Türkiye'nin Farklı Coğrafi Bölgelerinden Toplanan Arı Poleninin Fenolik Bileşikleri ve Antioksidan Kapasitelerinin Araştırılması

Öz

Bu çalışmada, Türkiye’nin değişik bölgelerinden toplanan balarısı polenlerin toplam fenolik asit, flavonoid düzeyleri ve total antioksidan kapasiteleri araştırılmıştır. Polen numuneleri Türkiye’nin farklı bölgelerden 81 arıcıdan toplam 81 örnekten temin edilmiştir. Toplam fenolik madde içeriği kuru ağırlığın gramı başına mg gallik asit eşdeğeri (mgGAE/gr) ve toplam flavonoid analizi ise gramı başına quercetin (mgQE/gr) konsantrasyon temel alınarak spektrofotometrik bir yöntem ile yapılmıştır. Total antioksidan düzeyi indirekt metot olarak polenin DPPH (1,1-diphenyl-2-picrylhydrazyl) üzerindeki serbest radikal süpürücü etkilerine göre yapılmıştır. Çalışmamızda elde edilen bulgulara göre polenin total fenolik asit düzeyleri 7.81 ile 57.69 (mgGAE/g), toplam flavonoid düzeyleri 3.72 ile 4.97 (mgQE/g) ve DPPH ise 55.18 ile 94.08 (mg/ml) aralığında değişkenlik göstermiştir. Çalışma verilerinin Türk arı poleninin kimyasal yapısının ortaya konulmasında katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

• Antioksidan
• apiterapi
• arı poleni
• fenolik madde
• flavanoid

Investigation of Phenolic Compounds and Antioxidant Capacity of Bee Pollen Collected from Different Geographical Regions in Turkey

Abstract

The aim of this study was to evaluate the antioxidan activity, total phenolic acid, and total flavonoid content in bee pollen collected from different regions of Turkey. A total of 81 bee pollen samples from 81 beekeepers were collected from 6 different regions of Turkey. Total phenolic content of pollen samples was determined in mg gallic acid equivalent gr pollen sample (mgGAE/gr) and total flavonoid analysis was made with a spectrophotometric method in mg based on quercetin (QE/g) concentration. Total antioxidant level was conducted according to free radical scavenger effects of pollen on DPPH (1,1-diphenyl-2-picrylhydrazyl) as an indirect method. According to the results of the study, total phenolic acid levels of pollen differed between 7.81 and 57.69 (mg GAE/g), total flavonoid content differed between 3.72 and 4.97 (mg QE/g) and DPPH differed between 55.18 and 94.08 (mg/ml). It is considered that the study data will contribute to the presentation of the chemical components of Turkish bee pollen.

Keywords

• Antioxidant
• pitherapy
• bee pollen
• flavonoid
• phenolic acid

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