Detection of The Presence of Leptospirosis in Horses by ELISA Method

Abstract

Leptospirosis is one of the common zoonotic diseases worldwide. It significantly affects humans and animals, causing socio-economic losses. Equine leptospirosis often manifests itself with recurrent genital tract infections, reproductive disorders, abortion, embryonic death, and birth of weak foals. Studies on Leptospirosis, especially in horses, are quite limited. For this reason, our study was planned to investigate the transmission cycle in terms of farm animals and public health, and also to determine the presence of Leptospirosis in horses. For our research, 187 horse blood serum samples were collected from different settlements in Turkey. In the collected samples, Leptospira spp. ELISA test was used to investigate the presence of antibodies. A total of 8 samples (4.27%) were detected as seropositive in horse serum samples. The agent, a significant pathogen for both human and animal health, was identified as seropositive. In addition, the high rate of transmission of leptospirosis through contact with infected horses and the potential for spread through different animals should be taken into consideration. Leptospirosis was determined as seropositivity by ELISA in the horses sampled. Therefore, it is important to diagnose and control the infection rapidly.

Keywords

• ELISA
• Horse
• Leptosipirosis
• Serologic Test
• Zoonoses

Atlarda Leptospirosis Varlığının ELISA Yöntemi ile Tespiti

Abstract

Leptospirosis dünya çapında yaygın zoonotik hastalıklardan biridir. İnsanları ve hayvanları önemli ölçüde etkileyerek sosyo-ekonomik kayıplara neden olur. At leptospirozu sıklıkla tekrarlayan genital sistem enfeksiyonları, üreme bozuklukları, abort, embriyonik ölüm ve zayıf tayların doğumu ile kendini gösterir. Özellikle atlarda Leptospirozis üzerine yapılan çalışmalar oldukça kısıtlıdır. Bu sebeple, çiftlik hayvanları ve halk sağlığı açısından bulaşma döngüsünü araştırmak, aynı zamanda atlarda Leptospirozis’in varlığını belirlemek amacıyla çalışmamız planlanmıştır. Araştırmamız için Türkiye’nin farklı yerleşim yerlerinden 187 adet at kan serumu örneği toplandı. Toplanan örneklerde, Leptospira spp. antikorlarının varlığını araştırmak için ELISA testi kullanıldı. At serum örneklerinde toplam 8 örnek (%4,27) seropozitif olarak tespit edildi. İnsan ve hayvan sağlığı için önemli bir patojen olan etken seropozitif olarak teşhis edilmiştir. Ayrıca, leptospirozis hastalığının enfekte atlarla temas yoluyla bulaşma oranının yüksek olması ve farklı hayvanlar ile yayılma potansiyeli göz önüne alınmalıdır. Örnek alınan atlarda Leptospirosis ELISA ile seropoziti olarak belirlenmiştir. Bu nedenle enfeksiyonun hızla teşhis edilerek kontrol altına alınması önem taşımaktadır.

Keywords

• Serolojik Test
• At
• ELISA
• Leptospirozis
• Zoonozlar

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