Macroanatomical, Subgross and Stereological Investigation of The Nerve Nodes in The Buffalo Heart

Abstract

Sinoatrial (SA) and Atrioventricular (AV) nodes are responsible for the nerve conduction of the hearth, that provides electricial signals for normal cardiac rhythm. In this study we investigated the nevre nodes of the water buffalos by means of the stereological methods. SA node and AV node from the water buffalo heart obtained from slaughter house were dissected and fixed into the 10 % neutral formaldehyte sollution. After the histological processing of the tissue, we investigated the serial sections in stereologically. The pale stained nodal areas that include smaller fibers and perinuclear cells were identified in sinoatrial nodes. The dimensions of the sinoatrial node were ~18 mm x 2.9 mm x 2 mm and the volume was 122.83 mm3. The atrioventricular node located just under the endocardium of the interatrial septum, at the level of valva tricuspitalis and above the os cordis. The dimensions of the atrioventriculer node were ~9 mm x 4.3 mm x 3.1 mm and the volume was 119.03 mm3.

Keywords

• Buffalo,Nodus atrioventricularis,Nodus sinoatrialis,Morphology,Stereology

Manda Kalbindeki Sinir Düğümlerinin Makroanatomik, Subgross ve Stereolojik İncelenmesi

Abstract

Kalbin sinir düğümleri (nodus sinoatrialis ve nodus atrioventricularis) kendine has olan iletim sisteminin bir parçası olarak görev yapan birimler olup, kalbin normal ritminde çalışması için gerekli elektriksel iletimleri sağlar. Bu çalışmamızda yöresel olarak öneme sahip olan mandaların kalbinde sinir düğümlerini stereolojik metodlar kullanarak inceledik. Mezbahadan temin ettiğimiz manda kalplerinde öncelikle sinir düğümlerinin olduğu bölgeler diseke edildi ve %10’luk nötral formaldehit çözeltisinde tespit edildi. Doku takibi ve parafine gömme aşamalarının ardından sinoatriyal düğüm için ~1.7 mm, atriyoventriküler düğüm için ~0.9 mm aralıklar bırakılarak 5 μm kalınlığında kesitler alındı ve bu kesitlerde stereolojik olarak hesaplamalar yapıldı. İncelemelerimiz sonucu sinoatriyal düğümlerde daha soluk boyanan, daha küçük çapta lifler taşıyan ve perinüklear bölge hücreleri içeren düğüm bölgelerini tespit ettik ve ölçümlerimiz sonucu sinoatriyal düğüm boyutunu ~18mm x 2.9mm x 2mm, hacmini ise 122.83 mm3 olarak tespit ettik. Atriyoventriküler düğümün ise atrium dextrum’un duvarında endocardium’un hemen altında triküspit kapakçıklar hizasında ve os cordis’in hemen üzerinde olduğunu, bölge koroner arterlerinin ramus interventricularis’lerden çıkarak interatrial bölgeye giden damarlarla kanlandığını ve yine bu bölgede purkinje sinir hücrelerinin varlığını tespit ettik. Ölçümlerimiz sonucu atrioventriculer düğüm boyutunu ~9 mm x 4.3 mm x 3.1 mm, hacmini ise 119.03 mm3 olarak tespit ettik.

Keywords

• Nodus atrioventricularis,Nodus ainoatrialis,Manda,Morfoloji,Stereoloji

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