Streptozotosin ile Diyabet Oluşturulan Ratlarda Koenzim Q10’un Bazı Kan Antioksidan Sistem Parametreleri ile Pankreas ve Aorttaki Histolojik Değişiklikler Üzerine Etkileri

Öz

Bu çalışmanın amacı diyabet oluşturulan ratlarda koenzim Q10’un antioksidan parametreleri üzerine etkilerini değerlendirmektir. Çalışmada 38 yetişkin, erkek Wistar Abino rat (250-300 gr) kullanıldı. Grup I’deki hayvanlara herhangi bir uygulama yapılmadı. Grup II’deki hayvanlara günde 0.3 ml mısır yağı dört hafta boyunca intraperitoneal olarak uygulandı. Grup III’deki hayvanlara günde 0.3 ml mısır yağında çözdürülen 10 mg/kg CoQ10 dört hafta boyunca intraperitoneal olarak uygulandı. Grup IV’deki hayvanlarda, 40 mg/kg streptozotosinin günde tek doz olmak üzere iki subkutan enjeksiyonu ile diyabet oluşturuldu. Grup V’deki hayvanlarda aynı protokolle diyabet oluşturuldu ve daha sonra bu hayvanlara dört hafta boyunca 0.3 ml mısır yağında çözdürülen 10 mg/kg CoQ10 intraperitoneal olarak uygulandı. Kan örneklerinde GSH, TBARS, SOD, NO seviyeleri ile GPx, CAT aktiviteleri belirlendi. Pankreas ve aorta doku örnekleri histolojik ve immünohistokimyasal yöntemler kullanılarak incelendi. Diyabetik grupta plazma SOD, GPx, CAT ve GSH seviyeleri control grubuna gore önemli oranda düşüktü. Diyabetik ratlara CoQ10 uygulaması ile bu parametreler diyabetik gruba göre önemli oranda arttı. Diyabetle birlikte artan plazma TBARS seviyesi, CoQ10 uygulamasıyla azaldı. Çalışmanın histolojik bulguları CoQ10 uygulaması sonucunda enzim düzeylerindeki değişiklikleri desteklemektedir. Sonuç olarak, diyabetik ratlara CoQ10 uygulaması diyabetin neden olduğu bazı olumsuz değişiklikler üzerine faydalı etkilere sahip olabilir.

Anahtar Kelimeler

• Antioksidan,Koenzim Q10,diyabet,pankreas,rat

Effects of Coenzyme Q10 on Some Blood Antioxidant System Parameters and Histological Changes in the Pancreas and Aorta of Streptozotocin-induced Diabetic Rats

Abstract

The purpose of the present study was to determine the effects of Coenzyme Q10 on antioxidant enzymes in diabetic rats. Group I was not exposed any application. Group II was intraperitoneally administrated 0.3 ml corn oil in daily for four weeks. Group III received 10 mg/kg CoQ10 in 0.3 ml corn oil intraperitoneally daily for four weeks. Diabetes was induced by subcutaneous injections of streptozotocin in group IV. Group V was made diabetic in the same way and then these animals were intraperitoneally injected with 10 mg/kg CoQ10 in 0.3 ml cornoil daily for four weeks. In blood samples, GSH, TBARS, SOD, NO levels and GPx, CAT activities were determined. Pancreas and aorta tissue samples were examined using histological and immunohistochemical methods. Plasma SOD, GPx, CAT and GSH levels in diabetic group were significantly lower than control group. These parameters significantly increased with CoQ10 application to diabetic rats when compared to diabetic group. The increased plasma TBARS level with diabetes reduced with CoQ10 treatment. The histological findings of the study support the changes in enzyme levels as a result of CoQ10 application. In conclusion, CoQ10 application to diabetic rats may have beneficial effects on some negative changes caused by diabetes.

Keywords

• Antioxidants,Coenzyme Q10,diabetes,pancreas,rats

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