Pathologic Examination of the Protective Effect of Glycyrrhizin on Cyclophosphamide-induced Hemorrhagic Cystitis in Rats

Abstract

Cyclophosphamide (CP) is a widely used anti-neoplastic agent in humans and small animals and also has some adverse effects in them. The most common adverse effect of CP is hemorrhagic cystitis (HS). The use of Mesna may prevent HS, but is insufficient for some side effects. In this study, the effect of glycyrrhizin (GLY), which is one of the active substances of licorice root, was examined against CP-related side effects. The experiment was designed with 49 male Sprague Dawley rats. The Control group received only physiological saline and the CP group only CP. CP+Mesna group received CP and also three dose of Mesna. The animals in the CP+GLY100 and CP+GLY200 groups were received three doses of GLY, 100 mg/kg and 200 mg/kg dose, respectively. The previous protocol was applied to the CP+Mesna+GLY100 and CP+Mesna+GLY200 groups and only the first GLY application was replaced with Mesna. In this study, it was recorded that bladder macroscopy and histopathology were better preserved in GLY applicated groups compared to CP group. However, it was observed that this protection was slightly weaker than the Mesna and GLY-Mesna combinations. From this point of view, it was observed that GLY could be preventive in CP-related HS, in addition it could also support to Mesna applications.

Keywords

• Cyclophosphamide,Glycyrrhizin,Hemorrhagic cystitis,Mesna,Pathology

Ratlarda Siklofosfamid ile İndüklenen Hemorajik Sistitisde Glycyrrhizinin Koruyucu Etkilerinin Patolojik Olarak İncelenmesi

Abstract

Siklofosfamid (CP) yaygın olarak kullanılan bir anti-neoplastik ajandır ve bazı yan etkilere sahiptir. Siklofosfamidin en belirgin yan etkisi hemorajik sistitistir (HS). Mesna kullanımı HS’yi önleyebilmekte ancak diğer bazı yan etkiler için yetersiz kalmaktadır. Bu çalışmada, CP ilişkili HS’ye karşı, meyan kökünün aktif içeriklerinden olan glycyrrhizinin (GLY) etkisi incelenmiştir. Çalışma, 49 adet, erkek Sprague Dawley rat ile dizayn edilmiştir. Kontrol grubuna sadece fizyolojik tuzlu su, CP grubuna ise sadece CP verilmiştir. CP+Mesna grubuna CP ve ayrıca 3 doz Mesna uygulanmıştır. CP+GLY100 ve CP+GLY200 gruplarına; CP ve sırasıyla 100 mg/kg ile 200 mg/kg olmak üzere üç doz GLY uygulanmıştır. Bir önceki protokol CP+Mesna+GLY100 ve CP+Mesna+GLY200 gruplarınada uygulanmış olup sadece ilk GLY uygulaması Mesna ile değiştirilmiştir. Çalışmada GLY uygulanan gruplarda CP grubuna kıyasla mesane makroskobisi ve histopatolojisinin daha iyi bir şekilde korunmuş olduğu kaydedilmiştir. Ancak, bu korumanın Mesna ve GLY-Mesna kombinasyonlarına kıyasla biraz daha zayıf kaldığı izlenmiştir. Buradan yola çıkılarak GLY’in CP ilişkili HS’de önleyici olabileceği aynı zamanda Mesna uygulamalarına destek verebileceği kanaatine varılmıştır.

Keywords

• Glycyrrhizin,Hemorajik sistitis,Mesna,Patoloji,Siklofosfamid

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