Türkiye'de Subklinik Mastitisli Pırlak Koyunlardan İzole Edilen Stafilokoklarda Metisilin Direnç ve Panton-Valentine Lökosidin Genlerinin Prevalansı

Abstract

Bu çalışmada, subklinik mastitis yönünden taranan 235 Pırlak koyuna ait 464 meme lobu süt örneğinden daha önce izole edilen 47 Stafilokok türünde mecA ve pvl genlerinin varlığının araştırılması amaçlandı. Çalışmada, Pırlak koyunlardan izole edilen 13 S. aureus, 13 S. epidermidis, altı S. xylosus, beş S. chromogenes, üç S. simulans, üç S. hyicus, iki S. warneri, bir S. lentus ve bir S. saprophyticus suşu kullanıldı. Toplam 10 suşun (%21,3) mecA geni taşıdığı, bunlardan ikisinin (%4,2) ayrıca pvl genine de sahip olduğu belirlendi. mecA geni taşıyan suşlar S. aureus (3/13), S. xylosus (3/6), S. epidermidis (2/13), S. lentus (1/1) ve S. hyicus (1/3) olarak bulundu. Toplam sekiz suşta (%17,0) pvl geni belirlenirken, bunlardan altısının (%12,8) bu geni tek başına taşıdığı tespit edildi. pvl pozitif suşların üçü S. aureus, üçü S. xylosus, biri S. simulans ve biri S. hyicus olarak belirlendi. Bilgimize göre, bu çalışma Türkiye'de subklinik mastitisli Pırlak koyunlardan izole edilen Stafilokoklarda mecA ve pvl genlerinin varlığını gösteren ilk çalışmadır. 

Keywords

• Koyun,Mastitis,Metisilin Direnci,Panton-Valentine Lökosidin,Stafilokok

Prevalence of Methicillin Resistance and Panton-Valentine Leukocidin Genes in Staphylococci Isolated from Pirlak Sheep with Subclinical Mastitis in Turkey

Abstract

This study aimed to investigate the presence of mecA and pvl genes in 47 Staphylococci previously isolated from 464 half-udder milk samples belong to 235 Pirlak sheep screening for subclinical mastitis. The species from Pirlak sheep used in the present study included: 13 S. aureus, 13 S. epidermidis, six S. xylosus, five S. chromogenes, three S. simulans, three S. hyicus, two S. warneri, one S. lentus and one S. saprophyticus. A total of 10 strains (21.3%) were determined to harbour mecA gene, of these, two (4.2%) also contained pvl gene. The strains carrying mecA gene were found to be S. aureus (3/13), S. xylosus (3/6), S. epidermidis (2/13), S. lentus (1/1) and S. hyicus (1/3). The presence of pvl gene was determined in a total of eight strains (17.0%), six (12.8%) of these were alone. Of pvl positive strains, three, three, one, and one were S. aureus, S. xylosus, S. simulans and S. hyicus, respectively. To our knowledge, this is the first study showing the presence of mecA and pvl genes in the Staphylococci isolated from Pirlak sheep with subclinical mastitis in Turkey. 

Keywords

• Mastitis,Methicillin Resistance,Panton-Valentine Leukocidin,Sheep,Staphylococci

References

1. Aires-de-Sousa M. Methicillin-resistant Staphylococcus aureus among animals: current overview. Clin Microbiol Infect. 2017; 23: 373-380.
2. Aires-de-Sousa M, Parente CESR, Vieira-da-Motta O, Bonna ICF, Silva DA, de Lencastre H. Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine, and caprine milk samples collected in Rio de Janeiro State, Brazil. Appl Environ Microbiol. 2007; 73: 3845-3849.
3. Caruso M, Latorre L, Santagada G, Fraccalvieri R, Miccolupo A, Sottili R, Palazzo L, Parisi A. Methicillin-resistant Staphylococcus aureus (MRSA) in sheep and goat bulk tank milk from Southern Italy. Small Rumin Res. 2016; 135: 26-31.
4. Choi SM, Kim SH, Kim HJ, Lee DG, Choi JH, Yoo JH, Kang JH, Shin WS, Kang MW. Multiplex PCR for detection of genes encoding aminoglycoside modifying enzymes and methicillin resistance among Staphylococcus species. J Korean Med Sci. 2003; 18: 631-636.
5. Cuny C, Friedrich A, Kozytska S, Layer F, Nübel U, Ohlsen K, Strommenger B, Walther B, Wieler L, Witte W. Emergence of methicillin-resistant Staphylococcus aureus (MRSA) in different animal species. Int J Med Microbiol. 2010; 300: 109-117.
6. França CA, Peixoto RM, Cavalcante MB, Melo NF, Oliveira CJB, Veschi JA, Mota RA, Costa MM. Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil. Pesqui Vet Bras. 2012; 32: 747-753.
7. Gelasakis AI, Mavrogianni VS, Petridis IG, Vasileiou NGC, Fthenakis GC. Mastitis in sheep – The last 10 years and the future of research. Vet Microbiol. 2015; 181: 136-146.
8. Gezgen C, Seker E. Investigation of methicillin resistance and Panton-Valentine leukocidin in Staphylococci isolated from bovine mastitis. Acta Sci Vet. 2016; 44: 1373.

9. Huber H, Ziegler D, Pflüger V, Vogel G, Zweifel C, Stephan R. Prevalence and characteristics of methicillin-resistant coagulase-negative staphylococci from livestock, chicken carcasses, bulk tank milk, minced meat, and contact persons. BMC Vet Res. 2011; 7: 1-7.
10. Ikawaty R, Brouwer EC, van Duijkeren E, Mevius D, Verhoef J, Fluit AC. Virulence factors of genotyped bovine mastitis Staphylococcus aureus isolates in the Netherlands. Int J Dairy Sci. 2010; 5: 60-70.
11. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter M, Gauduchon V, Vandenesch F, Etienne J. Involvement of Panton–Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis. 1999; 295: 1128-1132.
12. Lo W, Wang C. Panton-Valentine leukocidin in the pathogenesis of community-associated methicillin-resistant Staphylococcus aureus infection. Pediatr Neonatol. 2011; 52: 59-65.
13. Martins KB, Faccioli-Martins PY, Riboli DFM, Pereira VC, Fernandes S, Oliveira AA, Dantas A, Zafalon LF, Cunha MLR. Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk. Braz J Microbiol. 2015; 46: 535-543.
14. Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Park YH, Joo YS, Koo HC. Phenotypic and genetic antibiogram of methicillin-resistant Staphylococci isolated from bovine mastitis in Korea. J Dairy Sci. 2007; 90: 1176-1185.
15. Ozenc E, Seker E, Baki Acar D, Birdane MK, Darbaz I, Dogan N. The importance of Staphylococci and threshold value of somatic cell count for diagnosis of sub-clinical mastitis in Pirlak sheep at mid lactation. Reprod Domest Anim. 2011; 46: 970-974.
16. Pantosti A. Methicillin-resistant Staphylococcus aureus associated with animals and its relevance to human health. Front Microbiol. 2012; 3: 1-12.
17. Pinho MG, Filipe SR, de Lencastre H, Tomasz A. Complementation of the essential peptidoglycan transpeptidase function of penicillin-binding protein 2 (PBP2) by the drug resistance protein PBP2A in Staphylococcus aureus. J Bacteriol. 2001; 183: 6525-6531.
18. Queiroga MC. Prevalence and aetiology of sheep mastitis in Alentejo region of Portugal. Small Rumin Res. 2017; 153: 123-130.
19. Rainard P, Riollet C. Innate immunity of the bovine mammary gland. Vet Res. 2006; 37: 369-400.
20. Sharma-Kuinkel BK, Ahn SH, Rude TH, Zhang Y, Tong SYC, Ruffin F, Genter FC, Braughton KR, DeLeo FR, Barriere SL, Fowler VG. Presence of genes encoding panton valentine leukocidin is not the primary determinant of outcome in patients with hospital-acquired pneumonia due to Staphylococcus aureus. J Clin Microbiol. 2012; 50: 848-856.
21. Strommenger B, Kettlitz C, Werner G, Witte W. Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in S. aureus. J Clin Microbiol. 2003; 41: 4089-4094.
22. Türkyılmaz S, Tekbıyık S, Oryasin E, Bozdogan B. Molecular epidemiology and antimicrobial resistance mechanisms of methicillin resistant Staphylococcus aureus isolated from bovine milk. Zoonoses Public Health. 2010; 57: 197-203.
23. Ünal N, Çinar OD. Detection of stapylococcal enterotoxin, methicillin-resistant and Panton–Valentine leukocidin genes in coagulase-negative staphylococci isolated from cows and ewes with subclinical mastitis. Trop Anim Health Prod. 2012; 44: 369-375.
24. Ünal N, Askar Ş, Macun HC, Sakarya F, Altun B, Yıldırım M. Panton–Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants. Trop Anim Health Prod. 2012; 44: 573-579.
25. Vyletělová M, Hanuš O, Karpíšková R, Šťástková Z. Occurrence and antimicrobial sensitivity in Staphylococci isolated from goat, sheep and cow's milk. Acta Univ Agric Silvic Mendel Brun. 2011; 59: 209-214.
26. Yilmaz O, Cengiz F, Ertugrul M, Wilson RT. The domestic livestock resources of Turkey: sheep breeds and cross-breeds and their conservation status. Anim Genet Resour. 2013; 52: 147-163.
27. Yoong P, Torres VJ. The effects of Staphylococcus aureus leukotoxins on the host: cell lysis and beyond. Curr Opin Microbiol. 2013; 16: 63-69.