Effects of Two Different Immunomodulators on Calf Diarrhea in Neonatal Calves

Abstract

In the study, 18 new calves were used. The calves were divided into 3 groups in each group after the first controls. One of the groups was defined as control group (Group 1) and the other two groups as study groups (Group 2 and Group 3). Two different commercial immunomodulatory preparations were given to Group 2 and Group 3 while physiological saline was given for the placebo to the Control Group. Blood samples were collected at days 0, 3, 7, and 15 for immunoglobulin G (Ig) measurement and at days 0, 7, and 15 for hematological measurements. In hematological examination; Leukocyte (WBC), Lymphocyte (LENF), Monocyte (MON), Granulocyte (GRAN), Lymphocyte percentage (LENF%), Monocyte percentage (MON%), Granulocyte percentage (GRAN%), Red Blood Cells (RBC), Hemoglobin (HGB) Hematocrit (HCT), Mean Cell Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Cell Hemoglobin Concentration (MCHC), Red Cell Distribution Width (RDW), Thrombocyte (PLT), Mean Platelet Volume (MPV), Thrombocyte Distribution Range (PDW) was measured. Immunoglobulin G levels were measured in the sera obtained from the blood samples. As a result of the study, IgG level was determined high on the 3rd day in Group 3. In addition, IgG levels decreased in the fastest way on control group especially on the 3rd day. We think that the newborn calves have an effect on the immunoglobulin G levels in the first fifteen days after birth.

Keywords

• Diarrhea,
• calf
• İmmunomodulator

Neonatal Buzağılarda İki Farklı İmmunomodülatörün Buzağı İshalleri Üzerine Etkileri

Abstract

Sunulan çalışmada yeni doğmuş 18 adet buzağı kullanıldı. Buzağılar ilk kontrolleri yapıldıktan sonra her grupta 6 hayvan olacak şekilde 3 gruba ayrıldı. Gruplardan biri kontrol grubu (Grup 1) olarak, diğer iki grup ise çalışma grupları (Grup 2 ve Grup 3) olarak belirlendi. Kontrol grubuna plasebo amaçlı fizyolojik tuzlu su verilirken, Grup 2 ve Grup 3’e iki farklı ticari immunomodülatör preparat verildi. Vena jugularisten immünglobulin G (Ig) ölçümü için 0, 3, 7 ve 15. günler, hematolojik ölçümler için 0, 7 ve 15. günler kan örnekleri alındı. Hematolojik muayenede; Lökosit (WBC), Lenfosit (LENF), Monosit (MON), Granülosit (GRAN), Lenfosit yüzdesi (LENF%), Monosit yüzdesi (MON%), Granülosit yüzdesi (GRAN%), Alyuvar (RBC), Hemoglobin (HGB), Hematokrit (HCT), Ortalama Eritrosit Hacmi (MCV), Ortalama Hemoglobin (MCH), Bir Eritrositteki Ortalama Hemoglobin Hacmi (MCHC), Eritrosit Dağılım Genişliği (RDW), Trombosit (PLT), Ortalama Trombosit Hacmi (MPV), Trombosit Dağılım Aralığı (PDW) ölçüldü. Alınan kanlardan elde edilen serumlarda ise immunoglobulin G seviyeleri ölçüldü. Yapılan çalışma sonucunda IgG seviyesi 3. gün en yüksek Grup 3’ te tespit edildi. Ayrıca IgG seviyesi en hızlı kontrol grubunda, 3. günde düştü. Yeni doğan buzağılara 0. gün uygulanan immunomodülatörlerin yeni doğan buzağıların, doğumdan sonraki ilk on beş gündeki immunoglobulin G seviyeleri üzerine etkisi olduğunu düşünmekteyiz.

Keywords

• İmmunomodulatör
• ishal
• buzağı

References

1. Abd Ellah MR. Studying the correlations among hematological and serum biochemical constituents in cattle theileriosis. J Parasit Dis. 2013; 39(2):134-9.
2. Abel Francisco SF, Quigley JD. III. Serum immunoglobulin concentrations after feeding maternal colostrum or maternal colostrum plus colostral supplement to dairy calves. Am J Vet Res. 1993; 54(7):1051-1054.
3. Adams R, Garry FB, Aldridge BM, Holland MD, Odde KG. Hematologic values in newborn beef calves. Am J Vet Res. 1992; 53:944–950.
4. Andersen HP. Bovine Endotoxicosis Some Aspects of Relevance to Production Diseases A Review. Acta vet Scand Suppl. 2003; 98:141-155.
5. Arda M. Meme dokusunun ve sekresyonlarının immünolojik fonksiyonları; Neonatal Bağısıklık. In: Đmmunoloji, Eds, Arda, M., Minbay, A., Aydın, N., Akay, Ö., Đzgür, M., Diker, K.S., Ankara. 1994; 107-118.
6. Bangoura B, Daugschies A. Parasitological and clinical parameters of experimental Eimeria zuernii infection in calves and influence on weight gain and haemogram. Parasitol Res. 2007; 100(6):1331-40.
7. Başoğlu A, Çamkerten I, Servinç M. Serum immunoglobulin concentrations in diarrheic calves and their measurement by single radial immunodiffusion. Israel J Vet Med. 1999; 54(1):9-10.
8. Chigerwe M, Tyler JW, Summers MK, Middleton JE, Schultz LG, Nagy DW. Evaluations of factors affecting serum IgG concentration in bottle-fed calves. J Am Vet Assoc. 2009; 234(6):785-789.

9. Coles EH. Kidney Function, In Veterinary Clinical Pathology, 4th Ed, WB Saunders Company, Philadelphia. 1986; p. 171- 200.
10. Coşkun, A., Şen, I. Acute phase response and clinical changes in calves with lipopolysaccharide induced endotoxemia. Eurasian Journal of Veterinary Sciences, 2012; 28:21-26.
11. Egli CP, Blum JW. Clinical, haematological, metabolic and endocrine traits during the first three months of life of suckling simmentaler calves held in a cow-calf operation. Transbound Emerg Dis, 1998; 45:99–118.
12. Erdoğan HM, Ünver A, Citil M, Güneş V, Arslan MÖ, Tuzcu M, Gökce HG. Dairy farming in Kars district, Turkey: III. Neonatal calf health. Turk J Vet Anim Sci. 2009; 33(3):185-192.
13. Friedrichs KR, Harr KE, Freeman KP, Szladovits B, Walton RM, Barnhart KF, Blanco-Chavez J. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics. Vet Clin Pathol. 2012; 41:441–453.
14. İstanbulluoğlu E. Septicaemia neonatorumlu buzağılardan izole edilen Escherichia coli suşlarının biyokimyasal, serolojik, enterotoksijenik, antibiyotiklere duyarlılık, bulaşıcı tip plasmd (R-faktör) taşıma özellikleri ile infekte ve normal buzağılardan elde edilen serum örneklerinin immünoglobulin miktarları (lgG, IgA) üzerinde incelemeler. Doçentlik Tezi, Ankara,1978.
15. Jain NC. Schalm's Veterinary Hematology. 4th edn. Philadelphia,1986. Jones ML, Alison RW. Evaluation of the ruminant complete blood cell count. Vet Clin North Am Food Anim Pract. 2007; 23:377–402.
16. Kalınbacak A. İshalli Buzağıların Sıvı Sağaltımında Hipertonik Salin-Dextran ve Oral Elektrolit Solüsyonunun Kullanımı. Ankara Üniv Vet Fak Derg. 2003; 50:113- 118.
17. Knowles TG, Edwards JE, Bazeley KJ. Changes in the blood biochemical and haematological profile of neonatal calves with age, Veterinary Record. 2000; 147:593-598.
18. Koçkaya M, Özşensoy Y. Determınatıon Of Some Blood Parameters And Macro Elements In Coccıdıosıs Affected Akkaraman Kangal Lambs. Journal of Asian Scientific Research. 2016; 6(9):138-142.
19. Matte JJ, Girard CL, Seoane JR. Absorption of colostral immunoglobulin G in the newborn dairy calf. J Dairy Sci. 1982; 65:1765-1772.
20. Mohri M, Sharifi K, Eidi S. Hematology and serum biochemistry of Holstein dairy calves: age related changes and comparison with blood composition in adults. Res Vet Sci. 2007; 83:30–39.
21. Osame S, Ichijo S, Ohta C, Watanabe T, Benkele W, Goto H. Efficacy of colostral immunoglobulins for therapeutic and preventive treatments of calf diarrhea. J Vet Med Sci. 1991; 53(1):87-91.
22. Öcal N, Duru SY, Yağcı BB, Gazyağcı S. İshalli buzağılarsa asit-baz dengesi bozukluklarının saha şartlarında tanı ve sağaltımı. Kafkas Üniv Vet Fak Derg. 2006; 12(2):175-183.
23. Panousis N, Siachos N, Kitkas G, Kalaitzakis E, Kritsepi-Konstantinou M, Valergakis GE. Hematology reference intervals for neonatal Holstein calves. Res Vet Sci. 2018; 118:1-10.
24. Radostits OM, Blood DC, Gay CC.Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. London, Bailliere Tindall, 1994.
25. Raleigh RJ, Wallace JD. The influence of iron and copper on hematologic values and on body weight of range calves. Am J Vet Res. 1962; 23:296–299.
26. Ramin AG, Asri-Rezaei S, Paya K, Eftekhari Z, Jelodary M, Akbari H, Ramin S. Evaluation of Anemia in Calves up to 4 Months of Age in Holstein Dairy Herds. İstanbul Univ Vet Fak Derg. 2014; 40(1):1-6.
27. Rick M. Colostrum. The Key to Calf Survival, www.merricks.com, 2005.
28. Selim SA, Smith BP, Cullar JS. Serum Immunoglobulins in Calves: Their Effects and Two Easy Reliable Means of Measurement. Vet Med. 1995; 4:387- 404.
29. Şahal M, Ünsüren H, Kurtdede A, İmren HY, Kalınbacak A, Börkü MK, Özlem MB. Yeni Doğan İshalli Buzağıların Klinik Bulguları ve Asit-Baz Dengesi Dikkate Alınarak Sodyum Bikarbonat ve Elektrolitik Sıvılarla Sağaltımı. Ankara Üniv Vet Fak Derg. 1994; 41(3-4):509- 525.
30. Tennant B, Harrold D, Reina-Guerra M, Kendrick JW, Laben RC. Hematology of the neonatal calf: erythrocyte and leukocyt values of normal calves. Cornell Vet. 1974; 64:516–532.
31. Threatte GA. Usefulness of the mean platelet volume. Clin Lab Med. 1993; 13:937.
32. Turgut K. Veteriner klinik laboratuvar teşhis. Ankara: Genişletilmiş 2. Baskı, Bahçıvanlar Basım Sanayi AŞ, 2000.
33. Tyler JW, Parish SM, Besser TE. Detection of low serum immunoglobulin concentrations in clinically ill calves. J Vet Intern Med. 1999; 13(1):40-43.
34. Waldner CL, Rosengren LB. Factors associated with serum immunoglobulin levels in beef calves from Alberta and Saskatchewan and association between passive transfer and health outcomes. Can Vet J. 2009; 50:275–281.
35. Weaver DM, Tyler FW, Vanmetre DC, Hostetler DE, Barrington GM. Passive Transfer of Colostral Immunoglobulins in Calves. J Vet Intern Med. 2000; 14:569-577.
36. White DG. Colostral supplementation in ruminants. The Compendium on continuing education for the practicing veterinarian. 1993; 15:335-342.
37. Zanker IA, Hammon HM, Blum JW. Dealyed feeding of first colostrums: are there prolonged effects on haematological, metabolic and endocrine parameters and on growth performance in calves? J Anim Physiol and Anim Nut. 2001; 53-66.