Evcil Kazlarda Parazitlenen Trichostrongylus tenuis Nematodların Ekzojen Gelişim Sürelerinin Değerlendirilmesi

Öz

Bu çalışmanın amacı, evcil kazlarda parazitlenen Trichostrongylus tenuis'in eksojen gelişim ve in vitro ortamda canlı kalma sürelerini değerlendirmektir. Enfekte kuşların dışkısından izole edilen Trichostrongylus spp. yumurtaları laboratuvar ortamında 3. dönem larva elde edilmek üzere 150C, 200C ve 250C olmak üzere üç farklı sıcaklıkta kültür edilmiştir. Farklı sıcaklıklardaki larva gelişimi ve canlı kalma süreleri analiz edilmiştir. Elde edilen analiz sonuçlarına göre, T. tenuis’ in embriyonik ve postembriyonik gelişimi için optimum sıcaklık 250C ' dir. Larvalar, in vitro ortamda en hızlı gelişimi 5 günde 250C'de göstermişler ve % 83.67 hayatta kalmışlardır. Azalan sıcaklık, enfektif larvaların daha yavaş gelişimine (10 gün) ve daha kısa süre canlı kalmalarına (% 58.67) sebep olmuştur. Trichostrongylus yumurta ve larvaları her üç sıcaklık ortamında, farklı değerlerde olsa da gelişim göstermiştir. Parazitik embriyogenez, yumurtaların uzunluğunun ve genişliğinin artması ile karakterize edilmiştir. Nematodların postembriyonik gelişimi sırasında, ikinci dönem larvalarda uzunluk ve genişlikte artış gözlenirken, üçüncü dönem larvalarda uzunlukta artış, genişlikte ise azalma gözlemlenmiştir.

Anahtar Kelimeler

• Kaz,trichostrongylosis,ekzojen gelişim

Assessment of Time Periods of Exogenous Development of Trichostrongylus tenuis Nematodes, Parasitizing in Domestic Goose

Abstract

The aim of present study was to assess the time periods of exogenous development of Trichostrongylus tenuis nematodes that parasitize in domestic goose, and their survival in vitro. To do that, eggs of Trichostrongylus were obtained from excrement of infected birds and cultured in laboratory to the third stage larvae (L3). Culturing was performed at 150C, 200C and 250C. Time periods of the parasitic development was analyzed, as well as their survival. According to results, the optimum temperature of embryonic and postembryonic development of T.tenuis is 250C. The time period of exogenous development at 250C was the shortest (five days), and survival was 83.67%. Decreasing temperature resulted in slower development of infectious larvae (10 days) of lower survival (58.67%). Developmental processes in eggs and larvae of Trichostrongylus were accompanied by changes in their sizes regardless of the temperature conditions. Parasitic embryogenesis was characterized by increasing length and width of eggs. Postembryonic development of nematodes was associated with inflating length and width of second stage larvae, but inflating length and decreasing width of third stage larvae.

Keywords

• domestic goose,trichostrongyloidosis,exogenous development

References

1. Bhat SA, Khajuria JK, Katoch R, Wani MY, Dhama K. Prevalence of Endoparasites in Backyard Poultry in North Indian Region: A Performance Based Assessment Study. Asian J Anim Vet Adv. 2014; 9: 479-488.
2. Calvete C, Estrada R, Lucientes J, Estrada A, Telletxea I. Correlates of helminth community in the red-legged partridge (Alectoris rufa L.) in Spain. J Parasitol. 2003; 89(3): 445-451.
3. Cattadori IM, Haydon DT, Hudson PJ. Parasites and climate synchronize red grouse populations. Nature. 2005; 433: 737-741.
4. Cervantes-Rivera К, Villagómez-Cortés JА, Arroyo-Lara А, Landín-Grandvallet LA. A diagnostic survey of gastroenteric helminths in backyard poultry of a rural village in Mexican tropics. J Agricul Biol Sci. 2016; 11(12): 463-469.
5. Connan RM, Wise DR. Development and survival at low temperature of the free living stages of Trichostrongylus tenuis. Res Vet Sci. 1993; 55(1): 20-24.
6. Denizhan V. Karakuş A. The Prevalence of Gastrointestinal Helminths in Domestically Farmed Chicken in Van, Turkey. Kocatepe Vet J. 2019; 12(4): 443-447.
7. Dobson AP, Hudson PJ. Regulation and stability of a free-living host-parasite system: Trichostrongylus tenuis in red grouse. II. Population models. J Anim Ecol. 1992; 61(2): 487-498.
8. Elmberg J, Berg C, Lerner H, Waldenström J, Hessel R. Potential disease transmission from wild geese and swans to livestock, poultry and humans: a review of the scientific literature from a One Health perspective. Infect Ecol Epidemiol. 2017; 7(1): 1300450.

9. Enigk K, Dey-Hazra A. Propagation and treatment of the Trichostrongylus tenuis infestations. Berl Münch Tierärztl. 1971; 84(1): 11-14.
10. Fox A, Hudson PJ. Parasites reduce territorial behaviour in red grouse (Lagopus lagopus scoticus). Ecol Lett. 2001; 4: 139-143.
11. Kotelnikov GA Diagnostics of animal helminthiasis. Koloss, Мoscow. 1974; p. 240-241 (In Russian).
12. Newborn D, Foster R. Control of parasite burdens in wild red grouse Lagopus lagopus scoticus through the indirect application of anthelmintics. J Appl Ecol. 2002; 39: 909-914.
13. Romanov MN. Goose production efficiency as influenced by genotype, nutrition and production systems. World Poultry Sci J. 1999; 55 (3): 281-294.
14. Saunders LM, Tompkins DM, Hudson PJ. Spatial aggregation and temporal migration of free-living stages of the parasitic nematode Trichostrongylus tenuis. Funct Ecol. 2000; 14: 468-473.
15. Seivwright LJ, Redpath SM, Mougeot F, Watt L, Hudson PJ. Faecal egg counts provide a reliable measure of Trichostrongylus tenuis intensities in free-living red grouse Lagopus lagpus scoticus. J Helminthol. 2004; 78: 69-76.
16. Shaw JL, Moss R, Pike AW. Development and survival of the free-living stages of Trichostrongylus tenuis, a caecal parasite of red grouse Lagopus lagopus scoticus. Parasitology. 1989; 99(1): 105-113.
17. Shutler D, Alisauskas RT, Daniel McLaughlin J. Associations between body composition and helminths of lesser snow geese during winter and spring migration. Int J Parasitol. 2012; 42(8): 755-760.
18. Skírnisson К, Thorarinsdottir STh, Nielsen OK. The Parasite Fauna of Rock Ptarmigan (Lagopus muta) in Iceland: Prevalence, Intensity, and Distribution Within the Host Population. Comp Parasitol. 2012; 79(1): 44-55.
19. Wascher CAF, Bauer AC, Holtmann AR, Kotrschal К. Environmental and social factors affecting the excretion of intestinal parasite eggs in graylag geese. Behav Ecol. 2012; 23(6): 1276-1283.
20. Watson H, Lee DL, Hudson PJ. Primary and secondary infections of the domestic chicken with Trichostrongylus tenuis (Nematoda), a parasite of red grouse, with observations on the effect on the caecal mucosa. Parasitol. 1988; 97: 89-99.
21. Webster LMI, Johnson PCD, Adam А, Mable BK, Keller LF. Macrogeographic populations structure in a parasitic nematode with avian hosts. Vet Parasitol. 2007; 144: 93-103.
22. Wilson GR. The prevalence of caecal threadworms (Trichostrongylus tenuis) in red grouse (Lagopus lagopus scotісus). Oecologia. 1983; 58: 265-268.