Isolation of Avipoxvirus from Pigeons

Abstract

In this study, it was aimed to isolate pox virus which is the causative agent of pox disease which is commonly seen and cause serious lossess in pigeons. In the study, a total of 20 nodule samples from leisons that are clinically which are evaluated as pox nodules clinically were collected. Samples were from four different pigeon flocks each consisting of five samples. Inoculums were prepared from collected tissue samples and inoculated on the chorioallantoic membrane (CAM) of a 10 day old fertilised embryonated chicken eggs. After six to eight days after incubation, pocks were produced by the virus on CAM. Two passages were made further in order to increase the titer of the virus. DNAs were extracted from both nodules and CAM samples and isolated DNAs were amplified by PCR primers for specific for p4b gene. The presence of 578 bp amplified DNA band was regarded as positive in PCR. Nodule samples from infected pigeons were collected and for the first time pox virus from pigeons was isolated and identified molecularly in Turkey. 

Keywords

• Isolation
• Pigeon
• Pox
• PCR

Project Number

17052

Güvercinlerde Çiçek Virusu İzolasyonu

Abstract

Bu çalışmada, güvercinlerde yaygın olarak görülen ve en çok kayıp oluşturan hastalıklardan biri olan çiçek virusunun izole edilmesi amaçlandı. Çalışmada, klinik muayenede çiçek nodülü olarak değerlendirilen lezyonlar bulunan 4 farklı güvercin kümesinin her birinden 5 hayvan olmak üzere toplam 20 adet güvercinden nodül örnekleri toplandı. Toplanan doku örneklerinden inokulum hazırlanarak 10 günlük embriyolu tavuk yumurtalarında (ETY) koryoallantoik membrana (CAM) ekimler yapıldı. Yumurtalarda 6-8 gün inkubasyondan sonra CAM üzerinde poklar görüldü ve yine ETY’de 2 kez pasaj yapılarak virusun yoğunluğu artırıldı. Çiçek virusunu doğrulamak amacıyla gerek nodül örneklerinden, gerekse CAM’da görülen pok örneklerinden DNA izolasyonu yapılarak p4b geninin varlığı açısından PCR ile test edildi ve 578 bp’lik bantların görülmesi pozitif olarak değerlendirildi. Güvercinlerde görülen çiçek enfeksiyonlarında hastalardan toplanan nodül örneklerinden güvercin çiçek virusu Türkiye’de ilk kez izole edildi ve etken moleküler olarak doğrulandı.

Keywords

• Çiçek
• Güvercin
• İzolasyon
• PZR

Supporting Institution

Harran Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

17052

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