Civcivlerde Dalağın Embriyonik Gelişiminin Morfohistometrik Değerlendirmesi

Abstract

Bu çalışmada, civcin dalağının belirli embriyonik dönemler göz önüne alınarak morfohistometrik gelişiminin değerlendirilmesi amaçlanmaktadır. Çalışmada, kuluçkanın 13., 16. ve 21. günlerinde 18 Babcock White Leghorn civciv embriyosundan elde edilen dalaklar kullanıldı. Rutin histolojik incelemeler ve hacim hesaplamaları için doku kesitleri Crossmon trikrom boyası ve Pappenheim’ın panoptik boyası ile boyandı ve civciv embriyolarından alınan kan örneklerinden de formül lokösitleri çıkarıldı. Ölçümler sonucunda dalak hacminde, embriyo ağırlığında ve vitellus kese ağırlığında bir artış tespit edildi. 13. ve 16.-21. günler arasında dalak hacminde fark tespit edildi. 21. gün kanındaki en yüksek lökosit oranı % 74.83 ile heterofil granülositlerde gözlenirken; en düşük lenfosit oranı % 23 olarak bulundu. Kuluçka 13. günündeki dalak kesitlerindeki damarların etrafında çok az sayıda lenfosit vardı. 16. gün kesitlerinin dalak paranşimasında sıklıkla arteryal centralis ile karşılaşıldı ve etraflarında lenfosit birikimli periarteriolar lenfoid doku oluşumunun gelişmeye başladığı görüldü. 21. günün dalak parankiminde kırmızı ve beyaz pulpa bölgelerinin kolayca ayırt edilmekteydi. Embriyonik dönemdeki dalağın parankiminde lenfosit infiltrasyonları ile karakterize edilen yapıların şekillendiği ve periferik kandaki lenfosit sayısında değişimlerin olduğu sonucuna varıldı. 

Keywords

• Civciv
• Embriyonik Gelişim
• Dalak Morfohistometrisi
• Dalak Hacmi

Morphohistometric Evaluation of Embryonic Development of Spleen in Chicken

Abstract

The aim of this study was to evaluate the morphohistometric development of chick spleen by considering specific embryonic periods. For the study, spleens obtained from 18 Babcock White Leghorn chick embryos on the 13th, 16th and 21st days of incubation were used. The sections were stained with Crossmon’s trichrome stain and Pappenheim’s panoptic stain and differential leukocyte counts were made in the blood smears. In the measurements, an increase in the spleen volume, embryo weight and vitellus sac weight were determined. There was an increase between the 13th and 16th21st days in spleen volume. The highest heterophil granulocytes (74.83%) and lowest lymphocyte ratio (23%) were found on the 21st day. On the 13th day, there were very few lymphocytes around the vessels. On the 16th day, arteria centralis were frequently encountered and periarteriolar lymphoid tissue formation with lymphocyte accumulations around them started to develop in the spleen parenchyma. The red and white pulp areas could be easily distinguished in splenic parenchyma on the 21st day. It was concluded that the structures characterised by lymphocyte infiltrations in the spleen parenchyma were formed and caused changes in the number of lymphocytes in peripheral blood during the embryonal period.

Keywords

• Chicken
• Embryonic Development
• Spleen Morphohistometry
• Spleen Volume

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