Protective Effect of N-Acetylcysteine on The Kidney and Liver Pathology Induced by NMDA Receptor Antagonist Mk-801 in Mice

Year 2019, , 258 - 263, 30.09.2019

Murat Sırrı Akosman , Hasan Hüseyin Demirel Ruhi Türkmen

https://doi.org/10.30607/kvj.571397

Abstract

The glutamatergic system and its receptor
N-methyl-D-aspartate (NMDA) is an important synapse mechanism in the nervous
system. The MK-801 is the blocker of this receptor and according to the
literature, the sub-chronic administration of 1mg/kg MK-801 resulted in
schizophrenia-like symptoms and degeneration in the mice brain. The aim of the
present study was to observe whether this dose of MK-801 has any pathological
effect on the liver and kidney tissues and whether N-acetylcysteine (NAC) was a
protective effect. For this purpose, the 24 Balb/C male mice were divided into
4 groups, equally. The first group was the control and 0.9% saline (10mg/kg)
was intraperitoneally (i.p.) injected. The 1mg/kg MK-801 was i.p. administered
to the second group. The third group was i.p. received 1mg/kg MK-801, 100 mg/kg
NAC and finally the 100mg/kg NAC i.p. injected to the fourth group. The drug
administration was ended on the 14^th day. The kidney and the liver
of the sacrificed mice were collected and routine histology procedure was
applied. The histopathological changes in the organs and NAC’s mostly
protective effect were noticed after the observations. In conclusion, NAC has a
partially but significantly protective effect against the MK-801 dependent
histopathological changes on the kidney and liver tissues.

Keywords

Histopathology, Kidney, Liver, Mice, MK-801, N-acetylcysteine

Supporting Institution

Afyon Kocatepe University Scientific Research Project Coordination Unit

Project Number

17.Kariyer.45

Farelerde NMDA Reseptör Antagonisti Mk-801 ile Böbrek ve Karaciğerde Oluşan Patolojik Değişikliklere Karşı N-Asetilsisteinin Koruyucu Etkisi

Abstract

Glutamaterjik sistem sinir sisteminde önemli bir sinaps mekanizmasıdır.
N-metil-D-aspartat (NMDA) reseptörleri bu sistemin önemli bir parçasıdır. NMDA
reseptörlerinin  hipofonksiyonu farelerde
kognitif ve motorik sorunlara sebep olmaktadır. MK-801 bu reseptörün oldukça
önemli bir blokörüdür. 1mg/kg MK-801’in sub-kronik uygulanması farelerde
şizofreni benzeri semptomlara ve beyinde dejenerasyona sebep olmaktadır.
Sunulan bu çalışmada MK-801’in 1mg/kg’lık sub-kronik uygulamasının farelerin
böbrek ve karaciğerlerinde patolojik etkileri olup olmadığı ve N-asetilsisteinin’in
(NAS) bu etkiler karşısında koruyucu etkisinin olup olmadığı araştırıldı. Bu
amaçla 24 adet erkek Balb/C faresi alınarak dört eşit gruba bölündü. İlk grup
kontrollerden oluştu ve bu gruba %0,9 fizyolojik tuzlu su (10mg/kg)
intraperitoneal (i.p.) yolla, ikinci gruba 1mg/kg dozunda MK-801 i.p., üçüncü
gruba 1mg/kg MK-801 ile 100mg/kg NAS birlikte i.p ve dördüncü gruba da 100mg/kg
NAS i.p. yolla verildi. İlaç uygulamaları 14 gün boyunca sürdü. Sakrifiye
edilen farelerin karaciğer ve böbrekleri alınarak bunlara rutin histoloji
prosedürü uygulandı. İncelemelerden sonra organlarda histopatolojik
değişkilikler ve NAS’ın genelde koruyucu etkisinin olduğu gözlendi. Sonuç
olarak NAS’ın böbrek ve karaciğer üzerinde MK-801’le indüklenen histopatolojik
değişikliklere karşı önemli bir koruyucu etkisinin olduğu ortaya kondu. 

Keywords

Böbrek, Fare, Histopatoloji, Karaciğer, MK-801, N-asetilsistein

Project Number

17.Kariyer.45

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