Glifosat-Bazlı Herbisite Prenatal ve Neonatal Dönemde Maruziyet Yenidoğan Rat Ovaryumunda Primordiyalden Primer Foliküle Geçişi Azaltır: Bir Ön Çalışma

Abstract

Yapılan bu çalışmada glifosat bazlı herbisit (GBH)'in yenidoğan dişi sıçanlarda embriyo döneminin 18. gününden (E18) doğum sonrası 7.gün (PND7) arasında gelişmekte olan ovaryum foliküllerinin oransal dağılımını nasıl etkilediği araştırılmıştır. Çalışmada kullanılan toplam 6 gebe sıçan; 3 gebe sıçan kontrol grubunda, 3 gebe sıçan GBH grubunda olacak şekilde 2 gruba ayrıldı. Gebe sıçanlara E18’den başlayarak E21. güne kadar günlük subkutan olarak (s.c.) deney grubuna 50 mg/kg/gün GBH ve kontrol grubuna ise taşıt madde FTS (fizyolojik tuzlu su) uygulandı. Daha sonra yenidoğan dişi yavru sıçanlara PND1’den PND7’ye kadar 2 mg/kg dozunda GBH ve taşıt madde uygulamasına devam edildi. Son ilaç uygulamadan bir gün sonra PND8’de (neonatal periyot, 6 dişi yavru/her grupta) yavru dişi sıçanlar hafif eter anestezi ile sakrifiye edildi. Diseke edilen ovaryumların histolojik incelemesi için Crossman’ın modifiye üçlü boyama yöntemi ve Periodic Acid-Shiff (PAS) boyama yöntemleri kullanılarak primordial, primer, sekonder ve preantral folikül sayıları belirlendi. GBH’ye maruz kalan dişi yavru sıçanların ovaryumları kontrol grubuna göre karşılaştırıldığında primordial foliküllerin yüzdesi önemli derecede fazla bulundu. Bununla birlikte primer, sekonder ve preantral folikül yüzdesinin ise azlığı dikkat çekti. Sonuç olarak prenatal ve neonatal GBH maruziyetinin primordial-primer folikül geçişini azalttığı gözlenmiştir.

Keywords

• Endokrin bozucular,folikül kompozisyonu,glifosat-bazlı herbisit,yenidoğan sıçan,ovaryum

Prenatal and Neonatal Exposure to Glyphosate-Based Herbicide Reduces The Primordial to Primary Follicle Transition in The Newborn Rat Ovary: A Preliminary Study

Abstract

This study investigated how a glyphosate-based herbicide (GBH) affects the proportional distribution of ovarian follicles that develop from the 18^th day of the embryo period (E18) to the 7^th postnatal day (PND7) in newborn female rats. A total of 6 pregnant rats that were used in the study were divided into two groups so that there would be 3 pregnant rats in the control group and 3 pregnant rats in the GBH group. Starting from E21 to E18 the pregnant rats in the experimental group were administered at 50 mg/kg/day GBH subcutaneously (s.c.) and the physiological saline was administered as vehicle to the control group. Subsequently, female pups received vehicle or 2 mg/kg GBH from PND1 to PND7. On PND8, all female offspring (neonatal period, 6 newborn female rats from each group) were sacrificed by light ether anesthesia. For the histological examination of the dissected ovaries, the primordial, primary, secondary and preantral follicle numbers were determined using Crossman's modified triple staining method and Periodic Acid-Shiff (PAS) staining methods. The percentage of primordial follicles was significantly higher in the ovaries of female rats in GBH exposed group compare to the control group. However, the percentage of primary, secondary and preantral follicles was lower. Thus, it was observed that prenatal and neonatal GBH exposure decreased the transition of primordial follicle to primary follicle.

Keywords

• Endocrine disruptors,follicle composition,glyphosate-based herbicide,newborn rat,ovary

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