Sisteamin ve Alfa Lipoik Asit Kombinasyonunun Dondurulmuş-Çözdürülmüş Boğa Sperması Parametreleri Üzerine Etkisi

Öz

Sunulan çalışmada sisteamin, trehaloz, alfa-lipoik asit ve bu antioksidan kombinasyonlarının, çözüm sonu boğa spermasında spermatolojik ve oksidatif stres parametreleri üzerine koruyucu etkinliklerinin belirlenmesi amaçlandı. Beş adet sağlıklı holştayn (3-4 yaşlarında) ırkı boğa kullanıldı. Çalışmada kullanılan her boğadan 8 ejakülat alındı. Alınan ejakülatlar miks yapılarak 37 °C’de 7 eşit hacme bölündükten sonra, sisteamin 2 mM, trehaloz 50 mM, alfa-lipoik asit (ALA) 1 mM, sisteamin 2 mM + trehaloz 50 mM, ALA 1 mM + trehaloz 50 mM, sisteamin 2 mM + ALA 1 mM ve antioksidan içermeyen (kontrol) temel sulandırıcı ile sulandırılarak 5 °C’de soğutulmasının ardından donduruldu. Dondurulan payetler su banyosunda çözdürülerek değerlendirildi. Sisteamin 2 mM + ALA 1 mM kombinasyonunu içeren sperma sulandırıcısının çözüm sonu subjektif (% 68 ± 2.7) ve progresif motilite (% 42.9 ± 4.7) oranları üzerine kontrol gruplarına kıyasla (% 61 ± 4.2 ve % 37.5 ± 8) olumlu etkinliği gözlenirken istatiksel olarak fark önemsiz bulundu (P> 0.05). Sisteamin 2 mM + ALA 1 mM kombinasyonu içeren sperma sulandırıcı grubu akrozom bütünlüğü ve mitokondriyal aktivite oranları (% 52.02 ± 6.4 ve % 32 ± 4.1) kontrol gruplarına (% 30.5 ± 1.7 ve % 14.02 ± 3.5) göre istatistiksel olarak daha yüksek bulundu (P < 0.05). Dondurulmuş çözdürülmüş boğa sperma sulandırıcılarına eklenen sisteamin ve ALA kombinasyonu spermatozoon motilitesine, akrozom bütünlüğüne ve mitokondriyal aktivite bütünlüğüne katkı sağladı.

Anahtar Kelimeler

• Alfa Lipoik Asit,Boğa Sperması,Sisteamin,Floresan Boyama,Trehaloz

Combination of Cysteamine and Lipoic Acid Improves the Post-Thawed Bull Sperm Parameters

Abstract

The present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significantly, P>0.05). The supplementation of the semen extender with combination of cysteamine 2 mM and ALA 1 mM produced a higher acrosome integrity and mitochondrial activity (52.02 ± 6.4% and 32 ± 4.1%, respectively), compared with the controls (30.5 ± 1.7 and 14.02 ± 3.5% respectively, P < 0.05). Combination of cysteamine and ALA antioxidants in semen extenders provided the benefit in terms of sperm motilities, acrosome integrity and mitochondrial activity on frozen-thawed bull sperm

Keywords

• Alpha Lipoic Acid,Bull Sperm,Cysteamine,Fluorescent Staining,Trehalose

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