Kitosan Oligosakkarit (COS) Tedavisinin Oksidatif Stres Üzerine Etkileri ve Kadmiyuma Maruz Kalan Sıçanlarda Bağırsak Mikroflorası ile İlişkisi

Abstract

Bu çalışmanın amacı, kronik olarak Cd’a maruz kalan ratlarda kitosan oligosakkarit’in (COS) oksidatif stress ve bağırsak mikroflorası üzerine etkilerinin araştırılmasıdır. Hayvanlar rastgele olacak şekilde; kontrol (C; n=8), kadmiyum (Cd; n=8), kitosan oligosakkarit (COS; n=8) ve kadmiyum+kitosan oligosakkarit (Cd+COS; n=8) gruplarına ayrıldı. Daha sonra, kadmiyum klorid (CdCl2) (2mg/kg/day) Cd ve Cd+COS gruplarındaki hayvanlara haftada 3 kez 4 hafta boyunca oral yoldan verildi. Kitosan oligosakkarit de (200 mg/kg/day) COS ve Cd+COS grubundaki hayvanlara haftada 5 kez 4 hafta boyunca oral olarak uygulandı. Deneme sonunda, serum TAS, TOS seviyeleri, plasma ALT, AST, GGT, T.pro, Alb, Bil, Creat ve BUN değerleri ölçüldü. Enterobacteriaceae, Lactococcus spp. ve Lactobacillus spp. sayılarıda belirlendi. Serum TOS seviyeleri Cd grubundaki hayvanlarda COS grubundakilere oranla önemli derecede yüksek bulundu (p <0,05). Cd grubundaki hayvanların ince bağırsaklarında, kronik Cd uygulaması Lactococcus spp. sayısında 0.66 log’lık bir düşüşe sebep oldu. Sonuç olarak, her iki bileşiğin antimikrobiyel etkinliği şelat oluşumuna bağlı olarak (COS-Cd) Cd+COS grubundaki hayvanlarda azalma gösterdi.          

Keywords

• Kadmiyum,Kitosan oligosakkarit,mikroflora,oksidatif stres,rat

The Effects of Chitosan Oligosaccharide (COS) Treatment on Oxidative Stress and Its Relation with Intestinal Microflora in Rats Exposed To Cadmium

Abstract

The aim of the study was to investigate the effects of chitosan oligosaccharide (COS) treatment on oxidative stress and its relation with intestinal microflora in rats exposed to chronic cadmium toxicity. Animals were randomly divided into four groups as control (C; n=8), cadmium (Cd; n=8), chitosan oligosaccharide (COS; n=8), cadmium+chitosan oligosaccharide (Cd+COS; n = 8). After, cadmium chloride (CdCl2) (2mg /kg/ day) was orally administered to Cd and Cd+COS groups three times a week for 4 weeks. Chitosan oligosaccharide (200 mg/kg/day) was also orally administered to COS and Cd+COS groups five times a week for 4 weeks. After completion of the experiment, serum TAS, TOS levels, plasma ALT, AST, GGT, T.pro, Alb, Bil, Creat and BUN values were measured. Enterobacteriaceae, Lactococcus spp. and Lactobacillus spp. counts were also detected. Serum TOS values were detected extremely higher in Cd group animals when compared COS group (p <0,05). In the small intestine of the Cd group animals, Cd administration caused a 0.66 log decrease in the Lactococcus spp. count. In conclusion, it was found that the antimicrobial effect of both compounds decreased as a result of COS-Cd chelating in Cd + COS group.

Keywords

• Cadmium,chitosan oligosaccarides,microflora,oxidative stress,rat

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