Effects of Vitamin C on Male New Zealand White Rabbits Exposed to Endosulfan

Year 2018, , 165 - 172, 18.04.2018

Özlem Yıldız_gülay Tülay Büyükoğlu , Fatma Şefika Hatipoğlu , Mehmet Şükrü Gülay

https://doi.org/10.30607/kvj.394088

Abstract

The present study evaluated the protective
effect of oral Vitamin C (VitC) against changes in hematological and
biochemical parameters, and oxidant-antioxidant status in male New Zealand
White rabbits treated with endosulfan. Total of 24 rabbits (6 to 8 years old)
were randomly divided into 4 treatment groups (n=6). Rabbits in the control
group (TRT-I) received corn oil for 6 weeks. Rabbits in the second group
(TRT-II) received endosulfan (1 mg/kg bw per day) in corn oil. Rabbits in the
third group (TRT-III) received corn oil daily and VitC (20 mg/ kg bw) every
other day for 6 weeks. Rabbits in the last group (TRT-IV) received the same
amounts of endosulfan and VitC. All treatments were administered by oral route.
Although total erythrocyte, trombocyte, hemoglobin and percent hematocrit,
lymphocyte, granulocyte and monocyte did not differ among the treatment groups,
total leukocyte numbers differed due to treatment. The serum glucose, alanine
aminotransferase (ALT), malondialdehyde (MDA), glucose 6 phosphate
dehydrogenase (G6PD), glutathion peroxidase (GPx), and erythrocyte catalase
(CAT) in treatment groups were similar. However, serum VitC levels were
elevated for rabbits in TRT-II  group. In
addition, serum alkaline phosphatase (ALP) and aspartate aminotransferase (AST)
levels increased in the TRT-II group, whereas VitC application decreased ALP
and AST levels in the TRT-IV group when compared to the TRT-II group. Thus,
results suggested beneficial influences of VitC in neutralizing some harmful
effects of endosulfan in male New Zealand White rabbits.

Keywords

blood parameters, biochemical parameters, oxidant-antioxidant status, endosulfan toxication, insecticide use, Vitamin C

Endosülfan ve C Vitamini Uygulamalarının Erkek Yeni Zellanda Tavşanları Üzerindeki Etkisi

Abstract

Çalışmamızda
endosülfan uygulaması yapılan erkek Yeni Zellanda tavşanlarında C vitamini
(VitC) ilavesinin bazı kan ve biyokimyasal parametreler ile oksidan-antioksidan
denge üzerine düzeltici etkisinin araştırılması amaçlanmıştır. Bu amaçla,
yaşları 6 ile 8 ay arasında değişen 24 tavşan, her grupta 6 tavşan olacak
şekilde, rastgele 4 çalışma grubuna ayrıldı. Kontrol grubundaki tavşanlara
(TRT-I) mısır yağı verildi. İkinci grupta bulunan tavşanlara (TRT-II) günlük 1
mg/kg endosülfan mısır yağında eritilerek verildi. Üçüncü gruptaki
tavşanlara  20 mg/kg dozunda VitC gün
aşırı olarak uygulandı (TRT-III). Son gruptaki tavşanlara (TRT-IV) aynı
oranlarda endosülfan ve VitC verildi. Oral yolla yapılan bu uygulamalar 6 hafta
boyunca devam etti. Çalışmamızda toplam eritrosit ve trombosit, hemoglobin,
hematokrit, ve lökosit değerleri yönünden gruplar arasında fark gözlenmezken,
toplam lökosit sayılarında anlamlı bir fark bulundu. Ayrıca serum glukoz,
alanin aminotransferaz (ALT), malondialdehit (MDA), glukoz 6 fosfat
dehidrojenaz (G6PD), glutatyon peroksidaz (GPx) ve eritrosit katalaz (CAT)  aktivitelerinde de farklılık tespit edilmedi.
TRT-III grubunda serum VitC seviyesi yükselirken, TRT-II grubunda serum alkalen
fosfataz (ALP) ve aspartat aminotransferaz (AST) seviyeleri arttı. Bununla
birlikte VitC uygulamasının TRT-IV grubundaki tavşanlarda ALP ve AST
seviyelerini TRT-II grubuna göre azalttığı gözlendi. Elde edilen bulgulara
göre, VitC uygulamasının endosülfanın bazı zararlı etkilerine karşı erkek Yeni
Zellanda tavşanlarında koruyucu olabileceği sonucuna varıldı. 

Keywords

kan parametreleri, biyokimyasal parametreler, oksidan-antioksidan denge, endosülfan toksikasyonu, insektisit kullanımı, Vitamin C

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