Kriptosporodiazisli Buzağılarda D-Dimer Seviyelerinin Belirlenmesi

Abstract

Bu araştırmada kriptosporodiyazisli buzağılarda kan D-dimer seviyelerinin belirlenmesi amaçlanmış olup, çalışmanın materyalini 1-3 haftalık, hızlı test kitleri ile Kriptosporidiyazis belirlenen 11 Holstein buzağı (çalışma grubu) ve 1-3 haftalık on sağlıklı Holstein buzağı (kontrol grubu) oluşturmuştur. Her buzağının, v. jugularis'lerinden alınan kan örnekleri antikoagülansız tüplere toplanmış, D-dimer konsantrasyonları, hem çalışma hem de kontrol grubu buzağılarda floresan immunoassey tekniği ile belirlenmiştir. Sonuç olarak, D-dimer seviyeleri, kontrol grubu hayvanlara kıyasla enfekte buzağılarda önemli ölçüde yüksek saptanmıştır.

Keywords

• Buzağı,Kriptosporidiazis,D-Dimer

Determination of D-Dimer Levels in Calves with Cryptosporidiosis

Abstract

The aim of the present study was emphasized to estimation of D-dimer levels among calves with cryptosporodiosis. The study was conducted on 1-3 weeks old, 11 Holstein calves with Cryptosporidiosis (study group) and on 1-3 weeks old ten healthy Holstein calves (control group). Diagnosis of Cryptosporidiosis made by rapid test kits. Blood samples were taken from each animal, by puncture of the jugular vein and were collected into plain tubes without anticoagulant. D-dimer concentrations were detected by fluorescent immunoassay techniques in both study and control group calves. As a result, D-dimer values were significantly increased in infected calves when compared with the control group animals.

Keywords

• Calf,Cryptosporidiasis,D-dimer

References

1. Kahn CM. Digestive system. The Merck Veterinary Manual. 10th ed. New Jersey; Merck & Co; 2010. p. 129-445.
2. Freyburger G, Trillaud H, Labrouche S, Gauthier P, Javorschi S, Bernard P, Grenier N. D-dimer strategy in thrombosis exclusion--a gold standard study in 100 patients suspected of deep venous thrombosis or pulmonary embolism: 8 DD methods compared. Thromb Haemost. 1998; 79(1): 32–37.
3. Goldstein NM, Kollef MH, Ward S, Gage BF. The impact of the introduction of a rapid d-dimer assay on the diagnostic evaluation of suspected pulmonary embolism. Arc Intern Med. 2001; 161(4): 567-571.
4. Nelson OL, Andreasen C. The utility of plasma d-dimer to identify thromboembolic disease in dogs. J Vet Intern Med. 2003; 17(6): 830–834.
5. Griffin A, Callan MB, Shofer FS, Giger U. Evaluation of a canine D-dimer point-of-care test kit for use in samples obtained from dogs with disseminated intravascular coagulation, thromboembolic disease, and hemorrhage. Am J Vet Res. 2003; 64(12): 1562–1569.
6. Stokol T. Plasma D-dimer for the diagnosis of thromboembolic disorders in dogs. Vet Clin N Am-Small Journal. 2003; 33(6): 1419–1435.
7. Dewhurst E, Cue S, Crawford E, Papasouliotis K. A retrospective study of canine D-dimer concentrations measured using an immuno-metric “Point-of-Care” test. J Small Anim Pract. 2008; 49 (7): 344–348.
8. Marcucci R, Prisco D, Brunelli T, Pepe G, Gori AM, Fedi S, Capanni M, Simonetti I, Federici G, Pastore A, Abbate R, Gensini GF. Tissue factor and homocysteine levels in ischemic heart disease are associated with angio graphically documented clinical recurrences after coronary angio-plasty. Thromb Haemost. 2000; 83 (6): 826–832.

9. Mammen EF. Oral contraceptive pills and hormonal replacement therapy and thromboembolic disease. Hematol Oncol Clin North Am. 2000; 14(5); 1045-1059.
10. Matyszczak L, Niedzwiedz A, Paslawska U, Nicpon J, Sikorska A. D-dimer as an indicator of intravascu-lar clotting in horses and dogs. Med Weter. 2008; 64: 272-275.
11. Pottmeyer E, Vassar MJ, Holcroft JW. Coagulation, inflammation, and responses to injury. Crit Care Clin. 1986; 2 (4): 683-703.
12. Couturaud F, Kearon C, Bates SM, Ginsberg JS. Decrease in sensitivity of D-dimer for acute venous thromboembolism after starting anticoagulant therapy. Blood Coagul Fibrinolysis. 2002; 13(3): 241-246.
13. Thomson S, Hamilton CA, Hope JC, Katzer F, Mabbott NA, Morrison LJ, Innes EA. Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies. Vet Res. 2017; 48: 42.
14. Perez-Cordon G, Yang G, Zhou B, Nie W, Li S, Shi L, Tzipori S, Feng H. Interaction of Cryptosporidium parvum with mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes. Pathog Dis. 2014;70: 17–27.
15. Guadagni F, Ferroni P, Basili S, Facciolo F, Carlini S, Crecco M, Martini F, Spila A, D'Alessandro R, Aloe S, Cerasoli V, Del Monte G, Mariotti S, Mineo TC, Roselli M. Correlation between tumor necrosis factor-alpha and D-dimer levels in non-small cell lung cancer patients. Lung Cancer. 2004; 44(3): 303-310.