Yerli Kara Düvelerde Süperovulasyon ve Embriyo Verimi Üzerine Farklı FSH Uygulamalarının Etkileri

Abstract

Bu çalışmanın amacı Yerli Kara düvelerde süperovulasyon oluşturma ve embriyo elde etme yönünde iki farklı follikül uyarıcı hormonunun etkinliğini gözlemlemekti. Düvelere 1.56 g progesteron içeren Cue-mate uygulandı. Düveler dört gruba ayrıldı. Folltropin (F) grubuna control olarak 400 mg folltropin diğer taraftan Ovagen (O) grubuna ise sırasıyla 8.8 mg (O1), 11.4 (O2) ve 17.6 (O3) olmak üzere Ovagen uygulandı. Süperovulasyon protokolünün 9. gününde Cue-mate çıkarıldı ve gruplardaki tüm ineklere 500 μg prostaglandinF2α yapıldı. 11. gün akşam ve 12. gün sabah olmak üzere 12 saat ara ile tüm ineklere Yerli Kara boğalardan alınan spermalar ile suni tohumlama yapıldı. Suni tohumlama uygulamasından 7 gün sonra embriyo elde etme çalışması ile embriyolar toplandı. Ortalama CL sayıları F grubunda 6.33 ± 0.718 belirlenirken, O grubunda uygulanan üç farklı dozdan (sırasıyla 3.82 ± 0.502, 3.50 ± 0.513, 3.58 ± 0.448; p<0.001) elde edilen CL sayılarından daha yüksek bulundu. Transfer edilebilir embriyo oranları uygulama grupları arasında farklı değildi (p>0.05). Sonuç olarak, bulgular F grubunda yapılan uygulamanın CL, elde edilen embriyo ve ovum sayısını artırmasına rağmen transfer edilebilir embriyo sayısına olumlu bir etkisinin olmadığını gösterdi.

Keywords

• Yerli Kara Düve,Folltropin,FSH,Ovagen,Süperovulasyon

Various FSH Administration on Superovulatory Response and Embryo Yield in Anatolian Black Heifers

Abstract

The aim of the present study was to investigate effectiveness of superovulatory response and embryo yield in Anatolian Black heifers, induced with the administration of two different follicle stimulating hormone. Heifers received a progesterone releasing device (Cue-mate containing 1.56 g progesterone). The heifers were randomly assigned to four groups. Group Folltropin (F) were administered with Folltropin (400 mg) as a control, while those included in Groups Ovagen (O) were administered with Ovagen at doses of 8.8 mg (O1), 11.44 mg (O2) and 17.6 mg (O3) respectively. On the 9th day of the application, the Cue-mate was removed and the heifers received 500 μg prostaglandinF2α in all goups. The heifers were artificially inseminated (AI) using semen obtained from Anatolian Black bulls; in the evening of day 11 and in the morning of day 12 with 12 hours intervals. Embryos were collected by uterine flushing 7 days after AI. The mean number of CL determined as 6.33±0.718 in F, was found to be higher than the numbers obtained with the administration of the three different doses of O (3.82±0.502, 3.50±0.513, 3.58±0.448 respectively; p<0.001). The transferable embryo yield did not differ significantly among the treatment groups (p>0.05). In conclusion, findings show that although the administration of F was ascertained increase the number of CL and the total number of ova/embryos recovered, these increased numbers had no reflection on the number of transferable embryos in Anatolian Black heifers.

Keywords

• Anatolian Black heifer,Folltropin,FSH,Ovagen,Superovulation

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