The Effects of Amoxicillin Clavulanic Acid, Amoxicillin Clavulanic Acid + Vitamin Administration on Malondialdehyde and Antioxidant Levels in Kidney and Liver Tissues of the Rats

Abstract

It is aimed to investigate the effects of Amoxicillin Clavulanic Acid (AKA) and vitamins on antioxidants and oxidative stress in liver and kidney tissues of the rats. 49 Wistar-albino male rats were divided into seven groups. We applied; group1: AKA, group2: AKA+vitamin C, group3: AKA+vitamin E, group4: AKA+vitamin C+vitamin E, group5: vitamin C, group6: vitamin E and group7: as a control group. At the end of the study Malondialdehyde(MDA), reduced glutathione(GSH), catalase activities(CAT), vitaminC, total protein levels in liver and kidney tissues of the rats were analyzed, and plasma ALT, AST and GGT levels were measured. The ALT increased significantly in Group1(p<0.001). Renal tissue MDA increased in only group1(p<0.05). A significant decrease was determined in GSH levels in group2 and 4(p<0,05). CAT showed a significant increase in group1(p<0.01) and a decrease in group4(p<0.05). Group6 showed an increase in CAT activity(p<0.01). Renal tissue vitaminC was significantly lower in group1(p<0.001). Liver tissue MDA were increased in all groups(1., 3. and 5. groups p<0.05; 2., 4. and 6. groups p<0.01). GSH decreased in only group4(p<0.01). CAT activities decreased significantly in all other groups (group1 and 2; p<0.01, group6; p<0.05). VitaminC decreased in group1(p<0.001) and group2(p<0.01) significantly. Total protein in liver tissue decreased only in group4(p<0.001). Changes in parameters suggest that there may be oxidative damage-induced degenerations in liver and kidney cells. As a result, it has come to the conclusion that it may be useful to use antioxidants in addition to antibiotics against oxidative damage.

Keywords

• Amoxicillin clavulanic acid
• Antioxidants
• Malondialdehyde
• Rat
• Vitamin

Amoksisilin Klavulanik Asit, Amoksisilin Klavulanik Asit+Vitamin Uygulamasının Ratların Böbrek ve Karaciğer Dokularındaki Malondialdehit ve Antioksidan Düzeylerine Etkisi

Abstract

Bu çalışmayla amoksisilin klavulanik asitin (AKA) ve vitaminlerin, sıçanların karaciğer ve böbrek dokusunda antioksidanlar ve oksidatif stres üzerine etkilerini incelemek amaçlandı. 49 Wistar–albino erkek sıçan; 1. grup: AKA, 2. grup: AKA+vitamin C, 3. grup: AKA+vitamin E, 4. grup: AKA+vitamin C+vitamin E, 5. grup: vitamin C, 6. grup: vitamin E, 7. grup: kontrol grubu olarak ayrıldı. Çalışma sonunda karaciğer ve böbrek dokusunda malondialdehit (MDA), redükte glutatyon (GSH), katalaz aktivitesi (CAT), vitamin C, total protein, plazma ALT, AST ve GGT miktarları ölçüldü. 1. grupta ALT önemli düzeyde arttı (p<0.001). Böbrek dokusu MDA sadece 1. grupta artış gösterdi (p<0.05). Böbrek dokusu GSH düzeylerinde 2. ve 4. grupta önemli miktarda azalma saptanmıştır (p<0.05). Böbrek dokusu CAT aktivitesi 1. grupta önemli bir artış (p<0.01), 4. grupta ise azalma gösterdi (p<0.01). 6. grupta CAT aktivitesinde artış gözlendi (p<0.05). Böbrek dokusu vitamin C 1. grupta önemli derecede düşük bulundu (p<0.001). Karaciğer dokusu MDA grupların tamamında artış göstermiştir ( 1., 3. ve 5. grup, p<0.05; 2., 4. ve 6. grup, p<0.01). GSH sadece 4. grupta azaldı (p<0.01). CAT aktiviteleri diğer bütün gruplarda önemli düzeyde azaldı (1. 2. grupta (p<0.01), 6. grupta(p<0.05). VitaminC 1.(p<0.001) ve 2. grupta (p<0.01) önemli derecede azalmıştır. Total protein karaciğer dokusunda sadece 4. Grupta (p<0.001) azalmıştır. Parametrelerdeki değişimler karaciğer ve böbrek hücrelerinde oksidatif hasardan kaynaklı dejenerasyonların olabileceğini göstermektedir. Sonuç olarak, oksidatif hasarlara karşı antibiyotiğe ek olarak antioksidanların da kullanılmasının faydalı olabileceği kanısına varılmıştır. 

Keywords

• Amoksisilin klavulanik asit
• Antioksidanlar
• Malondialdehit
• Sıçan
• Vitamin

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