Glifosat-Bazlı Herbisite Prenatal ve Neonatal Dönemde Maruziyet Yenidoğan Rat Ovaryumunda Primordiyalden Primer Foliküle Geçişi Azaltır: Bir Ön Çalışma

Year 2019, , 168 - 177, 30.06.2019

Ruhi Türkmen , Türkan Türkmen

https://doi.org/10.30607/kvj.543261

Abstract

Yapılan bu çalışmada
glifosat bazlı herbisit (GBH)'in yenidoğan dişi sıçanlarda embriyo döneminin
18. gününden (E18) doğum sonrası 7.gün (PND7) arasında gelişmekte olan ovaryum
foliküllerinin oransal dağılımını nasıl etkilediği araştırılmıştır. Çalışmada
kullanılan toplam 6 gebe sıçan; 3 gebe sıçan kontrol grubunda, 3 gebe sıçan GBH
grubunda olacak şekilde 2 gruba ayrıldı. Gebe sıçanlara E18’den başlayarak E21.
güne kadar günlük subkutan olarak (s.c.) deney grubuna 50 mg/kg/gün GBH ve
kontrol grubuna ise taşıt madde FTS (fizyolojik tuzlu su) uygulandı. Daha sonra
yenidoğan dişi yavru sıçanlara PND1’den PND7’ye kadar 2 mg/kg dozunda GBH ve
taşıt madde uygulamasına devam edildi. Son ilaç uygulamadan bir gün sonra
PND8’de (neonatal periyot, 6 dişi yavru/her grupta) yavru dişi sıçanlar hafif
eter anestezi ile sakrifiye edildi. Diseke edilen ovaryumların histolojik
incelemesi için Crossman’ın modifiye üçlü boyama yöntemi ve Periodic Acid-Shiff
(PAS) boyama yöntemleri kullanılarak primordial, primer, sekonder ve preantral
folikül sayıları belirlendi. GBH’ye maruz kalan dişi yavru sıçanların
ovaryumları kontrol grubuna göre karşılaştırıldığında primordial foliküllerin
yüzdesi önemli derecede fazla bulundu. Bununla birlikte primer, sekonder ve
preantral folikül yüzdesinin ise azlığı dikkat çekti. Sonuç olarak prenatal ve
neonatal GBH maruziyetinin primordial-primer folikül geçişini azalttığı
gözlenmiştir.

Keywords

Endokrin bozucular, folikül kompozisyonu, glifosat-bazlı herbisit, yenidoğan sıçan, ovaryum

Prenatal and Neonatal Exposure to Glyphosate-Based Herbicide Reduces The Primordial to Primary Follicle Transition in The Newborn Rat Ovary: A Preliminary Study

Abstract

This study investigated how a glyphosate-based
herbicide (GBH) affects the proportional distribution of ovarian follicles that
develop from the 18^th day of the embryo period (E18) to the 7^th
postnatal day (PND7) in newborn female rats. A total of 6 pregnant rats
that were used in the study were divided into two groups so that there would be
3 pregnant rats in the control group and 3 pregnant rats in the GBH group.
Starting from E21 to E18 the pregnant rats in the experimental group were
administered at 50 mg/kg/day GBH subcutaneously (s.c.) and the physiological
saline was administered as vehicle to the control group. Subsequently, female
pups received vehicle or 2 mg/kg GBH from PND1 to PND7. On PND8, all female
offspring (neonatal period, 6 newborn female rats from each group) were
sacrificed by light ether anesthesia. For the histological examination of the
dissected ovaries, the primordial, primary, secondary and preantral follicle
numbers were determined using Crossman's modified triple staining method and
Periodic Acid-Shiff (PAS) staining methods. The percentage of primordial
follicles was significantly higher in the ovaries of female rats in GBH exposed
group compare to the control group. However, the percentage of primary,
secondary and preantral follicles was lower. Thus, it was observed that
prenatal and neonatal GBH exposure decreased the transition of primordial
follicle to primary follicle.

Keywords

Endocrine disruptors, follicle composition, glyphosate-based herbicide, newborn rat, ovary

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