Comparison of Allele Frequencies of LAP3 and MC4R Gene Polymorphisms in Berrichon Du Cher, Ile De France, and Karacabey Merino Sheep

Abstract

Meat production yield and quality are common objectives for the Berrichon Du Cher (BDC), Ile De France (IDF), and Karacabey Merino (KM) sheep, which are bred for slaughter. Growth performance has a crucial role in these traits. LAP3 and MC4R genes are thought to influence growth and development in sheep by affecting embryonic cell functions, adult height, or body weights at different ages. The polymorphisms of the LAP3 and MC4R genes have been studied in cattle and razor clams, but only to a limited extent in in sheep breeds. Moreover, the novel SNP of target genes have not been studied in meat sheep such as BDC, IDF, and KM. Thus, the aim of this study is to compare the allele and genotype frequencies of LAP3 and MC4R genes in BDC, IDF, and KM sheep. The BDC (n=48), IDF (n=58), KM(n=54) sheep were genotyped by PCR- RFLP method. Three different breeds of animals were compared for the LAP3 gene; differences between groups were assessed using chi-square test. According to the results, the genotype frequencies for g.232C>G of the LAP3 gene were calculated as 54.17% and 45.83% for GC and GG, respectively, in BDC. Similarly, the GC (57.41%) and GG (42.59%) genotypes were detected in KM. However, only one genotype (GG) was found in IDF for the LAP3 gene. Similarly, all investigated flocks were identified as GG in terms of the MC4R gene. As a consequence, these findings could be a source for further projects. Expanding the study by using different gene pools in terms of the investigated races should be useful for identifying the genetic structure of the flocks in detailed.

Keywords

• Berrichon Du Cher
• Ile De France
• Karacabey Merino
• LAP3
• MC4R

Ethical Statement

This research was approved by the Animal Experiments Local Ethics Committee of Uludag University (Approval number: 2021-01/03).

Thanks

We would like to express our gratitude to ACE Hayvancılık A.Ş. for its contribution to the study in terms of providing the study material.

Berrichon Du Cher, Ile De France ve Karacabey Merinosu Koyunlarında LAP3 ve MC4R Gen Polimorfizmlerine İlişkin Allel Frekanslarının Karşılaştırılması

Abstract

Et üretimi ile birlikte verim ve kalite, besi amaçlı yetiştirilen Berrichon Du Cher (BDC), Ile De France (IDF) ve Karacabey Merinosu (KM) koyunları için ortak bir hedeftir. Büyüme performansı, bu özellikte kritik bir rol oynamaktadır. LAP3 ve MC4R genlerinin, embriyonik hücre fonksiyonlarını, yetişkinlikteki boy uzunluğunu veya farklı yaşlardaki vücut ağırlıklarını etkileyerek koyunlarda büyüme ve gelişimi etkilediği düşünülmektedir. LAP3 ve MC4R gen polimorfizmleri, sığır ve denizçakısı (ustura istiridye) türlerinde araştırılmış olup, koyun ırklarında bu araştırmalar sınırlı kalmıştır. Ayrıca, bu genlerin yeni SNP’leri etçi koyunlar olan BDC, IDF ve KM’de henüz çalışılmamıştır. Bu nedenle, çalışmanın amacı, BDC, IDF ve KM koyunlarında LAP3 ve MC4R genlerinin allel ve genotip frekanslarını karşılaştırmaktır. BDC (n=48), IDF (n=58) ve KM (n=54) genotiplendirmesi PCR-RFLP yöntemiyle yapılmıştır. Üç farklı ırk hayvan, LAP3 geni açısından karşılaştırıldı; gruplar arasındaki farklar ki-kare kullanılarak değerlendirildi. Elde edilen bulgulara göre; LAP3 geninin g.232C>G polimorfizmi için BDC’de genotip frekansları GC ve GG genotipleri sırasıyla % 54,17 ve % 45,83 olarak hesaplanmıştır. Benzer şekilde, KM’de GC (% 57,41) ve GG (% 42,59) genotipleri tespit edilmiştir. Ancak, IDF koyunlarında sadece bir genotip (GG) belirlenmiştir. Benzer şekilde, incelenen tüm ırklar MC4R geni açısından GG genotipi ile tanımlanmıştır. Sonuç olarak, elde edilen sonuçların ilerideki çalışmalara temel oluşturacağı düşünülmektedir. Araştırmanın, incelenen ırklar açısından farklı gen havuzları kullanılarak genişletilmesi, sürülerin genetik yapısının daha ayrıntılı belirlenmesi açısından faydalı olacaktır.

Keywords

• Berrichon Du Cher
• Ile De France
• Karacabey Merinosu
• LAP3
• MC4R

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