Sivas İlinde Satılan Çiğ İnek ve Manda Sütlerinde Listeria spp. Varlığının Araştırılması

Year 2020, , 19 - 24, 31.03.2020

Seyda Şahin , Abdullah Ayyıldız

https://doi.org/10.30607/kvj.645718

Abstract

Listeria cinsi doğada yaygın
olarak bulunmakta ve halk sağlığı yönünden risk oluşturan türleri içermektedir.
Listeria türlerinin insanlara
bulaşmasından özellikle hayvansal gıdalar (et ve et ürünleri, süt ve süt
ürünleri) önem arz etmektedir. Bu nedenle, Sivas ilinde satılan çiğ inek (n=50)
ve manda sütlerinde (n=50) Listeria spp. varlığı ISO 11290-1 yöntemi
kullanılarak araştırılması çalışmanın ana amacını oluşturmaktadır. İncelenen
100 adet çiğ süt örneğinin %4’ünden (4/100) Listeria
spp. izole edildi. Süt örneklerinden elde edilen izolatların polimeraz
zincir reaksiyonu (PZR) ile doğrulanması yapıldı. Çiğ inek sütlerinin Listeria spp. kontaminasyonu %2 (1/50)
olarak belirlenirken, manda sütlerinde %6 (3/50) olarak saptandı. İnek sütünden
izole edilen izolat PZR yöntemi ile L. grayi olarak identifiye edilirken, manda
sütlerinden elde edilen izotların ise L.
seeligeri (n=2) ve L. grayi (n=1)
oldukları belirlendi. Ancak, süt örneklerinin hiçbirinde L. monocytogenes tespit edilmedi. Sonuç olarak, Sivas ilinde
satılan çiğ inek ve manda süt örneklerinin düşük bir oranının Listeria spp. ile kontamine olduğu
belirlendi. İnsanlarda hastalık oluşturan majör türün L. monocytogenes olması nedeni ile, Sivas ilinde satılan çiğ
sütlerin bu bakteri yönünden halk sağlığı sorunu yaratması ihtimalinin düşük
olduğu görülmüştür. Ancak, düzenli kontrollerin yapılması önem arz etmektedir. 

Keywords

Listeria spp., İnek sütü, Manda Sütü, PZR

Supporting Institution

Bu çalışma, Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri (CÜBAP) tarafından V-067 proje numarası ile desteklenmiştir.

Project Number

V-067

Investigation of Listeria spp. in Raw Cow and Water Buffalo Milks Sold in Sivas Province

Abstract

Listeria spp. is widely found in nature and the species pose a
risk to public health. Particularly foods obtained from animals (meat and meat
products; milk and dairy products) pose a critical risk factor for the
transmission of Listeria to humans.
Therefore, in this study, the presence of Listeria
spp. in raw cow (n=50) and water buffalo milks (n=50) that were sold in Sivas
province was investigated by using ISO 11290-1 method. Listeria spp. isolation rate was found as 4% (4/100) in overall raw
milk samples. The isolates obtained from raw milk samples were validated by
using polymerase chain reaction (PCR). The Listeria
spp. contamination rate of raw cow milk was determined as 2% (1/50) and this
rate was 6% (3/50) in water buffalo milk. It was determined that Listeria spp. isolates from the raw cow
milk was all L. grayi; for the
isolates from raw water buffalo milks, the isolates were identified as L. seeligeri (n=2) and L grayi (n=1) by PCR
method. However, none of the milk
samples was contaminated with L.
monocytogenes. Consequently, a low proportion of raw cow and buffalo milk
sold in Sivas province were contaminated with Listeria spp. Since the major Listeria
specie that is causing disease in humans is the L. monocytogenes, it was observed that the raw milks sold in Sivas
province have a slight risk for public health. However, regular monitoring is
important for that matter.  

Keywords

Listeria spp., Cow Milk, Water Buffalo Milk, PCR

Project Number

V-067

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