Hepatoprotective Effect of Pomegranate (Punica Granatum L.) in A Rabbit Model of Steatohepatitis

Abstract

The study aimed to exhibit liver protection, activity, and probable side effects of LPJE on a steatohepatitis rabbit model. In this study, 48 rabbits were used. The first group (Group 1) rabbits were fed a standard diet (SD), while the second group (Group 2) rabbits were fed a high-fat diet (HFD). In two groups, three different doses of LPJE (25 mg/kg, 50 mg/kg, and 100 mg/kg) were given simultaneously to the rabbits for eight weeks. Results showed that the serum albumin concentration was significantly different in the LPJE group (100 mg/kg) than in the positive control group. The cholesterol concentration of the LPJE (50mg/kg) administered subgroup of Group 2 (96.71 ± 12.03) was found to be the lowest. According to histopathological examination results, Group 2 did not display a statistical difference; however, the lowest liver fat deposition degree was 26.49 ± 8.64% in the LPJE (50 mg/kg) subgroup. As a result of this study, the lyophilized pomegranate extract has hepatoprotective activity. The most effective dose was 50 mg/kg on the non-alcoholic steatohepatitis (NASH) rabbit model. No side effects were determined up to 100 mg/kg pomegranate juice extract on rabbits.

Keywords

• : Punica granatum
• antioxidant activity
• bioactivity
• non-alcoholic steatohepatitis

Bir Tavşan Steatohepatit Modelinde Narın (Punica Granatum L.) Hepatoprotektif Etkisi

Abstract

Bu çalışmada, bir steatohepatit tavşan modelinde Liyofilize nar suyu ekstresinin (LPJE) karaciğer koruması, aktivitesi ve olası yan etkilerinin gösterilmesi amaçlandı ve 48 tavşan kullanıldı. Birinci grup (Grup 1) tavşanlara standart bir diyet (SD), ikinci grup (Grup 2) tavşanlara ise yüksek yağlı bir diyet (HFD) verildi. Her iki gruptaki üçer alt grup tavşanlara sekiz hafta boyunca eş zamanlı olarak üç farklı dozda LPJE (25 mg/kg, 50 mg/kg ve 100 mg/kg) verildi. Pozitif ve standart kontrol grubu tavşanlara ise 1 ml cmc solüsyonu içirildi. Sonuçlar; serum albümin konsantrasyonunun LPJE grubunda (100 mg/kg) pozitif kontrol grubundan önemli ölçüde farklı olduğunu gösterdi. En düşük kolesterol konsantrasyonu, Grup 2'nin (96.71 ± 12.03) LPJE (50mg/kg) uygulanan alt grubunda bulundu. Histopatolojik inceleme sonuçlarına göre Grup 2 istatistiksel olarak farklılık göstermedi; ancak en düşük karaciğer yağlanma derecesi LPJE (50 mg/kg) alt grubunda %26.49 ± 8.64 olarak ölçüldü. Sonuç olarak, liyofilize nar ekstresinin hepatoprotektif aktivite gösterdiği ve alkolsüz steatohepatit (NASH) tavşan modelinde en etkili dozun 50 mg/kg olduğu saptandı. Ayrıca tavşanlarda 100 mg/kg nar suyu ekstresine kadar herhangi bir yan etki tespit edilmedi.

Keywords

• Punica granatum
• antioksidan aktivite
• biyoaktivite
• alkolsüz steatohepatit

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

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