son yıllarda bu hastalıkların tedavisinde kullanılan kemoterapötiklere karşı artan bir direnç söz konusudur. Yapılan çalışmalar, doğal ürünlerden ve bitkilerden elde edilen bazı bileşiklerin antibiyotiklere alternatif potansiyele sahip olduğunu göstermiştir. Bu çalışmada hesperidin mikroemülsiyonunun Listeria monocytogenes ve Staphylococcus aureus üzerindeki antimikrobiyal potansiyelinin ortaya konulması amaçlanmıştır. Çalışmada, in vitro antimikrobiyal aktiviteyi göstermek için broth mikrodilüsyon yöntemi kullanılmıştır. Mikrodilüsyon testinde hesperidinin S. aureus ve L. monocytogenes için MİK değerleri 128 μg/mL olarak bulunmuştur. Buna karşılık, ampisilinin S. aureus ve L. monocytogenes için MİK değerleri 0.5 μg/mL olarak bulunmuştur. Hesperidin biyolojik olarak değerli özelliklere sahip bir bileşiktir. Antimikrobiyal etkisi antibiyotiklere alternatif olarak umut verici bir alan açabilir. Bununla birlikte, terapötik potansiyelini tam olarak anlamak ve etki mekanizmalarını tanımlamak için hesperidin ve benzeri potansiyel aktif maddeler üzerinde ayrıntılı çalışmalara ihtiyaç vardır.

Keywords

• Hesperidin
• Antibakteriyel
• Staphylococcus aureus
• Listeria monocytogenes
• MIC

In Vitro Antibacterial Effect of Hesperidin Microemulsion to Staphylococcus aureus and Listeria monocytogenes

Abstract

Foodborne bacterial infections and intoxications constitute a large proportion of bacterial diseases worldwide. In recent years, there has been an increasing resistance to chemotherapeutics used in the treatment of these diseases. Studies have shown that some compounds extracted from natural products and plants have alternative potential to antibiotics. In this study, it was aimed to reveal the antimicrobial potential of hesperidin microemulsion on Listeria monocytogenes and Staphylococcus aureus. In the study, the broth microdilution method was used to demonstrate in vitro antimicrobial activity. In the microdilution test, the MIC values of hesperidin for S. aureus and L. monocytogenes were as 128 μg/mL. In comparison, the MIC values of ampicillin for S. aureus and L. monocytogenes were as 0.5 μg/mL. Hesperidin is a compound with biologically valuable properties. Its antimicrobial effect may open a promising field as an alternative to antibiotics. However, detailed studies on hesperidin and similar potential active substances are needed to fully understand its therapeutic potential and to define its mechanisms of action.

Keywords

• Antibacterial
• Staphylococcus aureus
• Listeria monocytogenes
• MIC
• Hesperidin

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