Protective Effect of Resveratrol and N-Acetylcysteine Combination Against Locomotor Hyperactivity Induced by MK-801

Abstract

N-Methyl-D-Aspartate (NMDA) receptors are one of the most important elements of the glutamatergic system. The hypofunction of this receptor causes locomotor hyperactivity. The chemical agent MK-801, which is an NMDA receptor antagonist, also causes locomotor hyperactivity in rodents. In the present study, it is aimed to find the lowest protective dose of neuroprotective resveratrol and N-acetyl-cysteine (NAC) combination on increased locomotor activity using MK-801 in mice. For this purpose, 84 female mice were used and 14 groups of equal number of mice were formed. Locomotor hyperactivity was created in two parts as acute (1 day drug administration) and sub-acute (4 days drug administration) phases. After drug administrations, animals were subjected to open field testing. According to the results, the drug combination was successful in reducing locomotor hyperactivity in the acute phase than in the sub-acute phase. It was observed that the intraperitoneal administration of both low doses of the combination, 40mg/kg resveratrol + 20mg/kg NAC and 20mg/kg resveratrol + 10mg/kg NAC, successfully prevented the locomotor hyperactivity in the acute phase. As a result, it was concluded that the combination of antioxidants has an effect on acutely formed locomotor hyperactivity.

Keywords

• MK-801
• N-acetylcysteine
• Antioxidant
• Locomotor Hyperactivity
• Resveratrol

Resveratrol ve N-Asetilsistein Kombinasyonunun MK-801’ le İndüklenen Lokomotor Hiperaktivite Karşısında Koruyucu Etkisi

Öz

N-Methyl-D-Aspartate reseptörleri glutamaterjik sistemin önemli bir öğesidir. Bu reseptörün hipofonksiyonu lokomotor hiperaktiviteye sebep olur. NMDA reseptörlerinin antagonisti olan MK-801 isimli kimyasal da lokomotor hiperaktiviteye sebep olmaktadır. Sunulan bu çalışmada nöroprotektif özellikleri olduğu bilinen resveratrol ve N-asetilsistein isimli iki antioksidan kombinasyonunun farelerde MK-801’le indüklenen lokomotor hiperaktivite üzerinde etkin olan en düşük dozu araştırılmıştır. Bu amaçla 84 dişi fare eşit olarak 14 gruba bölünmüştür. Lokomotor hiperaktivite akut (1 gün ilaç uygulanması) ve sub-akut (4 gün ilaç uygulanması) faz olmak üzere iki bölümde oluşturuldu. İlaç uygulamalarından sonra hayvanların katettikleri mesafe açık alan test düzeneğinde 10 dakika boyunca kaydedilmiştir. Ölçüm sonuçlarına göre antioksidan kombinasyonunun akut fazda sub-akut faza göre başarılı olduğu gözlenmiştir. Kombinasyonun her iki düşük dozu olan 40mg/kg resveratrol + 20mg/kg NAC ve 20mg/kg resveratrol + 10mg/kg NAC’ın intraperitoneal uygulamalarının akut fazda lokomotor hiperaktiviteyi başarıyla engellediği gözlendi. Sonuç olarak antioksidan kombinasyonunun akut oluşan lokomotor hiperaktivite karşısında etkisinin olduğu sonucuna varıldı.

Anahtar Kelimeler

• Antioksidan
• Lokomotor Hiperaktivite
• MK-801
• N-asetilsistein
• Resveratrol

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