Morin Reduces Ciprofloxacin-Induced Brain Damage by Regulating Oxidative Stress, Inflammation, and Histopathological Changes

Abstract

Ciprofloxacin (CPFX), one of the fluoroquinones, is an antibacterial antibiotic that is used in the treatment of urinary tract, respiratory, abdominal and gastrointestinal infections as well as having negative side effects on the central nervous system. Morin, a bioactive flavonoid, is an antioxidant with neuroprotective properties thanks to its many pharmacological properties. The protective effect off Morin against brain damage caused by CPFX, broad spectrum antibiotic, was investigated. Twentyeight female rats were divided into four groups as control, Morin, CPFX, CPFX+Morin. CPFX, Morin was administered once a day for seven days. Oxidative stress and inflammation parameters were analyzed to determine brain tissue damage and histopathological analysis was performed to determine tissue damage and structural changes. According to the findings obtained as a result of the analyses, CPFX increased nuclear factor kappa B (NF-B), malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), and interleukin 1β (IL-1β) and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) in brain. Co-administration of CPFX with Morin increased GSH and GPx, SOD, CAT and decreased NF-B, MDA, TNF-α, IL-1β levels. According to findings, when evaluated together, it was found that CPFX caused damage in brain tissue by causing oxidative stress and inflammation and Morin as a supportive treatment reduced the damage in brain tissue by bringing these markers closer to normal.

Keywords

• Brain
• Ciprofloxacin
• Inflammation
• Morin
• Oxidative stress

Morin, Oksidatif Stresi, İnflamasyonu ve Histopatolojik Değişiklikleri Düzenleyerek Siprofloksasin Kaynaklı Beyin Hasarını Azaltır

Abstract

Florokinonlardan olan siprofloksasin (CPFX), idrar yolu, solunum, abdominal ve gastrointestinal enfeksiyonların tedavisinde kullanılmasının yanı sıra merkezi sinir sistemi üzerinde olumsuz yan etkileri bulunan antibakteriyel antibiyotiktir. Biyoaktif flavonoid olan Morin, birçok farmakolojik özellikleri sayesinde nöroprotektif özelliğe sahip antioksidandır. Geniş spektrumlu antibiyotik olan CPFX’in neden olduğu beyin hasarına karşı Morin’in koruyucu etkisi araştırıldı. Bu amaçla yapılan çalışmada yirmisekiz adet dişi rat kontrol, Morin, CPFX, CPFX+Morin olarak dört gruba ayrıldı. CPFX, Morin yedi gün boyunca günde bir defa uygulandı. Beyin dokusunda hasarı belirlemek amacıyla oksidatif stres ve inflamasyon parametleri ve doku hasarını ve yapısal değişiklikleri belirlemek için histopatolojik analiz yapıldı. Analizler sonucunda elde edilen bulgulara göre CPFX, beyinde nuclear factor kappa B (NF-B), malondialdehit (MDA), tumor necrosis factor-alpha (TNF-α) ve interleukin 1β (IL-1β)’i artırdığı, glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD), katalaz (CAT) ile glutatyon (GSH)’yı azalttığı bulundu. CPFX’in Morin ile birlikte uygulanmasının GSH ile GPx, SOD, CAT’i artırdığı, NF-B, MDA, TNF-α, IL-1β düzeylerini azalttığı tespit edildi. Elde edilen bulgulara göre birlikte değerlendirildiğinde, CPFX’in oksidatif stres ve inflamasyona neden olarak beyin dokusunda hasara neden olduğu ve destekleyici tedavi olarak Morin’in bu belirteçleri normale yaklaştırarak beyin dokusunda hasarı azalttığı bulundu.

Keywords

• Beyin
• İnflamasyon
• Morin
• Oksidatif stres
• Siprofloksasin

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