Isothermal Amplification Methods Most Used in the Diagnosis of Infections

Abstract

Isothermal amplification of nucleic acid is one of the most commonly used methods to identify the infections, tumors, genetic abnormalities, and in forensic medicine. As isothermal amplification methods can be performed at one reaction temperature under simple conditions they are less complex in comparison to Polymerase Chain Reaction (PCR). These methods differ from each other with the diversity of enzymes, primers used, sensitivity and specificity. In this review, the most commonly used isothermal amplification methods, their principles, areas of use and innovations brought about are discussed.

Keywords

• Isothermal Amplification,LAMP,NASBA,SDA,EXPAR

İnfeksiyonların Tanısında En Çok Kullanılan İzotermal Amplifikasyon Yöntemleri

Abstract

Nükleik asit amplifikasyonu, enfeksiyonların, tümörlerin, genetik anormalliklerin tanısında ve adli tıpta en fazla kullanılan yöntemlerdendir. İzotermal amplifikasyon yöntemleri, Polimeraz Zincir Reaksiyonu (PZR) metoduna göre sabit sıcaklıkta yapılmasıyla karmaşıklığı azaltır. Bu yöntemler, kullanılan enzim çeşitliliği, primerler, duyarlılıkları ile özgüllükleri bakımından farklılıklar göstermektedir. Bu derlemede izotermal amplifikasyon yöntemlerinden en sık kullanılanları, prensipleri, kullanım alanları ve getirdikleri yenilikler ele alınmıştır.

Keywords

• İzotermal Amplifikasyon,LAMP,NASBA,SDA,EXPAR

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