Kısa Süreli Saklanan Koç Spermasında 7-Dehidrokolesterolün Spermatolojik Parametrelere Etkisi

Abstract

Bu çalışmanın amacı, 7-dehidrokolesterol yüklenmiş siklodekstrinin (7-DCLC) +4 C⁰’de kısa süreli saklanan koç spermasının spermatolojik parametrelerine etkisinin araştırılmasıdır. Her bir hayvandan spermalar elektroejakülatör yardımı ile sezon dışında alınarak dört eşit gruba ayrıldı, farklı oranlarda 7-DCLC (1,5 mg, 3,0 mg and 5 mg/120x10⁶)  içeren ve içermeyen kontrol (C) tris/yumurta sarısı sulandırıcı ile sulandırıldı. Sulandırılan spermalar, +4 C⁰’de saklanarak 96 saat süresince motilite, membran bütünlüğü ve anormal spermatozoa yönünden incelendi. Saklama süresi boyunca anormal spermatozoa yönünden gruplar arasında bir farklılık bulunmazken (P>0,05); motilite açısından gruplar arasında farklılıklar tespit edildi (P<0,05).  72. ve 96. zaman dilimlerinde en yüksek motilite 7-DCLC 1,5 mg/120x10⁶ grubunda tespit edildi (P<0,05). En yüksek dozlu (5 mg) 7-DCLC grubu diğer gruplarla karşılaştırıldığında spermatozoa motilitesini ve membran bütünlüğünü düşürdüğü tespit edildi. Ayrıca, 72. saatte 7-DCLC 3,0 mg grubunun membran bütünlüğü kontrol grubuna göre daha az olduğu tespit edildi (P<0,05). Sonuç olarak, 7-DCLC 1,5 mg içeren tris/yumurta sarısı sulandırıcısı koç spermasında motiliteyi ve membran bütünlüğünü +4 C⁰’de 96 saat süresince koruduğu belirlendi.

Keywords

• 7-dehidrokolesterol,siklodekstrin,motilite,membran bütünlüğü,kısa süreli saklama

Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation

Abstract

The aim of this study was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ram semen spermatological parameters on short term preservation at +4 ⁰C. Collected semen with electroejaculator at non-breeding season. Each animal native ejaculates were mixed and divided into 4 equal groups, diluated with tris/egg yolk extender with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x10⁶) and control (C). After extended semen samples were preservation at +4 ⁰C and evaluated motility, membrane integrity and abnormal spermatozoa rate until 96. hour. There were no significant differences between the groups on abnormal spermatozoa rate (P>0.05); there were a significant differences between the groups on motility at all storage period (P<0.05).The highest motility were observed in 7-DCLC 1.5 mg at 72. and 96. hour (P<0.05).  The highest dose (5 mg/120x10⁶) 7-DCLC were decreased the motility and membrane integrity compared to other groups. Besides, 7-DCLC 3.0 mg group membrane integrity was lower than control at 72. hour (P<0.05). In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membrane integrity up to 96. hour at +4°C preservation. 

Keywords

• 7-dehydrocholesterol,cyclodextrins,motility,membrane integrity,short term storage

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