The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province

Year 2018, , 238 - 244, 01.09.2018

Murat Şevik

https://doi.org/10.30607/kvj.418451

Cited By: 2

Abstract

Pestiviruses are important viral agents that can cause
abortion in ruminants. In this study, roles of Border Disease Virus (BDV) and
Bovine Viral Diarrhoea Virus (BVDV) were investigated in ruminant abortion
cases. Aborted foetal tissue samples were collected from 101 animals (74 sheep
foetuses and 27 bovine foetuses), each from epidemiologically different farms,
during the months of January 2016 and December 2017 in the Afyonkarahisar
Province. One step real-time duplex RT-PCR was used for the detection of BDV
and BVDV RNA. Genetic characterization of the field isolates of pestiviruses
was conducted by sequencing 5' untranslated region (5' UTR). BDV RNA was
detected in 9 (12.16%) of the 74 aborted sheep foetuses, whereas BVDV RNA was
detected in 6 (22.2%) of the 27 bovine foetuses. Phylogenetic analysis based on
the 5' UTR region indicated that BDV isolates in the present study belong to
BDV-7 genotype whereas BVDV isolates belong to BVDV-1 genotype. The results of
this study showed that pestivirus infections play important role in ruminant
abortion cases in Afyonkarahisar province.

Keywords

Border disease virus, bovine viral diarrhoea virus, abortion, sheep, cattle

Afyonkarahisar İlinde Ruminant Abort Vakalarında Pestivirusların (BDV ve BVDV) Rolleri

Abstract

Pestiviruslar
ruminantlarda abortlara neden olan önemli viral ajanlardır. Bu çalışmada,
ruminant abort vakalarında Border Disease Virus (BDV) ve Bovine Viral Diarrhoea
Virus (BVDV)’un rolleri araştırılmıştır. Abort olmuş fötus doku örnekleri 101
hayvandan (74’ü koyun fötusu, 27’si sığır fötus), her biri epidemiyolojik olarak
farklı çiftliklerden, Ocak 2016 ve Aralık 2017 ayları arasında Afyonkarahisar
ilinden toplanmıştır. BDV ve BVDV RNA’sının tespiti için tek adımlı real-time
dubleks RT-PCR yöntemi kullanılmıştır. Sahadan izole edilen pestivirus’ların
genetik karakterizasyonu 5' translate olmayan bölge sonunun (5' UTR) sekansı
ile gerçekleştirilmiştir. BDV RNA’sı, 74 aborte koyun fötusunun 9
(%12.16)’unda, BVDV RNA’sı ise 27 sığır fötusunun 6 (%22.2)’sında tespit
edilmiştir.  5' UTR bölgesinin
filogenetik analizi bu çalışmada izole edilen BDV izolatlarının BDV-7
genotipine, BVDV izolatlarının ise BVDV-1 genotipine ait olduğunu göstermiştir.
Bu çalışmanın sonuçları, pestivirus enfeksiyonlarının, Afyonkarahisar ilindeki
ruminant abort vakalarında önemli rol oynadığını göstermektedir.

Keywords

Border disease virus, bovine viral diarrhoea virus, koyun, abort, sığır

References

• Albayrak H, Gumusova SO, Ozan E, Yazici Z. Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey. Trop Anim Health Prod. 2012; 44(4): 677-680.
• Berber E, Sozdutmaz I. Elazığ, Malatya ve Tunceli İllerinde Koyunlarda Görülen Abort Vakalarında Pestivirusların Rolünün Araştırılması. FU. Sag Bil Vet Derg. 2013; 27(1): 39-41.
• Bulut H, Sozdutmaz I, Pestil Z, Abayli H, Sait A, Cevik A. High prevalence of bovine viral diarrhea virus-1 in sheep abortion samples with pestivirus infection in Turkey. Pak Vet J. 2018; 38(1): 71-75.
• Cokcaliskan C. Gebe koyunlar ve fötuslarında Pestivirus enfeksiyonu. PhD thesis, Ankara University Health Science Institute, Ankara, 2002.
• Gur S. An investigation of border disease virus in sheep in Western Turkey. Trop Anim Health Prod. 2009a; 41(7): 1409-1412.
• Gur S, Erol N, Yapici O. Afyon, Konya ve Eskişehir İllerinde Keçilerde Pestivirus ve Parainfluenzavirus Tip 3 Enfeksiyonlarının Serolojik Olarak Araştırılması. Kocatepe Vet J. 2009b; 2(1): 23-27.
• Hasircioglu S, Kale M, Acar A. Investigation of Pestivirus Infections in Aborted Sheep and Goats in Burdur Region. Kafkas Univ Vet Fak Derg. 2009; 15(2): 163-167.
• La Rocca SA, Sandvik T. A short target real-time RT-PCR assay for detection of pestiviruses infecting cattle. J Virol Methods. 2009; 161(1): 122-127.
• Munoz-Zanzi CA, Thurmond MC, Hietala SK. Effect of bovine viral diarrhea virus infection on fertility of dairy heifers. Theriogenology, 2004; 61(6): 1085-1099.
• Nettleton PF, Gilray JA, Russo P, Dlissi E. Border disease of sheep and goats. Vet Res. 1998; 29(3-4): 327-340.
• Oguzoglu TC, Tan MT, Toplu N, Demir AB, Bilge-Dagalp S, Karaoglu T, Ozkul A, Alkan F, Burgu I, Haas L, Greiser-Wilke I. Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup? Vet Microbiol. 2009; 135(3-4): 374-379.
• Oguzoglu TC, Muz D, Yilmaz V, Alkan F, Akça Y, Burgu I. Molecular characterization of Bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey. Trop Anim Health Prod. 2010; 42(6): 1175-80.
• Ozturk D, Kale M, Pehlivanoglu F, Hasircioglu S, Turutoglu H. Evaluation for Some Bacterial and Viral Abortions of Dairy Cattle Farms in Burdur District of Turkey. Kafkas Univ Vet Fak Derg. 2012; 18(2): 255-258.
• Passler T, Walz PH. Bovine viral diarrhea virus infections in heterologous species. Anim Health Res Rev. 2010; 11(2): 191-205.
• Paton DJ, Carlsson U, Lowings JP, Sands JJ, Vilcek S, Alenius S. Identification of herd-specific bovine viral diarrhoea virus isolates from infected cattle and sheep. Vet Microbiol. 1995; 43(4): 283-294.
• Simmonds P, Becher P, Collett MS, Gould EA, Heinz FX, Meyers G, Monath T, Pletnev A, Rice CM, Stiasny K, Thiel HJ, Weiner A, Bukh J. Family Flaviviridae. In: Virus taxonomy, Eds; King AMQ, Adams MJ, Carstens EB, Lefkowitz, Ninth report of the International Committee on Taxonomy of Viruses, Elsevier, San Diego: USA. 2012; pp. 1003-1020.
• Strong R, La Rocca SA, Ibata G, Sandvik T. Antigenic and genetic characterisation of border disease viruses isolated from UK cattle. Vet Microbiol. 2010; 141(3-4): 208-215.
• Toplu N, Oguzoglu TC, Albayrak H. Dual infection of fetal and neonatal small ruminants with border disease virus and peste des petits ruminants virus (PPRV): neuronal tropism of PPRV as a novel finding. J Comp Pathol. 2012; 146(4): 289-297.
• Tuncer-Goktuna P, Alpay G, Öner EB, Yesilbag K. The role of herpesviruses (BoHV-1 and BoHV-4) and pestiviruses (BVDV and BDV) in ruminant abortion cases in western Turkey. Trop Anim Health Prod. 2016; 48(5): 1021-1027.
• Ural ZE, Erol N. Aydın ve İzmir İllerindeki Koyun ve Keçilerde Pestivirus Enfeksiyonunun Serolojik ve Virolojik Olarak Araştırılması. Harran Üniv Vet Fak Derg. 2017; 6(1): 63-68.
• Van Campen H, Frolich K. Pestivirus infections, In: Infectious Diseases of Wild Mammals, Eds; Williams ES, Barker IK, Iowa State University Press, USA. 2001; pp. 232-244.
• Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ. Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol. 1994;136(3-4): 309-323.
• Yesilbag K, Forster C, Bank-Wolf B, Yilmaz Z, Alkan F, Ozkul A, Burgu I, Rosales SC, Thiel HJ, Konig M. Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1. Vet Microbiol. 2008; 130(3-4): 258-267.
• Yesilbag K, Forster C, Ozyigit MO, Alpay G, Tuncer P, Thiel HJ, Konig M. Characterisation of bovine viral diarrhoea virus (BVDV) isolates from an outbreak with haemorrhagic enteritis and severe pneumonia. Vet Microbiol. 2014; 169(1-2): 42-49.