Effects of Boron Administration on Hematological Parameters in Rats Given Gentamicin

Year 2018, , 140 - 147, 09.04.2018

İbrahim Durmuş , Sinan İnce , Mehmet Naci Salim , Abdullah Eryavuz , İsmail Küçükkurt

https://doi.org/10.30607/kvj.394370

Cited By: 2

Abstract

The purpse of this study is to determine the
effects of boron (B) utilisation on hematological parameters in order to
protect from gentamicin side effects.Totally 56 Wistar
Albinomale rats were randomly divided into eight groups containing 7
animal per group. While first group served as a control(fed
a diet without boron), experimental groups
were as follows; gentamicin group (100 mg/kg, i.p.), B-5 group (5 mg/kg B, i.p.),
B-10 group (10 mg/kg B, i.p.), B-20 group (20 mg/kg B, i.p.), B-5 + gentamicin
group (5 mg/kg B and 100 mg/kg gentamicin, i.p.), B-10 + gentamicin group (10
mg/kg B and 100 mg/kg gentamicin, i.p.), and B-20 + gentamicin group (20 mg/kg
B and 100 mg/kg gentamicin, i.p.). In experimental period lasted totally 14
days,B application started to be given 4 days before injection of gentamicinto
rats and was completed the fourteenth dayof the experiment. Gentamicin started
to be given to rats on the fourth day and finished on the twelfth dayof the
experiment. In the end of the experiment, the blood samples were taken from the
animals by cardiac punctureunder anaesthesia.
In the blood smaples, erythrocyte, leukocyte and platelet counts, haematocrite
value, hemoglobin concentration, mean cell volume (MCV), mean cell hemoglobin
(MCH), mean cell hemoglobine concentration (MCHC) were determined. Gentamicin
decreased leucocyte counts and haematocrite value. High boron utilization (20
mg/kg B, i.p.) decreased significantly leucocyte counts. In conclusion, the
boron supplementation has no a preventive effect on leucocyte counts reduced by
gentamicin. 

Keywords

Boron, Gentamicin, Leukocyte, Erithrocyte, Platelet, Hemoglobin

Gentamisin verilen sıçanlara bor uygulamasının hematolojik parametre düzeylerine etkileri

Abstract

Çalışma, gentamisinin
yan etkilerinden korunmak için bor (B) kullanımının hematolojik parametrelere
etkilerini belirlemek amacıyla yapıldı. Toplamda 56 adet Wistar Albino erkek sıçan her grupta 7 hayvan olacak
şekilde 8 gruba rastgele ayrıldı. İlk grup kontrol grubu olarak ayrılırken
(borsuz yemle beslendiler), deneme grupları ise, gentamisin (100 mg/kg i.p.),
B-5 (5 mg/kg B, i.p.), B-10 (10 mg/kg B, i.p.), B-20 (20 mg/kg B, i.p.), B-5 +
gentamisin (5 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-10 + gentamisin (10 mg/kg
B ve 100 mg/kg gentamisin, i.p.), B-20 + gentamisin (20 mg/kg B ve 100 mg/kg
gentamisin, i.p.) şeklinde oluşturuldu. Toplam 14 gün süren deneme döneminde,
sıçanlara gentamisin enjeksiyonundan 4 gün önce B verilmeye başlandı ve
denemenin 14.gününde tamamlandı.Gentamisin uygulamasına 4. gün başlandı ve
deneme döneminin 12. gününde bu uygulama sonlandırıldı. Denemenin sonunda,
hayvanlardan kan örnekleri anestezi altında kalpten alındı. Alınan kan
örneklerinde; eritrosit, lökosit ve trombosit sayıları ile hematokrit değer,
hemoglobin miktarı, ortalama eritrosit hacmi (MCV), ortalama eritrosit
hemoglobini (MCH) ve ortalama eritrosit hemoglobin düzeyi (MCHC) belirlendi.
Gentamisin lökosit sayısını ve hematokrit değeri düşürdü. Yüksek B uygulaması
(20 mgB/kg) lökosit sayısını önemli düzeyde azalttı. Sonuç olarak, bor
uygulamasının gentamisinin yol açtığı lökosit sayısındaki azalmayı önlemede
etkiye sahip olmadığı kanaatine varıldı.

Keywords

Bor, Gentamisin, Lökosit, Eritrosit, Trombosit, Hemoglobin

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