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Afyonkarahisar'da Lokal Olarak Üretilen Bazı Peynirlerdeki Brucella Spp.'nin Varlığı ve Kontaminasyon Düzeyinin belirlenmesi

Year 2014, Volume: 7 Issue: 1, 1 - 10, 23.07.2014
https://doi.org/10.5578/kvj.6928

Abstract

In this study, it was aim to determine presence and contamination level Brucella spp. in 100 home made fresh white cheese (not brined and aged cheese) with 50 home made and 50 dairy product totaly 100 tulum cheese (aged skin bag cheese) which were produced locally in Afyonkarahisar and sold at the bazaar. During this study, 200 cheese samples were evaluated in December 2011January-February, 2012. Brucella spp. from cheese samples were isolated and identified according to Farell (1974), to determine the contamination level of Brucella MPN technique was performed. Brucella spp. were isolated all sample 14.2%. from all of the cheese samples (200). Contamination level of Brucella spp. in most samples was detected as <3.6 MPN/g, in five samples 3.6 MPN/g, in three samples 6.1 MPN/g, in four samples 7.4 MPN/g and in two samples 3.0 MPN/g.Brucella spp. were detected in 6 samples (6%) of tulum cheese and 8 samples (8%) of fresh white cheese. From all of the samples totally 28 isolates were obtained and 18 (64.2%) of them were Brucella abortus, 10 (35.7%) of them were Brucella melitensis. All of the isolated strains showed resistance to amoxicilline/clavulanic acid, penicilline, tetracycline, colistin and danofloxacin

References

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  • Abbas BA, Talei AB. Isolation, identification and biotyping of Brucella spp., from milk product at basrah province. Bas J Vet Res. 2010; 9(1): 152- 162.
  • Abed Mohamad KI . Immunological, biochemical and bacteriological study on Brucella disease in human. PhD thesis, collage of science, Al- Musrtansuria Uiversity.1998.
  • Abou-Eisha AM. Brucellosis in camels and its
  • relation to public health. Assiut Vet Med J. 2000 ; 44: 54-64.
  • Advisory Committee on the Microbiological Safety of Food (ACMS). Report on microbial antibiotic resistance in relation to food safety. London: The Stationery Office.1999.
  • Akbarmehr J. The prevalence of Brucella abortus and Brucella melitensis in local cheese produced in Sarab city, Iran and its public health implication. African J Microbiol Res. 2011; 5(12): 1500-1503.
  • Al-Abbasi AM, Awan SJ, Al-Jubbory SF. Brucellosis in Baghdad, a study of 64 cases. Iraq J Microbiol. 1991; 3(1): 34-40.
  • Anonymous. Joint FAO/WHO Expert Committee, The development of new improved brucellosis vaccine, WHO/EMC/ZDI. 1997;98.14.
  • Anonymous. Manual of Standards for Diagnostic Tests and Vaccines, 12th ed., Office International des Epizooties, Paris.2003. Anonymous. Brucellosis. Available: http://www.nbc-med.org/ SiteContent/MedRef/FieldManualsmedman/Bru cellosis.htm.2010.
  • Ariza J, Pujol M, Valverde J . Brucellar sacroiliitis: findings in 63 episodes and current relevance. Clin Infect Dis. 1993; 16:761-5.
  • Awad R. Human brucellosis in the Gaza Strip, Palestine, East Mediterr. Health J. 1998; 4: 225– 233.
  • Aygen B, Doganay M, Sumerkan B, Yildiz O, Kayabas U. complications, and treatment of brucellosis: a retrospective evaluation of 480 patients. Med Malad Infect. 2002; 32: 485–493. manifestations,
  • Bacteriological Analytical Manual (BAM). Food and Drug Administration. 8th. AOAC Int, Gaithersburg, MD 20877, USA. 1998.
  • Bager F, Madsen M, Christensen J, Aarestrup FM. Avoparcin used as a growth promotor is associated with the occurrence of vancomycin- resistant Enterococcus faecium on Danish poultry and pig farms. Prev Vet Med. 1997; 31: 95–112.
  • Baykam N, Esener H, Ergonul O, Eren S, Celikbas AK, Dokuzoguz B. In vitro antimicrobial susceptibility of Brucella species. Int J Antimicrob Agents. 2004; 23:405-407.
  • Beytut E, Aydın F, Özcan K, Genç O. Kars ili ve yöresinde ineklerde mastitisin patolojik ve bakteriyolojik olarak incelenmesi. Kafkas Üniv Vet Fak Derg. 2002; 8(2): 111-112.
  • Bryan FL. Epidemiology of milkborne diseases. J
  • Food Protect. 1983; 46(7): 637–649.
  • Beuvier E, Berthaud K, Cegarra S, Dasen A, Pochet S, Buchin S, Duboz G. Ripening and quality of Swiss-type cheese made from raw, pasteurised or microfiltered milk. Int Dairy J. 1997; 7: 311-323.
  • Chomel BB, Debess EE, Mangiamele DM, Reilly KF, Farver TB, Sun RK, Barrett LR. Changing trends in the epidemiology of human brucellosis in California from 1973 to 1992: a shift toward foodborne transmission. J Infect Dis. 1994; 170:1216-23.
  • Clinical and Laboratory Standarts Institute (CLSI). Antimicrobial disc and dilution susceptibility tests for bacteria isolated from animals. Approved standart, 3rd edn. Document M31-A3 Vol. 28, No: 8. International supplement M31-s1 Vol. 24 No: 17. Wayne, PA: CLSI.2008. for
  • Claeys WL, Cardoen S, Daube G, Block JD, Dewettinck K, Dierick K., Zutter LD, Huyghebaert A, Imberechts H, Thiange P, Vandenplas Y, Herman L. Raw Or Heated Cow Milk Consumption: Review of Risks And Benefits Food Control. 2012; 31: 251-262.
  • Colonna A, Durham C, Meunier-Goddik L. Factors affecting consumers preferences for and purchasing decisions regarding pasteurised and

Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY

Year 2014, Volume: 7 Issue: 1, 1 - 10, 23.07.2014
https://doi.org/10.5578/kvj.6928

Abstract

Bu çalışmada, Aralık 2011-Ocak-Şubat 2012 döneminde, lokal olarak üretilen ve pazarda satışa sunulan 100 ev yapımı taze beyaz peynir (salamura ve olgunlaşma yapılmamış) ve 50’si ev yapımı, 50’si mandıra ürünü olmak üzere 100 tulum peyniri örneğinde Brucella spp.’nin varlığı ve kontaminasyon düzeyinin belirlenmesi amaçlanmıştır. Brucella’nın izolasyon ve identifikasyonu, Farell (1974)’e göre, kontaminasyon düzeyi En Muhtemel Sayı Tekniği’ne (EMS) göre yapıldı. Tüm örneklerden (200) %14.2 düzeyinde Brucella spp. izole edildi. Çoğu örneğin kontaminasyon düzeyi <3.6 MPN/g, 5 örneğin 3.6 MPN/g, 3 örneğin 6.1 MPN/g, 4 örneğin 7.4 MPN/g ve 2 örneğin 3.0 MPN/g olarak belirlendi. Tulum peyniri örneklerinin 6’sından, taze beyaz peynir örneklerinin 8’inden Brucella spp. saptandı. Tüm örneklerden 28 izolat elde edilirken, bunların 18’i (%64.2) Brucella abortus, 10’u (35.7) Brucella melitensis olarak identifiye edildi. Elde edilen izolatların tümü amoksisilin/klavulanik asit, penisilin, tetrasiklin, kolitsin ve danofloksasin’e dirençli olduğu belirlendi

References

  • Abbas B, Agab H. A review of camel brucellosis. Prev Vet Med. 2002;55: 47–56.
  • Abbas BA, Talei AB. Isolation, identification and biotyping of Brucella spp., from milk product at basrah province. Bas J Vet Res. 2010; 9(1): 152- 162.
  • Abed Mohamad KI . Immunological, biochemical and bacteriological study on Brucella disease in human. PhD thesis, collage of science, Al- Musrtansuria Uiversity.1998.
  • Abou-Eisha AM. Brucellosis in camels and its
  • relation to public health. Assiut Vet Med J. 2000 ; 44: 54-64.
  • Advisory Committee on the Microbiological Safety of Food (ACMS). Report on microbial antibiotic resistance in relation to food safety. London: The Stationery Office.1999.
  • Akbarmehr J. The prevalence of Brucella abortus and Brucella melitensis in local cheese produced in Sarab city, Iran and its public health implication. African J Microbiol Res. 2011; 5(12): 1500-1503.
  • Al-Abbasi AM, Awan SJ, Al-Jubbory SF. Brucellosis in Baghdad, a study of 64 cases. Iraq J Microbiol. 1991; 3(1): 34-40.
  • Anonymous. Joint FAO/WHO Expert Committee, The development of new improved brucellosis vaccine, WHO/EMC/ZDI. 1997;98.14.
  • Anonymous. Manual of Standards for Diagnostic Tests and Vaccines, 12th ed., Office International des Epizooties, Paris.2003. Anonymous. Brucellosis. Available: http://www.nbc-med.org/ SiteContent/MedRef/FieldManualsmedman/Bru cellosis.htm.2010.
  • Ariza J, Pujol M, Valverde J . Brucellar sacroiliitis: findings in 63 episodes and current relevance. Clin Infect Dis. 1993; 16:761-5.
  • Awad R. Human brucellosis in the Gaza Strip, Palestine, East Mediterr. Health J. 1998; 4: 225– 233.
  • Aygen B, Doganay M, Sumerkan B, Yildiz O, Kayabas U. complications, and treatment of brucellosis: a retrospective evaluation of 480 patients. Med Malad Infect. 2002; 32: 485–493. manifestations,
  • Bacteriological Analytical Manual (BAM). Food and Drug Administration. 8th. AOAC Int, Gaithersburg, MD 20877, USA. 1998.
  • Bager F, Madsen M, Christensen J, Aarestrup FM. Avoparcin used as a growth promotor is associated with the occurrence of vancomycin- resistant Enterococcus faecium on Danish poultry and pig farms. Prev Vet Med. 1997; 31: 95–112.
  • Baykam N, Esener H, Ergonul O, Eren S, Celikbas AK, Dokuzoguz B. In vitro antimicrobial susceptibility of Brucella species. Int J Antimicrob Agents. 2004; 23:405-407.
  • Beytut E, Aydın F, Özcan K, Genç O. Kars ili ve yöresinde ineklerde mastitisin patolojik ve bakteriyolojik olarak incelenmesi. Kafkas Üniv Vet Fak Derg. 2002; 8(2): 111-112.
  • Bryan FL. Epidemiology of milkborne diseases. J
  • Food Protect. 1983; 46(7): 637–649.
  • Beuvier E, Berthaud K, Cegarra S, Dasen A, Pochet S, Buchin S, Duboz G. Ripening and quality of Swiss-type cheese made from raw, pasteurised or microfiltered milk. Int Dairy J. 1997; 7: 311-323.
  • Chomel BB, Debess EE, Mangiamele DM, Reilly KF, Farver TB, Sun RK, Barrett LR. Changing trends in the epidemiology of human brucellosis in California from 1973 to 1992: a shift toward foodborne transmission. J Infect Dis. 1994; 170:1216-23.
  • Clinical and Laboratory Standarts Institute (CLSI). Antimicrobial disc and dilution susceptibility tests for bacteria isolated from animals. Approved standart, 3rd edn. Document M31-A3 Vol. 28, No: 8. International supplement M31-s1 Vol. 24 No: 17. Wayne, PA: CLSI.2008. for
  • Claeys WL, Cardoen S, Daube G, Block JD, Dewettinck K, Dierick K., Zutter LD, Huyghebaert A, Imberechts H, Thiange P, Vandenplas Y, Herman L. Raw Or Heated Cow Milk Consumption: Review of Risks And Benefits Food Control. 2012; 31: 251-262.
  • Colonna A, Durham C, Meunier-Goddik L. Factors affecting consumers preferences for and purchasing decisions regarding pasteurised and
There are 24 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Şebnem Pamuk This is me

Zeki Gürler This is me

Publication Date July 23, 2014
Published in Issue Year 2014 Volume: 7 Issue: 1

Cite

APA Pamuk, Ş., & Gürler, Z. (2014). Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY. Kocatepe Veterinary Journal, 7(1), 1-10. https://doi.org/10.5578/kvj.6928
AMA Pamuk Ş, Gürler Z. Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY. kvj. March 2014;7(1):1-10. doi:10.5578/kvj.6928
Chicago Pamuk, Şebnem, and Zeki Gürler. “Detection of Prevence and Contamination Level of Brucella Spp. In Local Cheese Produced in Afyonkarahisar,TURKEY”. Kocatepe Veterinary Journal 7, no. 1 (March 2014): 1-10. https://doi.org/10.5578/kvj.6928.
EndNote Pamuk Ş, Gürler Z (March 1, 2014) Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY. Kocatepe Veterinary Journal 7 1 1–10.
IEEE Ş. Pamuk and Z. Gürler, “Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY”, kvj, vol. 7, no. 1, pp. 1–10, 2014, doi: 10.5578/kvj.6928.
ISNAD Pamuk, Şebnem - Gürler, Zeki. “Detection of Prevence and Contamination Level of Brucella Spp. In Local Cheese Produced in Afyonkarahisar,TURKEY”. Kocatepe Veterinary Journal 7/1 (March 2014), 1-10. https://doi.org/10.5578/kvj.6928.
JAMA Pamuk Ş, Gürler Z. Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY. kvj. 2014;7:1–10.
MLA Pamuk, Şebnem and Zeki Gürler. “Detection of Prevence and Contamination Level of Brucella Spp. In Local Cheese Produced in Afyonkarahisar,TURKEY”. Kocatepe Veterinary Journal, vol. 7, no. 1, 2014, pp. 1-10, doi:10.5578/kvj.6928.
Vancouver Pamuk Ş, Gürler Z. Detection of Prevence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar,TURKEY. kvj. 2014;7(1):1-10.

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