Ratlarda Renal İskemi/Reperfüzyon Hasarında Curcumin'in Etkileri

Abstract

Bu araştırmada, Renal İskemi/Reperfüzyon Hasarı Modelinde Curcumin'in etkilerini biyokimyasal ve histopatolojik olarak araştırmak amaçlanmıştır. Çalışmada ratlar kontrol (C), sham, iskemi repürfüzyon (I/R) ve curcumin (CUR)  grubu olmak üzere (n=6) toplam 24 dişi Wistar Rat kullanılmıştır. Genel anestezi altında Grup I/R ve CUR da laparatomi uygulanarak sol böbrek pedikülü diseke edilmiş ve böbrek arteri klempe edilerek 1 saat iskemi ve 6 saat reperfüzyon gerçekleştirilmiştir. Grup CUR da 500 mg/kg Curcumin iskemi sonrası intraperitonal olarak verilmiştir. Serum MPO, IMA, MDA, NO, SOD, GPx, AOS , üre ve kreatinin ölçümleri gerçekleştirilmiştir. Doku örneklerinde MPO, MDA, NO, SOD, GPx, AOS ölçülmüştür. Histopatolojik inceleme ile böbrek dokusunda I/R hasarı skorlanmıştır. Serum ve doku AOS Grup I/R Grup CUR ile karşılaştırıldığında istatistiksel önemi olacak şekilde Grup CUR'de yüksel bulunmuştur (p<0.05). Doku NO düzeyi I/R grubu ile karşılaştırıldığında Grup CUR’da istatistiksel önemi olacak şekilde düşük bulunmuştur (15,30±5,41, 4,8±1,37) (p<0.05). Histopatolojik skorlamada Grup CUR, Grup I/R ile karşılaştırıldığında istatistiksel olarak anlamlı olacak şekilde düşük bulunmuştur (p<0.05). Sonuç olarak; bu araştırmada Curcumin’in Renal I/R hasarı modelinde serum ve doku örneklerinde oksidatif stresi azaltarak I/R hasarını önlemede olumlu etkisi olduğu ve histopatolojik inceleme ile de Curcumin'in bu olumlu etkisi desteklenmiştir.

Keywords

• Curcumin,Renal İskemi/Reperfüzyon,Rat.

Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats

Abstract

This study aimed to investigate the biochemical and histopathological effects of curcumin on Renal Ischemia/Reperfusion Injury. Twenty-four female Wistar rats were used in the study and divided into control (C), sham, ischemia/reperfusion (I/R), and curcimin (CUR) groups (n=6). Laparotomy was performed under general anesthesia in I/R and CUR groups than the left renal pedicle was dissected it was clamped. Then, 1-h ischemia and 6-h reperfusion were applied. 500 mg/kg Curcumin was given intraperitoneally to the CUR group after ischemia application. MPO, IMA, MDA, NO, SOD, GPx, AOS, urea, and creatinine levels were measured in serum samples. MPO, MDA, NO, SOD, GPx, and AOS were also measured in tissue samples. The histopathological examination was performed. Serum and tissue AOS levels were significantly higher in the CUR group than in the I/R group (P<0.05). Tissue NO levels were significantly lower in the CUR group than in the I/R group (15.30±5.41 and 4.8±1.37, respectively) (P<0.05). Histopathological scores were also significantly lower in the CUR group than in the I/R group (P<0.05). The results showed that curcumin prevented I/R damage by decreasing oxidative stress in serum and tissue samples in rat renal I/R model. 

Keywords

• Curcumin,Renal Ischemia/Reperfusion,Rats

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