Comparison of Fat and Carbohydrate Metabolisms in Chicken and Rat Liver

Abstract

The aim of this study is to reveal histological differences between prenatal and adult liver carbohydrate and fat metabolism in chickens and rats. In this study, 30 Wistar albino rats and 30 Ross breed broiler chickens were used. The rats and chickens were divided into groups of 10. These groups were categorized as adult, 14- and 18-day-old fetus groups. In this study, the fat and carbohydrate metabolisms in rat and chicken liver were studied by histological methods, and changes in adult and fetal periods were evaluated comparatively. In the light microscopic examinations of liver carbohydrate accumulation, hepatic glycogen accumulation was similar in the adult rats and chickens. When the hepatic glycogen amount was compared in the 14-day-old rat and chicken fetuses, while glycogen granules were found in most of the chicken fetal hepatocytes, almost no glycogen granules were found in the rat fetuses. This difference between the animals was found to be statistically significant (p<0.001). It was observed that glycogen accumulation in the 18-day-old rat fetuses was less than that in chicken fetuses. This difference between the animals was found to be statistically significant (p=0.013). Light microscopic examinations of liver lipid accumulation showed that similar ratios of lipid droplets were observed in the hepatocytes of adult rats and chickens. Significantly higher amounts of lipid droplets were detected in the 14- and 18-day-old chicken fetus hepatocytes compared with 14- and 18-day-old rat fetus hepatocytes, which was statistically significant (p<0.001).

Keywords

• chickens
• fetüs
• hepatocyte
• rats

Project Number

18L0239018

Tavuk ve Rat Karaciğerinde Yağ ve Karbonhidrat Metabolizmalarının Karşılaştırılması

Abstract

Bu çalışmanın amacı, tavuklarda ve ratlarda doğum öncesi ve erişkin dönem karaciğer karbonhidrat ve yağ metabolizması arasındaki histolojik farklılıkları ortaya koymaktır. Çalışmada, Wistar albino türü 30 adet rat ile 30 adet Ross ırkı broiler tavuk kullanıldı. Ratların ve tavukların her biri 10’ arlı gruplara ayrıldı. Bu gruplar erişkin, 14 günlük ve 18 günlük fötuslar halinde düzenlendiler. Bu çalışmada, rat ve tavuk karaciğerindeki yağ ve karbonhidrat metabolizmaları histolojik yöntemlerle incelenmiş, erişkin ve fetal dönemlerdeki değişimler karşılaştırmalı olarak değerlendirilmiştir. Karaciğer karbonhidrat birikiminin ışık mikroskobik incelemelerinde, Erişkin dönem ratlar ile tavuklarda hepatik glikojen birikiminin benzer yoğunlukta olduğu görüldü. 14 günlük rat ve tavuk fötuslarında hepatik glikojen miktarı karşılaştırıldığında, tavuk fötus hepatositlerinin çoğunda glikojen granüllerine rastlanırken rat fötuslarında yok denecek kadar az miktarda glikojen granülüne rastlandı. Hayvanlar arasındaki bu fark istatistiksel olarak da anlamlı bulundu (p<0.001). 18 günlük rat fötuslarındaki glikojen birikiminin tavuk fötuslarına göre daha az miktarda olduğu gözlendi. Hayvanlar arasındaki bu fark istatistiksel olarak da anlamlı bulundu (p=0.013). Karaciğer lipid birikiminin ışık mikroskobik incelemelerinde, erişkin rat ve tavuk hepatositlerinde benzer oranlarda lipid damlacığı görüldü. 14 ve 18 günlük tavuk fötusu hepatositlerinde 14 ve 18 günlük rat fötuslarına oranla belirgin derecede fazla miktarda lipid damlacığı tespit edildi. Hayvanlar arasındaki bu farklar istatistiksel olarak da anlamlı bulundu (p<0.001).

Keywords

• fetus
• hepatosit
• rat
• tavuk

Supporting Institution

Ankara Üniversitesi Bilimsel Araştırma Projeleri Müdürlüğü (AUBAP)

Project Number

18L0239018

Thanks

Ankara Üniversitesi Bilimsel Araştırma Projeleri Müdürlüğü (AUBAP)

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