Assessment of Morphological Features of Bull Semen Thawed at Various Temperatures and Periods of Time with CASA

Abstract

Thawing condition is one the most important factors affecting the re-animation of the spermatozoon in order to fertilise the oocyte. For that matter, we aimed to evaluate the morphological features of the head and midpiece of cryopreserved spermatozoa thawed at different temperatures and for various durations, with using CASA (Computer aided sperm analyser). Frozen semen samples belonging to the same batch, collected from three different bulls were grouped as; control group thawed for 20 seconds at 37 ℃; and experimental groups were thawed for 30, 40, 50 and 60 s at 25 ℃ and 37 ℃; for 10, 15, 20 and 25 s at 40 ℃; for 3, 6, 9, 12 s at 70 ℃. Morphometrical features of the samples were evaluated by using CASA system with nine repetitions. As a result, straws thawed at 25℃ for 40 s had the highest average length of head (6.22 ± 0.09 μm), and the width of mid-piece (0.68 ± 0.01 μm). It was concluded that the thawing temperature and duration has affected/altered the morphometry of the sperm head and midpiece, although the results were not statistically significant (p> 0.005).

Keywords

• Bull sperm
• CASA
• morphometry
• sperm head
• sperm mid-piece

Farklı Süre ve Sıcaklıklarda Çözdürülen Boğa Spermalarının Morfolojik Fonksiyonlarının CASA Cihazı ile Değerlendirmesi

Öz

Suni tohumlamada kullanılan dondurulmuş spermanın çözüm sonu parametrelerini belirleyen faktörlerin başında çözdürme koşulları gelmektedir. Yapılan araştırma ile Simental, Holstein ve Brown Swiss ırkı boğaların donmuş spermalarının, deneysel olarak belirlenen farklı sıcaklık ve sürelerde çözdürülmesi sonucu CASA cihazı ile spermatozoon başı ve orta kısmına ait parametreler bakımından değerlendirilmesi amaçlanmıştır. Çalışmada gruplar; kontrol grubu 37 ℃’de 20 saniye; deney grupları ise, 25 ℃’de 30, 40, 50 ve 60 sn.; 37 ℃’de 30, 40, 50 ve 60 sn.; 40 ℃’de 10, 15, 20 ve 25 sn.; 70 ℃’de 3, 6, 9, 12 sn. olarak belirlenmiştir. Üç farklı ırk boğaya ait, tek ejakülasyonda elde edilen dondurulmuş boğa spermaları, 9 tekrarda CASA parametreleri açısından değerlendirilmiştir. 25 ℃’de 40 sn.’de çözdürülen spermaların baş uzunluklarının (6.22 ± 0.09), orta kısım genişliğinin (0.68 ± 0.01) diğer sıcaklık ve sürelere göre daha yüksek ortalamaya sahip olduğu saptanmıştır. Çözdürme sıcaklık ve süresinin spermatozoon baş ve orta kısmına dair parametreleri değiştirdiği ancak elde edilen sonuçların istatiksel olarak anlamlı olmadığı belirlenmiştir (p> 0.005). 

Anahtar Kelimeler

• Boğa spermatozoonu
• CASA
• morfoloji
• spermatozoon başı
• spermatozoon orta kısmı

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