Ratlarda Erken Dönem Yara İyileşmesinde Etakridin Laktat ve Hipokloröz Asit Etkinliğinin Karşılaştırılması

Abstract

Bu çalışmanın amacı, etakridin laktat ve hipokloröz asidin ratlarda yara iyileşmesi üzerine etkilerinin klinik ve histopatolojik olarak karşılaştırılmasıdır. Ratlar 3 gruba ayrıldı; grup 1; kontrol grubu, grup 2; hipokloröz asid (HOCL) grubu, grup 3; etakridin laktat (EL) grubu. Her grupta 7 hayvan bulunmaktaydı. Anestezi altında, dorsal interskapular bölge derisinden 20 mm çapında tam katman deri rezeksiyonu yapıldı. Yara genişlikleri postoperatif 3., 7. ve 14. günlerde milimetrik kağıtlarla ölçüldü. Ondördüncü gün sonunda ratlar derin anestezi altında sakrifiye edildi ve yara bölgesi rezeksiyonu yapılarak histopatolojik muayene için gönderildi. Makroskopik yara muayenesinde, 14 gün sonunda HOCL grubundaki yaraların kabuk oluşmadan kapandığı, bazı yaralarda ise sadece bir skar çizgisi olduğu görüldü. Histopatolojik incelemelerde, HOCL grubu yaralarında düşük yangı hücresi, yoğun fibroblast varlığı ve düşük SOD ve GPx immunoreaktivitesi tespit edildi (P<0.05). Sonuç olarak, HOCL ile tedavi edilen hayvanlarda EL ve kontrol grubuna göre makroskopik ve histopatolojik olarak yara iyileşmesinin daha hızlı gerçekleştiği görüldü.

Keywords

• Etakridin laktat
• hipokloröz asid
• histopatoloji
• sıçan
• yara iyileşmesi

Comparision of The Efficiency of Ethacridine Lactate and Hypochlorous Acid During the Early Period of Wound Healing in Rats

Abstract

The aim of this study is to compare the effects of ethacridine lactate and hypochlorous acid on wound healing in rats through clinical and histopathological studies. The rats were divided into three groups; group 1; control group, group 2; hypochlorous acid (HOCL) group, group 3; ethacridine lactate (EL) group. Each group contained seven animals. Under anesthesia, a 20 mm long full layer skin resection was performed from dorsal interscapular region. Wound sizes were measured with millimetric paper on the 3rd, 7th and 14th day postoperatively. At the end of the 14th day, the animals were sacrificed under deep anesthesia and extensive skin resection of the wound area was performed and sent for histopathological examination. Macroscopic examination of wounds revealed that the wound was completely closed without any crust formation in the HOCL group, and also there was only a scar left in some animals of the HOCL group at the end of 14th day. Mild inflammatory cell, intense fibroblast activity and the lowest SOD and GPx immunoreactivity were found in the HOCL group compared to the other two groups (P<0.05). Consequently, it was observed that macroscopically and histopathologically, the wound healing was faster in animals treated with HOCL compared to those who were in the EL and the control group.

Keywords

• Ethacridine lactate
• histopatology
• hypochlorous acid
• rat
• wound healing

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