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Ankara Keçisinde Glandula Mandibularis Mast Hücreleri Üzerine Histokimyasal ve İmmünohistokimyasal Bir Çalışma

Year 2021, , 255 - 261, 30.06.2021
https://doi.org/10.30607/kvj.899909

Abstract

Mast hücreleri genellikle dış çevre ile arayüz oluşturan yüzeylere yakın yapılarla, özellikle deri, solunum ve sindirim sistemleri ile ilişkilidir. Bu çalışma, Ankara keçisi glandula mandibularisinde mast hücrelerinin dağılımını ve heterojenliğini morfolojik, histokimyasal ve immünohistokimyasal yöntemlerle göstermek amacıyla yapılmıştır. Toplam yedi adet yetişkin sağlıklı erkek Ankara keçisinin glandula mandibularisi incelenmiştir. Toluidin mavisi ile boyanan kesitlerde, mast hücreleri, metakromatik boyanmaları ile belirgin bir şekilde ayırt edildi. Hücreler, özellikle yuvarlak, oval ve iğ şeklinde olmak üzere çeşitli boyut ve şekillerde gözlendi. Mast hücreleri glandula mandibularis'de hem intralobuler hem de interlobular interstisyumda görüldü. İnterlobüler interstisyumda, özellikle kan damarlarının çevresinde birçok mast hücresi gözlendi. Mast hücre heterojenliğini belirlemek için Alcian-blue / Safranin O kombine boyama yöntemi kullanıldı. Glandula mandibularis'te mavi renkli alcian-blue (AB) (+) ve pembe-kırmızı renkli safranin O (SO) (+) mast hücre alt tipleri görüldü. Kimaz pozitif mast hücreleri genellikle tek tek hem intralobüler hem de interlobüler intersitisyumda gözlendi. Sonuç olarak, yerel bir tür olan Ankara keçisinin glandula mandibularisi incelenerek; mast hücrelerinin morfolojisi, konumu, heterojenitesi ve kimaz ekspresyonu belirlendi.

References

  • Atiakshin D, Buchwalow I, Tiemann M. Mast cell chymase: morphofunctional characteristics. Histochem Cell Biol. 2019; 152: 253-269. doi:10.1007/s00418-019-01803-6.
  • Yadav A, Desai RS, Bhuta BA, Singh JS, Mehta R, Nehete AP. Altered ımmunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium. Plos One. 2014; 9(5): e98719. doi:10.1371/journal.pone.0098719.

A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat

Year 2021, , 255 - 261, 30.06.2021
https://doi.org/10.30607/kvj.899909

Abstract

Mast cells are particularly in association with structures, especially the skin, respiratory, and digestive systems in proximity to surfaces that interface with the external environment. This study was carry out to demonstrate the distribution and heterogeneity of mast cells in the Angora goat glandula mandibularis by using morphological, histochemical, and immunohistochemical methods. A total of seven healthy male adult Angora goats' mandibular glands were studied. Mast cells were distinctly distinguished by their metachromatic staining in preparates stained with toluidine blue. The cells were observed in various sizes and shapes, especially round, oval, and elongate-shaped. Mast cells were seen in both intralobular and interlobular interstitium in glandula mandibularis. Many mast cells were observed in the interlobular interstitium, especially around the blood vessels. The Alcian-blue/Safranin O combined staining method was used to determine mast cell heterogeneity. In glandula mandibularis, blue-colored alcian-blue (AB) (+) and pink-red colored safranin O (SO) (+) mast cell subtypes were observed. Chymase positive mast cells were usually observed one by one in both intralobular and interlobular interstitium. As a result, the mandibular gland of Angora goat which is local species was examined; the morphology, locations, heterogeneity of mast cells, and chymase expression were specified.

References

  • Atiakshin D, Buchwalow I, Tiemann M. Mast cell chymase: morphofunctional characteristics. Histochem Cell Biol. 2019; 152: 253-269. doi:10.1007/s00418-019-01803-6.
  • Yadav A, Desai RS, Bhuta BA, Singh JS, Mehta R, Nehete AP. Altered ımmunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium. Plos One. 2014; 9(5): e98719. doi:10.1371/journal.pone.0098719.
There are 2 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section RESEARCH ARTICLE
Authors

Tuğrul Ertuğrul 0000-0002-9310-1200

Publication Date June 30, 2021
Acceptance Date May 24, 2021
Published in Issue Year 2021

Cite

APA Ertuğrul, T. (2021). A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat. Kocatepe Veterinary Journal, 14(2), 255-261. https://doi.org/10.30607/kvj.899909
AMA Ertuğrul T. A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat. kvj. June 2021;14(2):255-261. doi:10.30607/kvj.899909
Chicago Ertuğrul, Tuğrul. “A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat”. Kocatepe Veterinary Journal 14, no. 2 (June 2021): 255-61. https://doi.org/10.30607/kvj.899909.
EndNote Ertuğrul T (June 1, 2021) A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat. Kocatepe Veterinary Journal 14 2 255–261.
IEEE T. Ertuğrul, “A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat”, kvj, vol. 14, no. 2, pp. 255–261, 2021, doi: 10.30607/kvj.899909.
ISNAD Ertuğrul, Tuğrul. “A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat”. Kocatepe Veterinary Journal 14/2 (June 2021), 255-261. https://doi.org/10.30607/kvj.899909.
JAMA Ertuğrul T. A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat. kvj. 2021;14:255–261.
MLA Ertuğrul, Tuğrul. “A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat”. Kocatepe Veterinary Journal, vol. 14, no. 2, 2021, pp. 255-61, doi:10.30607/kvj.899909.
Vancouver Ertuğrul T. A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat. kvj. 2021;14(2):255-61.

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