Postpartum Nonsiklik Sütçü İneklerde Progesteron-Östradiol, Progesteron-GnRH-Prostaglandin F2α veya Östradiol ile Tedaviden Sonra Folliküler Değişiklikler ve Reprodüktif Performans

Abstract

Çalışmanın amacı; postpartum nonsiklik ineklerde progesteron, GnRH, PGF_2α ve östradiol benzoat hormonlarını içeren tedavi yöntemlerinin folliküler gelişim ve progesteron konsantrasyonları üzerindeki etkilerini araştırmaktır. Çalışma materyalini tedavi yöntemlerine göre rastgele 3 gruba ayrılan toplam 20 adet nonsiklik Holstein inek oluşturdu (n=20). Grup I’ de (n=7) bulunan ineklere 12 gün süre ile PRID (östradiol kapsüllü) yerleştirildi. Grup II’ de (n=6) 0. günde 10 mcg GnRH ve PRID (kapsülsüz) ve PRID’ lerin uzaklaştırıldıkları 7. günde 25 mg PGF_2α tedavileri uygulandı. Grup III’de (n=7) ise grup II’den farklı olarak kapsülsüz PRID’ lerin uzaklaştırılmalarını takiben 10. saatte östradiol benzoat uygulandı. Sıfırıncı günde GnRH uygulamalarına cevap olarak grup II (6/6) ve III’ de (5/7) bulunan ineklerde sırasıyla ortalama 2,5±0,2. ve 2,2±0,2. günlerde; 12,0±0,7 mm ve 11,0±0,9 mm çaplı folliküllerde ovulasyonlar tespit edildi. PRID’ lerin vaginadan uzaklaştırılmalarını takiben grup I’ de bulunan ineklerde (7/7) persistent follikül oluşumu gözlenirken, Grup II (4/6) ve III’ de (6/7) yer alan ineklerde 4,8±0,6. ve 3,5±0,3. günde ovulasyonlar tespit edildi. Sonuçta; Grup I’de uygulanan tedavi, siklik aktivitenin başlatılmasında başarısız kaldı ve persistent follikül oluşumuna neden oldu. Grup II ve III’ de uygulanan tedaviler siklik aktivitelerin başlatılmasında benzer başarıyı gösterirken, östradiol benzoat tedavisine bağlı olarak Grup III’ de ovulasyonlar daha erken gözlendi (P<0,05). 

Keywords

• Nonsiklik inek
• PRID
• folliküler dalga
• persistent follikül

Follicular Changes and Reproductive Performance in Postpartum Noncyclic Dairy Cows After Treatment with Progesterone and Estradiol or with Progesterone, GnRH, Prostaglandin F2α and Estradiol

Abstract

The aim of this study was to investigate the effects of treatment methods including GnRH, PGF_2α and estradiol hormones on follicular development and progesterone concentrations in postpartum noncyclic cows. The study material consisted of 20 noncyclic, Holstein breed cows (n= 20), which were randomly allocated to three groups according to the treatment protocols. In Group I (n=7), PRID with estradiol capsule was inserted for 12 d. Cows in Group II (n=6) were treated with 10 mcg of GnRH and PRID without estradiol capsule on Day 0 and 25 mg of PGF_2α on Day 7 when PRID was removed. Cows in Group III (n=7) were treated same as Group II until PRID removal, then they were received estradiol benzoate 10 h after PRID removal. In response to GnRH treatment on Day 0, all six cows in Group II and 5 of 7 cows in Group III ovulated 2,5±0,2 d and 2,2±0,2 d after GnRH treatment with 12,0±0,7 mm and 11,0±0,9 mm follicle diameter. All cows (7/7) in Group I had a persistent follicles on the ovary following 12 d PRID treatment. Ovulations after PRID removal were detected on Day 4,8±0,6 and 3,5±0,3 d in Group II(4/6) and III (6/7), respectively. In conclusion, PRID (with capsule) treatment for 12 d was not successful to resume cyclicity and cause persistent follicle in noncyclic cows. However, resumption of cyclicity was similarly high in Group II and III in response to treatmens. In addition, cows in Group III ovulated earlier than Group II due to estadiol benzoate treatment (P<0.05).

Keywords

• Noncyclic cows
• PRID
• follicular wave
• persistent follicle

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