Kocatepe Gebe Düvelerde İnaktif Parapoxvirus Ovis Uygulamasinin Kolostrum ve Buzaği Sağliği Üzerine İmmunmodulatorik Etkileri

Öz

Sunulan bu çalışmanın amacı sığırlarda gebeliğin son döneminde uygulanan inaktif Parapoxvirus Ovis (İPPVO) immunmodulasyon protokolünün kolostrum kalitesi, pasif transfer başarısı ve buzağı sağlığı üzerine etkilerinin araştırılmasıdır. Çalışmanın hayvan materyalini 20 kontrol ve 20 İPPVO grubu olmak üzere 40 adet Holstein ırkı gebe düve ve buzağıları oluşturdu. İPPVO grubuna muhtemel doğum tarihlerine 25, 23 ve 21 gün kala, toplamda 3 doz, 2 ml lik İPPVO preparatı, intramusküler (Zylexis, Zoetis, USA), kontrol grubuna ise aynı planda ve hacimde izotonik NaCI %0.9 enjeksiyonu yapılmıştır. Doğumla birlikte annelerden kolostrum örnekleri, buzağılardan ise doğumdan 24 – 48 saat sonra kan alınarak serumları çıkartılmıştır. Kolostrum ve serum örneklerinde ELISA yöntemi kullanılarak IgG konsantrasyonları ölçülmüştür. Buzağılar ilk 30 günlük dönemde sağlık yönünden takip edilmiştir. Çalışma kapsamında İPPVO preperatı kullanılan düvelerde herhangi bir yan etki gözlenmemiştir. İPPVO uygulanan düvelerin kolostrum IgG konsantrasyonları 59.64 mg/ml, kontrol grubunda ise 59.62 mg/ml olarak ölçülmüş ve aradaki fark istatistik yönden anlamsız bulunmuştur (p>0.05). İPPVO uygulanan düvelerden doğan buzağıların ortalama kan serumu IgG konsantrasyonu 32.6 mg/ml, kontrol grubunda 28.7 mg/ml olarak ölçülmüştür. Çalışma kapsamında iki grup buzağıları arasında yapılan günlük sağlık kontrolleri sonucunda toplanılan veriler değerlendirildiğinde hastalığa yakalanma ve buzağı ölümleri yönünden de anlamlı bir farklılık tespit edilmemiştir (p>0.05).

Anahtar Kelimeler

• Gebe Holstein Düve
• IgG
• İmmunmodülasyon
• İnaktif Parapoxvirus Ovis
• Kolostrogenezis

Immunomodulatory Effects of Inactive Parapoxvirus Ovis Administration to Pregnant Heifers on Colostrum and Calf Health

Abstract

The aim of this study was to evaluate the effect of inactive Parapoxvirus ovis (IPPVO) immunomodulation protocol administration to pregnant heifers in the third trimester of pregnancy on colostrum quality, passive transfer success and calf health. Animal material of the study included 40 Holstein breed pregnant heifers and their calves, 20 of them was IPPVO group and rest 20 was control group. Totally 3 doses of 2 ml IPPVO (Zylexis, Zoetis, USA) was administered intramuscularly to study group in 25, 23 and 21 days before estimated birth date. Also control group was injected the same amount of isotonic NaCI %0.9 with IPPVO in the same days. Colostrum samples from heifers after birth and blood samples from calves 24-48 hours after birth were taken and sera were extracted. Immunoglobulin G (Ig G) concentrations of colostrum and serum samples were measured by using enzyme linked immunoassay (ELISA) method. Calves’ health status were observed during first 30 days. There was no adverse effect was detected in heifers administered IPPVO during study procedure. IgG concentrations of heifers that received IPPVO was 59.64 mg/ml; that of control group was 59.62 mg/ml and the difference between groups was statistically insignificant (p>0.05). Average serum IgG concentration of calves born from heifers that received IPPVO was 32.6 mg/ml; that of control group was 28.7 mg/ml. When the yielded results of daily health status controls of two claves groups evaluated, no statistically significant difference was detected in terms of developing any disease and calf deaths (p>0.05).

Keywords

• Colostrogenesis
• Holstein Pregnant Heifer
• IgG
• Immunomodulation
• Inactive Parapoxvirus Ovis

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