Influence of Proanthocyanidin on Motility and Osmotic Resistance Parameters of Merino Ram Sperm During Short Term Storage

Abstract

The activity of proanthocyanidin oligomers is approximately 50 times greater than that of vitamin C and vitamin E in terms of antioxidant action. The aim of this study is to investigate the effects of different doses proanthocyanidin adding to extender on ram sperm motility, membran integrity and viability test, during liquid storage of ram semen. Ejaculates were collected from four Merino rams using an electroajaculator once a week and this process was repeated six times in non-breeding season. Ejaculates were split into four aliquots and diluted to a final concentration of 150x106 spermatozoa/ml with the base extender containing proanthocyanidin (10, 50 and 100 µg/ml) and no additive (control). Diluted semen samples were transferred and stored at +4°C and sperm motility, membrane integrity and viability was analysed in determined intervals during three days storage period. Motility was evaluated subjectively by a phase contrast microscopy at 37°C. Membrane integrity and viability was analysed in hypo-osmotic resistance test with eosin staining test. After 24, 48 and 72 h, 10 μg / ml proanthocyanidin treatment sperm samples presented higher motility and HOST/E test results than control group (P <0.05) in ram semen stored at +4°C. In conclusion, it was determined that the doses of 10 μg / ml of proanthocyanidin added to extender in +4 0C storage of ram sperm showed a protective effect compared to the control group.

Keywords

• Ram semen
• Proanthocyanidin,
• Motility,
• Short term storage
• Osmotic resistance

Kısa Süreli Saklanan Merinos Koç Spermasında Proantosiyanidin Motilite ve Ozmotik Direnç Parametreleri Üzerine Etkisi

Abstract

Proantosiyanidin oligomerlerinin aktivitesi, antioksidan etki açısından C vitamini ve E vitaminine göre yaklaşık 50 kat daha fazladır. Bu çalışmanın amacı, koç spermasının kısa süreli saklanmsı sırasında, farklı dozlardaki sulandırıcıya eklenen proantosiyanidin, koç spermatozoon motilitesi, membran bütünlüğü ve canlılığı üzerine etkilerini araştırmaktır. Ejakülatlar, sezon dışında dört Merinos koçtan elektroajakülatör yardımıyla haftada bir kez toplandı ve bu işlem altı kez tekrarlandı. Ejakülatlar dört eşit parçaya bölünerek proantosiyanidin (10, 50 ve 100 ug / ml) içeren ve hiçbir katkı maddesi içermeyen (kontrol) sulandırıcılar ile nihai ml’de 150x106/ml spermatozoa olacak şekilde sulandırıldı. Sulandırılan sperma örnekleri + 4 ° C'de 72 saat süresince saklandı ve spermatozoa motilitesi, membran bütünlüğü ve canlılığı üç günlük saklama süresi boyunca değerlendirildi. Motilite, 37 ° C'de faz kontrast mikroskop ile sübjektif olarak değerlendirildi. Membran bütünlüğü ve canlılığı, eozin boyama testi ile hipoozmotik direnç testinde (HOST/E) değerlendirildi. 24, 48 ve 72 saat sonra, + 4 ° C'de saklanan koç spermasında 10 μg / ml proantosiyanidin ile muamele edilmiş spermatozoon örnekleri kontrol grubuna göre daha yüksek motilite ve HOST / Eosin test sonuçları göstermiştir (p <0,05). Sonuç olarak koç sperminin +4 oC'de saklanmasında sulandırıcıya eklenen 10 μg / ml proantosiyanidin kontrol ve diğer antioksidan gruplarına göre daha iyi bir koruyucu etki gösterdiği belirlendi.

Keywords

• Koç sperması
• Proanthocyanidin
• Motilite
• Kısa Süreli Saklama
• Ozmotik Direnç

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