Ratlarda Renal İskemi/Reperfüzyon Hasarında Curcumin'in Etkileri

Year 2018, , 215 - 222, 01.09.2018

Zülfükar Sarıtaş , Hazen Sarıtaş Musa Korkmaz , M.fatih Bozkurt , Aziz Bülbül Rengin Elsürer Afşar Tuba Berra Sarıtaş

https://doi.org/10.30607/kvj.416649

Cited By: 1

Abstract

Bu
araştırmada, Renal İskemi/Reperfüzyon Hasarı Modelinde Curcumin'in etkilerini
biyokimyasal ve histopatolojik olarak araştırmak amaçlanmıştır. Çalışmada
ratlar kontrol (C), sham, iskemi repürfüzyon (I/R) ve curcumin (CUR)  grubu olmak üzere (n=6) toplam 24 dişi Wistar
Rat kullanılmıştır. Genel anestezi altında Grup I/R ve CUR da laparatomi
uygulanarak sol böbrek pedikülü diseke edilmiş ve böbrek arteri klempe edilerek
1 saat iskemi ve 6 saat reperfüzyon gerçekleştirilmiştir. Grup CUR da 500 mg/kg
Curcumin iskemi sonrası intraperitonal olarak verilmiştir. Serum MPO, IMA, MDA,
NO, SOD, GPx, AOS , üre ve kreatinin ölçümleri gerçekleştirilmiştir. Doku
örneklerinde MPO, MDA, NO, SOD, GPx, AOS ölçülmüştür. Histopatolojik inceleme
ile böbrek dokusunda I/R hasarı skorlanmıştır. Serum ve doku AOS Grup I/R Grup
CUR ile karşılaştırıldığında istatistiksel önemi olacak şekilde Grup CUR'de
yüksel bulunmuştur (p<0.05). Doku NO düzeyi I/R grubu ile karşılaştırıldığında
Grup CUR’da istatistiksel önemi olacak şekilde düşük bulunmuştur (15,30±5,41,
4,8±1,37) (p<0.05). Histopatolojik skorlamada Grup CUR, Grup I/R ile
karşılaştırıldığında istatistiksel olarak anlamlı olacak şekilde düşük
bulunmuştur (p<0.05). Sonuç olarak; bu araştırmada Curcumin’in Renal I/R
hasarı modelinde serum ve doku örneklerinde oksidatif stresi azaltarak I/R
hasarını önlemede olumlu etkisi olduğu ve histopatolojik inceleme ile de
Curcumin'in bu olumlu etkisi desteklenmiştir.

Keywords

Curcumin, Renal İskemi/Reperfüzyon, Rat.

Effects of Curcumin on A Renal Ischemia/Reperfusion Injury in Rats

Abstract

This study aimed to
investigate the biochemical and histopathological effects of curcumin on Renal
Ischemia/Reperfusion Injury. Twenty-four female Wistar rats were used in the
study and divided into control (C), sham, ischemia/reperfusion (I/R), and curcimin
(CUR) groups (n=6). Laparotomy was
performed under general anesthesia in I/R and CUR groups than the left renal
pedicle was dissected it was clamped. Then, 1-h ischemia and 6-h reperfusion
were applied. 500 mg/kg Curcumin was given intraperitoneally to the CUR group
after ischemia application. MPO, IMA,
MDA, NO, SOD, GPx, AOS, urea, and creatinine levels were measured in serum
samples. MPO, MDA, NO, SOD, GPx, and AOS were also measured in tissue samples.
The histopathological examination was performed. Serum and tissue AOS levels
were significantly higher in the CUR group than in the I/R group (P<0.05). Tissue NO levels were
significantly lower in the CUR group than in the I/R group (15.30±5.41 and 4.8±1.37,
respectively) (P<0.05).
Histopathological scores were also significantly lower in the CUR group than in
the I/R group (P<0.05). The
results showed that curcumin prevented I/R damage by decreasing oxidative
stress in serum and tissue samples in rat renal I/R model. 

Keywords

Curcumin, Renal Ischemia/Reperfusion, Rats

References

• Adewusi E, Afolayan A. Antibacterial, antifungal and antioxidant activity of the roots and leaves of Pelargonium reniforme Curtis (Geraniaceae). African Journal of Biotechnology. 2009; 8.
• Anwaruddin S, Januzzi Jr JL, Baggish AL, Lewandrowski EL, Lewandrowski KB. Ischemia-modified albumin improves the usefulness of standard cardiac biomarkers for the diagnosis of myocardial ischemia in the emergency department setting. American journal of clinical pathology. 2005; 123, 140-145.
• Atthe BK, Babsky AM, Hopewell PN, Phillips CL, Molitoris BA, Bansal N. Early monitoring of acute tubular necrosis in the rat kidney by 23Na-MRI. American Journal of Physiology-Renal Physiology. 2009; 297, F1288-F1298.
• Dagher PC. Apoptosis in ischemic renal injury: roles of GTP depletion and p53. Kidney international. 2004; 66, 506-509.
• Döslüoglu HH, Aktan AÖ, Yeğen C, Okboy N, Yalçm A, Yahn R, Ercan S. The cytoprotective effects of verapamil and iloprost (ZK 36374) on ischemia/reperfusion injury of kidneys. Transplant international. 1993; 6, 138-142.
• Draper H, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in enzymology. 1990; 186, 421-431.
• Ellidag HY, Eren E, Aydin O, Akgol E, Yalcinkaya S, Sezer C, Yilmaz N. Ischemia modified albumin levels and oxidative stress in patients with bladder cancer. Asian Pac J Cancer Prev. 2013; 14, 2759-2763.
• Erkasap S, Erkasap N, Koken T, Kahraman A, Uzuner K, Yazihan N, Ates E. Effect of leptin on renal ischemia-reperfusion damage in rats. Journal of physiology and biochemistry. 2004; 60, 79-84.
• Grossini E, Molinari C, Pollesello P, Bellomo G, Valente G, Mary D, Vacca G, Caimmi P. Levosimendan protection against kidney ischemia/reperfusion injuries in anesthetized pigs. Journal of Pharmacology and Experimental Therapeutics. 2012; 342, 376-388.
• Guan X, Dei‐Anane G, Bruns H, Chen J, Nickkholgh A, Liang R, Gross ML, Kern M, Ludwig J, Büchler MW. Danshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation. Transplant International. 2009; 22, 232-241.
• He X, Xu X, Fan M, Chen X, Sun X, Luo G, Chen L, Mu Q, Feng Y, Mao Q. Preconditioning with hyperbaric oxygen induces tolerance against renal ischemia-reperfusion injury via increased expression of heme oxygenase-1. Journal of Surgical Research. 2011; 170, e271-e277.
• İhtiyar E, Yaşar NF, Erkasap N, Köken T, Tosun M, Öner S, Erkasap S. Effects of doxycycline on renal ischemia reperfusion injury induced by abdominal compartment syndrome. Journal of Surgical Research. 2011; 167, 113-120.
• Kettle A, Winterbourn C. Myeloperoxidase: a key regulator of neutrophil oxidant production. Redox Report. 1997; 3, 3-15.
• Korkmaz A, Kolankaya D. The protective effects of ascorbic acid against renal ischemia-reperfusion injury in male rats. Renal failure. 2009; 31, 36-43.
• Kucuk A, Kabadere S, Tosun M, Koken T, Kinaci M, Isikli B, Erkasap N. Protective effects of doxycycline in ischemia/reperfusion injury on kidney. Journal of physiology and biochemistry. 2009; 65, 183-191.
• Kumar S, Allen DA, Kieswich JE, Patel NS, Harwood S, Mazzon E, Cuzzocrea S, Raftery MJ, Thiemermann C, Yaqoob MM. Dexamethasone ameliorates renal ischemia-reperfusion injury. Journal of the American Society of Nephrology. 2009; 20, 2412-2425.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry. 1951; 193, 265-275.
• Miquel J, Bernd A, Sempere J, Dıaz-Alperi J, Ramırez A. The curcuma antioxidants: pharmacological effects and prospects for future clinical use. A review. Archives of gerontology and geriatrics. 2002; 34, 37-46.
• Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide. 2001; 5, 62-71.
• Pandya U, Saini MK, Jin GF, Awasthi S, Godley BF, Awasthi YC. Dietary curcumin prevents ocular toxicity of naphthalene in rats. Toxicology letters. 2000; 115, 195-204.
• Pompermayer K, Souza DG, Lara GG, Silveira KD, Cassali GD, Andrade AA, Bonjardim CA, Passaglio KT, Assreuy J, Cunha FQ. The ATP-sensitive potassium channel blocker glibenclamide prevents renal ischemia/reperfusion injury in rats. Kidney international. 2005; 67, 1785-1796.
• Roy D, Quiles J, Gaze D, Collinson P, Kaski J, Baxter G. Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin. Heart. 2006; 92, 113-114.
• Sancaktutar AA, Bodakci MN, Hatipoglu NK, Soylemez H, Basarılı K, Turkcu G. The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats. Urology annals. 2014; 6, 46.
• Slater TF. Free radical mechanisms in tissue injury, In: Cell Function and Disease. Springer, 1988;209-218.
• Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical chemistry. 1988; 34, 497-500.
• Tanrıverdi M, Karadağ F. Akut böbrek yetersizliği. Konuralp Tıp Dergisi. 2010; 2, 46-52.
• Thiyagarajan M, Sharma SS. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life sciences. 2004; 74, 969-985.
• Uslu H, Yoruk O, Ayyildiz A, Aktan B. Antibacterial spectrum of Umckaloabo (Pelargonium sidoides) on upper airway infection agents. Eur. J. Gen. Med. 2009; 6, 245-248.
• Yaqoob M, Edelstein CL, Wieder ED, Alkhunaizi AM, Gengaro PE, Nemenoff RA, Schrier RW. Nitric oxide kinetics during hypoxia in proximal tubules: effects of acidosis and glycine. Kidney international. 1996; 49, 1314-1319.