17β-Östradiolün Overioktomize Ratlarda Spontan Myometriyal Kasılımlar Üzerine Etkisi

Year 2018, , 374 - 384, 15.12.2018

Özlem Yıldız Gülay , Aziz Bülbül

https://doi.org/10.30607/kvj.428131

Abstract

Çalışma farklı doz ve
sürelerde uygulanan 17ß-östradiolün ovaryumları çıkarılmış sıçanlarda spontan
myometriyal kasılımlar üzerine etkisinin belirlenmesi amaçlamaktadır.
Araştırmada 3-6 aylık ve ortalama 270 ± 20 g ağırlığında, 72 adet Sprague
Dawley cinsi dişi sıçanlar kullanılmıştır. 
Sıçanlar, her grupta 18 sıçan bulunacak şekilde, kontrol (Ov) ve 3
deneme gurubuna (östrojen) ayrılmıştır. Kontrol grubuna günlük olarak kas içi
susam yağı enjeksiyonları yapılmış (0,2 ml), birinci deneme grubundaki
sıçanlara günlük 25 µg 17ß-östradiol, ikinci deneme gurubundaki sıçanlara
günlük 50 µg 17ß- östradiol, ve üçüncü deneme gurubundaki sıçanlara günlük 100
µg 17ß-östradiol kas içi uygulanmıştır. Her grup kendi arasında 3 alt gruba
ayrılarak 18, 90 ve 162. saatlerde 6’şar hayvan genel ötenazi yapılmıştır.
Nitrik oksitin etkinliğinin belirlenmesi amacıyla L-Arginin SNP ve L-NNA
(Nitro-N-Arginin) uygulaması yapıldı. Alınan uterus örneklerinden elde edilen
doku kesitlerinde cGMP-PK1 ekspirasyonunu göstermek için immunohistokimyasal
yöntemle boyandı.         Uygulama sonrası
östrojen verilen gruplarda spontan myometriyal kasılımların şiddeti, östrojenin
dozuna ve süresine bağlı olarak arttı. Ayrıca, östrojenin L-arginin-NOS-NO-cGMP
yolunun etkinliğini engellediği belirlendi. Östrojenin uterusta bulunan
longiditunal düz kaslarda cGMP-PK1 ekspirasyonu üzerine etkisinin bulunmadığı
görüldü. Sonuç olarak östrojenin doza ve süreye bağımlı olarak uterus
kasılımlarının şiddetini artırırken, L-Arginin-NOS-NO- cGMP-PK1 yolunun
etkinliğini engellediği fakat bu engellenmenin cGMP-PK1 üzerinden olmadığı
belirlenmiştir.

Keywords

17β-östradiol, Myometriyum, CGMP’ye bağımlı protein kinaz, NOS

Effects of 17β-Estradiol on Spontaneous Myometrial Contractions in Overiectomized Rats

Abstract

The aim of this study was to evaluate the effect of
injecting different doses and time intervals of 17β-estradiol on spontaneous
myometrial contractions of ovariectomized rats. Three to 6 months old 72 female
Sprague Dawley rats, weighing 270 ± 20 g, were used in the current study. The
ovariectomized rats were randomly assigned to a control (Ov) and three
experimental groups (17β-estradiol injected) of 18 rats each. Rats in the Ov
group received daily sesame oil (0.2 ml, IM), whereas each rat in the three
experimental groups was treated with daily 25, 50 and 100 μg estradiol (IM),
respectively. Each group was further divided into 3 subgroups: 6 rats in each
group were sacrificed by cervical dislocation at 18, 90 and 162 hr. In order to
determine endogenous nitric oxide activity, the L-arginine solution was
used.  Sodium nitroprusside (SNP) was
used for evaluation of the exogenous nitric oxide pathway. In addition, L-NNA
(nitro-N-arginine) treatment was applied in order to determine the effect of
endogenous NO at the receptor level. Immunohistochemical evaluation was
performed to determine the cGMP-PK1 expression from the uterus samples. In the
current study, estrogen treatment increased spontaneous myometrial contraction
in a dose and time-dependent manner. Moreover, estrogen treatments inhibited
L-arginine-NOS-NO-cGMP pathway. On the other hand, estrogen failed to have any
effect on cGMP-PK1 expression in uterine longitudinal smooth muscles.  Thus, 17β-estradiol treatments increased the
uterine smooth muscle cell contractions. The treatments also inhibited
L-Arginine-NOS-NO-cGMP-PK1 pathway. However, our results indicated that
17β-estradiol did not show its effect through cGMP-PK1.

Keywords

17β-estradiol, myometrium, cGMP-dependent protein kinase, NOS

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