Detection of Mycoplasma bovis Infection in Cattle Mammary Tissue by Immunofluorescence and qRT-PCR Methods

Abstract

Mastitis in cattle causes important economic losses in all over the world and Mycoplasma bovis is one of the important agent among pathogens shown to be responsible for this disease. The aim of this study was to determine the presence and prevalence of Mycoplasma bovis among the pathological agents implicated in mastitis by immunofluorescence staining and qRT-PCR methods in cattle mammary tissue. For this aim, 120 mammary samples with or without macroscopic lesions as this agent causes subclinical mastitis, were collected. Mastitis was diagnosed in 78 of 120 cases. Chronic mastitis cases in 56 and, acute mastitis in 22 cases were histopathologically diagnosed.  Fluorescent positivity was determined 28/120 according to the results of immunofluorescence staining for all sections. Immunpositive signs of Mycoplasma bovis were commonly observed in samples with chronic mastitis. These results were confirmed by qRT-PCR. According to these results the presence of Mycoplasma bovis agent that an important threat to herd health is 23,3%.  

Keywords

• Cattle,Subclinical Mastitis,Mycoplasma bovis,qRT-PCR,Immunoflourescence

Sığır Meme Dokularında Mycoplasma bovis Enfeksiyonunun İmmunofloresan ve qRT-PCR Yöntemleriyle Araştırılması

Abstract

Sığırlarda Mastitis tüm dünyada önemli ekonomik kayıplara neden olmaktadır.  Mycoplasma bovis ise bu hastalıktan sorumlu olarak gösterilen patojenler arasında en önemli bakteriyel etkenlerden biridir. Bu çalışmanın amacı, sığır meme dokusunda oluşan mastitisde rol oynayan patolojik ajanlar arasında Mycoplasma bovis'in varlığı ve prevalansını immunofloresan boyama ve qRT-PCR yöntemleriyle ve saptamaktır.  Bu amaçla, etkenin subklinik mastitise yol açtığı düşünülerek, makroskobik olarak lezyonlu ya da lezyonsuz olunmasına bakılmaksızın 120 adet meme örneği toplandı. Toplanan 120 örneğin 56 kronik ve 22 akut olmak üzere toplam 78 örnekte histopatolojik olarak mastitis tanısı kondu.  Tüm kesitlere uygulanan immünofloresan boyama sonuçlarına göre floresan pozitifliği 28/120 olarak belirlendi. Mycoplasma bovis immünopozitiflikleri daha yoğun olarak kronik mastitisli örneklerde gözlendi. Bu sonuçlar örnklere uygulanan qRT-PCR sonuçları ile uyumludur. Bu sonuçlara göre, Erzurum'da sürü sağlığı için önemli bir tehdit olan Mycoplasma bovis etken varlığı %23,3 oranında tespit edilmiştir. 

Keywords

• Sığır,Subklinik mastitis,Mycoplasma bovis,qRT-PCR,İmmunfloresa

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