Bacterial and Fungal Species Isolated From Dogs With Otitis Externa

Year 2018, , 260 - 265, 01.09.2018

Belgi Diren Sığırcı , Beren Başaran Kahraman , Baran Çelik , Kemal Metiner , Serkan İkiz , A. Funda Bağcıgil , N. Yakut Özgür , Seyyal Ak

https://doi.org/10.30607/kvj.412393

Abstract

This study was
conducted to detect the distribution of bacterial and mycotic agents and the
antimicrobial susceptibility of bacterial isolates from dogs with infective
otitis externa for an 11-year period. Samples, collected from the external ear
canal of 475 dogs, were analysed by conventional bacteriological and
mycological methods between the years of 2005 and 2016. Antimicrobial
susceptibility of the isolates was determined by Kirby-Bauer disc diffusion
method. Bacterial growth was observed in 328 of 475 swab samples collected from
the dogs. Of
434 isolated bacteria, 281 isolates (64.7%) were Gram-positive cocci, 151
isolates (34.8%) were Gram-negative rods and 2 isolates (0.5%) were
Gram-positive rods. The most frequently isolated microorganisms was Staphylococcus intermedius (18.7 %),
followed by Pseudomonas aeruginosa
(12.9%), Escherichia coli (7.1%) Proteus mirabilis (6.7 %) Micrococcus spp (4.1%) and Streptococcus canis (2.5 %). Mycological
growth was also observed from 213 of 475 matching swabs. The results showed
that the need for bacterial culture and antimicrobial susceptibility tests for
appropriate antimicrobial therapy. Mycological culture should also be performed
in infectious otitis externa cases of dogs.

Keywords

Infectious otitis externa, Dog, Culture, Antimicrobial susceptibility, Microorganism

Otitis Eksternalı Köpeklerden İzole Edilen Bakteri ve Maya Türleri

Abstract

Bu çalışma, 11 yıllık bir süre boyunca infektif otit eksternaları olan
köpeklerden bakteriyel ve mikotik ajanların dağılımını ve bakteriyel
izolatların antimikrobiyal duyarlılıklarını saptamak amacıyla yapıldı.
2005-2016 yılları arasında, 475 köpeğin dış kulak kanalından toplanan
numuneler, geleneksel bakteriyolojik ve mikolojik yöntemlerle incelendi.
İzolatların antimikrobiyal duyarlılıkları Kirby-Bauer disk difüzyon yöntemi ile
belirlendi. Köpeklerden toplanan 475
sürüntü örneğinin 328'inde bakteriyolojik üreme gözlendi. İzole edilen 434
bakteriden 281’i (% 64.7) Gram pozitif
kok, 151’i (% 34.8) Gram negatif çomak ve 2 izolat (%0.5) Gram pozitif basil
olarak belirlendi. En sık izole edilen mikroorganizma Staphylococcus intermedius’tu (% 18.7),
bunu Pseudomonas aeruginosa (% 12.9),
Escherichia coli (% 7.1), Proteus mirabilis (% 6.7), Micrococcus spp (% 4.1) ve Streptococcus canis (% 2.5) izledi. Aynı
zamanda, 475 swabın 213'ünde mikolojik üreme de görüldü. Sonuçlar, uygun antimikrobiyal tedavi için bakteri kültürü ve
antimikrobiyal duyarlılık testlerine ihtiyaç duyulduğunu göstermektedir. Bunun
yanısıra, köpeklerin enfeksiyöz otitis eksterna olgularında mikolojik kültür de
yapılmalıdır.

Keywords

Kültür, Antimikrobiyal Direnç, İnfeksiyöz otitis eksterna, Köpek, Mikroorganizma

References

• 1- Bernardo FM, Martins HM, Martins ML. A survey of mycotic otitis externa of dogs in Lisbon. Rev Iberoam Micol. 1998; 15: 163-165.
• 2- Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. CLSI document M100-S16 [ISBN 1-56238-588-7]. Clinical and Laboratory Standards Institute, Pennsylvania USA. 2006
• 3- Colombini S, Merchant SR, Hosgood G. Microbial flora and antimicrobial susceptibility patterns from dogs with otitis media. Vet Dermatol. 2000; 11(4): 235-239.
• 4- Gebru E, Choi MJ, Lee SJ, Damte D, Park SC. Mutant-prevention concentration and mechanism of resistance in clinical isolates and enrofloxacin/marbofloxacin-selected mutants of Escherichia coli of canine origin. J Med Microbiol. 2011; 60(10): 1512-1522.
• 5- Guedeja‐Marrón J, Blanco JL, Ruperez C, Garcia ME. Susceptibility of bacterial isolates from chronic canine otitis externa to twenty antibiotics. Zoonoses Public Health. 1998; 45(1‐10): 507-512.
• 6- Hariharan H, Coles M, Poole D, Lund L, Page R. Update on antimicrobial susceptibilities of bacterial isolates from canine and feline otitis externa. Can Vet J. 2006; 47(3): 253-255.
• 7- Kiss G, Radvanyi SZ, Szigeti G. New combination for the therapy of canine otitis externa I Microbiology of otitis externa. J Small Anim Pract. 1997; 38(2): 51-56.
• 8- Lyskova P, Vydrzalova M, Mazurova J. Identification and antimicrobial susceptibility of bacteria and yeasts isolated from healthy dogs and dogs with otitis externa. Transbound Emerg Dis. 2007; 54(10): 559-563.
• 9- Martin Barrasa JL, Lupiola Gomez P, Gonzalez Lama Z, Tejedor Junco MT. Antibacterial susceptibility patterns of Pseudomonas strains isolated from chronic canine otitis externa. J Vet Med, Series B. 2000; 47(3): 191-196.
• 10- Morris DO, Rook KA, Shofer FS, Rankin SC. Screening of Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus schleiferi isolates obtained from small companion animals for antimicrobial resistance: a retrospective review of 749 isolates (2003–04). Vet dermatol. 2006; 17(5): 332-337.
• 11- Oliveira LC, Carvalho C, Leite CA, Brilhante RSN. Comparative study of the microbial profile from bilateral canine otitis externa. Can Vet J. 2008; 49(8): 785-788.
• 12- Penna B, Varges R, Medeiros L, Martins GM, Martins RR, Lilenbaum W. Species distribution and antimicrobial susceptibility of staphylococci isolated from canine otitis externa. Vet dermatol. 2010; 21(3): 292-296.
• 13- Quinn PJ, Markey BK, Leonard FC, FitzPatrick ES, Fanning S, Hartigan P. Veterinary microbiology and microbial disease: John Wiley & Sons. 2002.
• 14- Rosser EJ. Causes of otitis externa. Vet Clin N Am-Small. 2004; 34(2): 459-468.
• 15- Rougier S, Borell D, Pheulpin S, Woehrlé F, Boisramé B,. A comparative study of two antimicrobial/anti‐inflammatory formulations in the treatment of canine otitis externa. Vet dermatol. 2005; 16(5): 299-307.
• 16- Sarıerler M, Kırkan S. Microbiological diagnosis and therapy of canine otitis externa. Veteriner cerrahi dergisi. 2004; 10(3-4): 11-15.
• 17- Sfaciotte RAP, Bordin JT, Vignoto VKC, Munhoz PM, Pinto AA, Barbosa MJB, Cardozo RM, Osaki SC, Wosiacki SR. Antimicrobial Resistance in Bacterial Pathogens of Canine Otitis. Am J Anim Vet Sci. 2015; 10(3): 162-169.